In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ornamental Horticulture (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2017000400392 |
Resumo: | ABSTRACT In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3- and NH4+: T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings. |
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Ornamental Horticulture (Campinas) |
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In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratiosnitrogen sourcesmicropropagationOrchidaceaeABSTRACT In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3- and NH4+: T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings.Sociedade Brasileira de Floricultura e Plantas Ornamentais2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2017000400392Ornamental Horticulture v.23 n.4 2017reponame:Ornamental Horticulture (Campinas)instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)instacron:SBFPO10.14295/oh.v23i4.1019info:eu-repo/semantics/openAccessBertoncelli,Douglas JuniorAlves,Guilherme Augusto CitoHoshino,Rodrigo ThibesFreiria,Gustavo HenriqueFurlan,Felipe FavorettoStegani,VanessaFaria,Ricardo Tadeu deeng2018-08-31T00:00:00Zoai:scielo:S2447-536X2017000400392Revistahttp://ornamentalhorticulture.emnuvens.com.br/rbho/indexhttps://old.scielo.br/oai/scielo-oai.php||revista.ornamentalhorticulture@gmail.com2447-536X2447-536Xopendoar:2018-08-31T00:00Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO)false |
dc.title.none.fl_str_mv |
In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios |
title |
In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios |
spellingShingle |
In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios Bertoncelli,Douglas Junior nitrogen sources micropropagation Orchidaceae |
title_short |
In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios |
title_full |
In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios |
title_fullStr |
In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios |
title_full_unstemmed |
In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios |
title_sort |
In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios |
author |
Bertoncelli,Douglas Junior |
author_facet |
Bertoncelli,Douglas Junior Alves,Guilherme Augusto Cito Hoshino,Rodrigo Thibes Freiria,Gustavo Henrique Furlan,Felipe Favoretto Stegani,Vanessa Faria,Ricardo Tadeu de |
author_role |
author |
author2 |
Alves,Guilherme Augusto Cito Hoshino,Rodrigo Thibes Freiria,Gustavo Henrique Furlan,Felipe Favoretto Stegani,Vanessa Faria,Ricardo Tadeu de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Bertoncelli,Douglas Junior Alves,Guilherme Augusto Cito Hoshino,Rodrigo Thibes Freiria,Gustavo Henrique Furlan,Felipe Favoretto Stegani,Vanessa Faria,Ricardo Tadeu de |
dc.subject.por.fl_str_mv |
nitrogen sources micropropagation Orchidaceae |
topic |
nitrogen sources micropropagation Orchidaceae |
description |
ABSTRACT In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3- and NH4+: T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2017000400392 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2017000400392 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.14295/oh.v23i4.1019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Floricultura e Plantas Ornamentais |
publisher.none.fl_str_mv |
Sociedade Brasileira de Floricultura e Plantas Ornamentais |
dc.source.none.fl_str_mv |
Ornamental Horticulture v.23 n.4 2017 reponame:Ornamental Horticulture (Campinas) instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) instacron:SBFPO |
instname_str |
Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) |
instacron_str |
SBFPO |
institution |
SBFPO |
reponame_str |
Ornamental Horticulture (Campinas) |
collection |
Ornamental Horticulture (Campinas) |
repository.name.fl_str_mv |
Ornamental Horticulture (Campinas) - Sociedade Brasileira de Floricultura e Plantas Ornamentais (SBFPO) |
repository.mail.fl_str_mv |
||revista.ornamentalhorticulture@gmail.com |
_version_ |
1754734900152369152 |