ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de fruticultura (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100101 |
Resumo: | ABSTRACT The benefits of various foods, such as fruits and vegetables, have been the focus of several studies aimed at improving welfare, health, and reducing the incidence of diseases. Therefore, the present paper objectified to investigate the presence of molecules of biotechnological and pharmacological interest in peel, seed, and pulp of Annona x atemoya Mabb. Aqueous extracts of the fruit parts were obtained with different buffers and assessed as to their protein and phenolic compounds content. The three parts of the fruit presented different proportions of these compounds when subject to different extraction conditions, with the highest concentrations of proteins being found in the seed and phenolic compounds in the peel of the studied fruit. Bioactive proteins (protease inhibitors and lectins) were detected through inhibitory tests for trypsin and chymotrypsin and hemagglutinating activity tests with human erythrocytes. A variation of 400 to 9600 inhibition units for the trypsin in the analyzed extracts, whereas for chymotrypsin a variation of 200 to 2500 in the inhibition units for the three fruit parts, considering the different extraction conditions were identified. The extracts obtained from the seeds and the peel presented titers higher than 0.9 hemagglutination units, suggesting that the by-products from the processing of A. x atemoya are potential sources of bioactive molecules. |
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ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIAAnnona x atemoyaresiduesprotease inhibitorslectinsABSTRACT The benefits of various foods, such as fruits and vegetables, have been the focus of several studies aimed at improving welfare, health, and reducing the incidence of diseases. Therefore, the present paper objectified to investigate the presence of molecules of biotechnological and pharmacological interest in peel, seed, and pulp of Annona x atemoya Mabb. Aqueous extracts of the fruit parts were obtained with different buffers and assessed as to their protein and phenolic compounds content. The three parts of the fruit presented different proportions of these compounds when subject to different extraction conditions, with the highest concentrations of proteins being found in the seed and phenolic compounds in the peel of the studied fruit. Bioactive proteins (protease inhibitors and lectins) were detected through inhibitory tests for trypsin and chymotrypsin and hemagglutinating activity tests with human erythrocytes. A variation of 400 to 9600 inhibition units for the trypsin in the analyzed extracts, whereas for chymotrypsin a variation of 200 to 2500 in the inhibition units for the three fruit parts, considering the different extraction conditions were identified. The extracts obtained from the seeds and the peel presented titers higher than 0.9 hemagglutination units, suggesting that the by-products from the processing of A. x atemoya are potential sources of bioactive molecules.Sociedade Brasileira de Fruticultura2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100101Revista Brasileira de Fruticultura v.40 n.1 2018reponame:Revista brasileira de fruticultura (Online)instname:Sociedade Brasileira de Fruticultura (SBF)instacron:SBFRU10.1590/0100-29452018098info:eu-repo/semantics/openAccessSerquiz,Alexandre CoelhoSerquiz,Raphael PaschoalPeixoto,Thayane Kerbele Oliveira das NevesSantos,Elizeu Antunes dosRocha,Hugo Alexandre Oliveiraeng2018-02-07T00:00:00Zoai:scielo:S0100-29452018000100101Revistahttp://www.scielo.br/rbfhttps://old.scielo.br/oai/scielo-oai.phprbf@fcav.unesp.br||http://rbf.org.br/1806-99670100-2945opendoar:2018-02-07T00:00Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF)false |
dc.title.none.fl_str_mv |
ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA |
title |
ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA |
spellingShingle |
ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA Serquiz,Alexandre Coelho Annona x atemoya residues protease inhibitors lectins |
title_short |
ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA |
title_full |
ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA |
title_fullStr |
ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA |
title_full_unstemmed |
ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA |
title_sort |
ASSESSMENT OF THE HEMOAGLUTINANT AND DIGESTIVE ENZYME INHIBITORY ACTIVITY OF EXTRACTS OBTAINED FROM DIFFERENT PARTS OF ATEMOIA |
author |
Serquiz,Alexandre Coelho |
author_facet |
Serquiz,Alexandre Coelho Serquiz,Raphael Paschoal Peixoto,Thayane Kerbele Oliveira das Neves Santos,Elizeu Antunes dos Rocha,Hugo Alexandre Oliveira |
author_role |
author |
author2 |
Serquiz,Raphael Paschoal Peixoto,Thayane Kerbele Oliveira das Neves Santos,Elizeu Antunes dos Rocha,Hugo Alexandre Oliveira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Serquiz,Alexandre Coelho Serquiz,Raphael Paschoal Peixoto,Thayane Kerbele Oliveira das Neves Santos,Elizeu Antunes dos Rocha,Hugo Alexandre Oliveira |
dc.subject.por.fl_str_mv |
Annona x atemoya residues protease inhibitors lectins |
topic |
Annona x atemoya residues protease inhibitors lectins |
description |
ABSTRACT The benefits of various foods, such as fruits and vegetables, have been the focus of several studies aimed at improving welfare, health, and reducing the incidence of diseases. Therefore, the present paper objectified to investigate the presence of molecules of biotechnological and pharmacological interest in peel, seed, and pulp of Annona x atemoya Mabb. Aqueous extracts of the fruit parts were obtained with different buffers and assessed as to their protein and phenolic compounds content. The three parts of the fruit presented different proportions of these compounds when subject to different extraction conditions, with the highest concentrations of proteins being found in the seed and phenolic compounds in the peel of the studied fruit. Bioactive proteins (protease inhibitors and lectins) were detected through inhibitory tests for trypsin and chymotrypsin and hemagglutinating activity tests with human erythrocytes. A variation of 400 to 9600 inhibition units for the trypsin in the analyzed extracts, whereas for chymotrypsin a variation of 200 to 2500 in the inhibition units for the three fruit parts, considering the different extraction conditions were identified. The extracts obtained from the seeds and the peel presented titers higher than 0.9 hemagglutination units, suggesting that the by-products from the processing of A. x atemoya are potential sources of bioactive molecules. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100101 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000100101 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0100-29452018098 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fruticultura |
dc.source.none.fl_str_mv |
Revista Brasileira de Fruticultura v.40 n.1 2018 reponame:Revista brasileira de fruticultura (Online) instname:Sociedade Brasileira de Fruticultura (SBF) instacron:SBFRU |
instname_str |
Sociedade Brasileira de Fruticultura (SBF) |
instacron_str |
SBFRU |
institution |
SBFRU |
reponame_str |
Revista brasileira de fruticultura (Online) |
collection |
Revista brasileira de fruticultura (Online) |
repository.name.fl_str_mv |
Revista brasileira de fruticultura (Online) - Sociedade Brasileira de Fruticultura (SBF) |
repository.mail.fl_str_mv |
rbf@fcav.unesp.br||http://rbf.org.br/ |
_version_ |
1752122494815305728 |