In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios

Bibliographic Details
Main Author: Bertoncelli,Douglas Junior
Publication Date: 2017
Other Authors: Alves,Guilherme Augusto Cito, Hoshino,Rodrigo Thibes, Freiria,Gustavo Henrique, Furlan,Felipe Favoretto, Stegani,Vanessa, Faria,Ricardo Tadeu de
Format: Article
Language: eng
Source: Ornamental Horticulture
Download full: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2017000400392
Summary: ABSTRACT In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3- and NH4+: T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings.
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spelling In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratiosnitrogen sourcesmicropropagationOrchidaceaeABSTRACT In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3- and NH4+: T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings.Sociedade Brasileira de Floricultura e Plantas Ornamentais2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2017000400392Ornamental Horticulture v.23 n.4 2017reponame:Ornamental Horticultureinstname:Sociedade Brasileira de Floricultura e Plantas Ornamentaisinstacron:SBFPO10.14295/oh.v23i4.1019info:eu-repo/semantics/openAccessBertoncelli,Douglas JuniorAlves,Guilherme Augusto CitoHoshino,Rodrigo ThibesFreiria,Gustavo HenriqueFurlan,Felipe FavorettoStegani,VanessaFaria,Ricardo Tadeu deeng2020-04-25T22:05:12Zhttp://www.scielo.br/oai/scielo-oai.php2447-536X2447-536Xopendoar:null2020-04-26 03:22:30.28Ornamental Horticulture - Sociedade Brasileira de Floricultura e Plantas Ornamentaistrue
dc.title.none.fl_str_mv In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios
title In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios
spellingShingle In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios
Bertoncelli,Douglas Junior
nitrogen sources
micropropagation
Orchidaceae
title_short In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios
title_full In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios
title_fullStr In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios
title_full_unstemmed In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios
title_sort In vitro development of endangered Laelia marginata Lindl. in growth media containing different nitrate/ammonium ratios
author Bertoncelli,Douglas Junior
author_facet Bertoncelli,Douglas Junior
Alves,Guilherme Augusto Cito
Hoshino,Rodrigo Thibes
Freiria,Gustavo Henrique
Furlan,Felipe Favoretto
Stegani,Vanessa
Faria,Ricardo Tadeu de
author_role author
author2 Alves,Guilherme Augusto Cito
Hoshino,Rodrigo Thibes
Freiria,Gustavo Henrique
Furlan,Felipe Favoretto
Stegani,Vanessa
Faria,Ricardo Tadeu de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bertoncelli,Douglas Junior
Alves,Guilherme Augusto Cito
Hoshino,Rodrigo Thibes
Freiria,Gustavo Henrique
Furlan,Felipe Favoretto
Stegani,Vanessa
Faria,Ricardo Tadeu de
dc.subject.por.fl_str_mv nitrogen sources
micropropagation
Orchidaceae
topic nitrogen sources
micropropagation
Orchidaceae
dc.description.none.fl_txt_mv ABSTRACT In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3- and NH4+: T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings.
description ABSTRACT In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3- and NH4+: T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2017000400392
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2447-536X2017000400392
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.14295/oh.v23i4.1019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Floricultura e Plantas Ornamentais
publisher.none.fl_str_mv Sociedade Brasileira de Floricultura e Plantas Ornamentais
dc.source.none.fl_str_mv Ornamental Horticulture v.23 n.4 2017
reponame:Ornamental Horticulture
instname:Sociedade Brasileira de Floricultura e Plantas Ornamentais
instacron:SBFPO
reponame_str Ornamental Horticulture
collection Ornamental Horticulture
instname_str Sociedade Brasileira de Floricultura e Plantas Ornamentais
instacron_str SBFPO
institution SBFPO
repository.name.fl_str_mv Ornamental Horticulture - Sociedade Brasileira de Floricultura e Plantas Ornamentais
repository.mail.fl_str_mv
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