A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing

Detalhes bibliográficos
Autor(a) principal: Xiao,Junhua
Data de Publicação: 2006
Outros Autores: Xin,Xiujuan, Luan,Xiaohui, Dongzhi,Wei, Shengli,Yang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300028
Resumo: We describe a modified single nucleotide polymorphism (SNP) typing method based on the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). This is a simple, economical method without the need for special equipment. For most SNP loci, a common restriction endonuclease (Hind III, EcoR I or BamH I) recognizing site (RER) can be introduced into one allelic form, but not the other by two rounds of mismatched PCR. The flanking regions can be changed by as many as five bases after PCR amplification with specially designed mismatching primers so the genotypes can be distinguished after digestion of the PCR products with corresponding endonucleases.
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spelling A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typingSNP genotypingPCR-RFLPRER siteWe describe a modified single nucleotide polymorphism (SNP) typing method based on the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). This is a simple, economical method without the need for special equipment. For most SNP loci, a common restriction endonuclease (Hind III, EcoR I or BamH I) recognizing site (RER) can be introduced into one allelic form, but not the other by two rounds of mismatched PCR. The flanking regions can be changed by as many as five bases after PCR amplification with specially designed mismatching primers so the genotypes can be distinguished after digestion of the PCR products with corresponding endonucleases.Sociedade Brasileira de Genética2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300028Genetics and Molecular Biology v.29 n.3 2006reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572006000300028info:eu-repo/semantics/openAccessXiao,JunhuaXin,XiujuanLuan,XiaohuiDongzhi,WeiShengli,Yangeng2006-09-01T00:00:00Zoai:scielo:S1415-47572006000300028Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2006-09-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
title A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
spellingShingle A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
Xiao,Junhua
SNP genotyping
PCR-RFLP
RER site
title_short A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
title_full A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
title_fullStr A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
title_full_unstemmed A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
title_sort A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
author Xiao,Junhua
author_facet Xiao,Junhua
Xin,Xiujuan
Luan,Xiaohui
Dongzhi,Wei
Shengli,Yang
author_role author
author2 Xin,Xiujuan
Luan,Xiaohui
Dongzhi,Wei
Shengli,Yang
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Xiao,Junhua
Xin,Xiujuan
Luan,Xiaohui
Dongzhi,Wei
Shengli,Yang
dc.subject.por.fl_str_mv SNP genotyping
PCR-RFLP
RER site
topic SNP genotyping
PCR-RFLP
RER site
description We describe a modified single nucleotide polymorphism (SNP) typing method based on the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). This is a simple, economical method without the need for special equipment. For most SNP loci, a common restriction endonuclease (Hind III, EcoR I or BamH I) recognizing site (RER) can be introduced into one allelic form, but not the other by two rounds of mismatched PCR. The flanking regions can be changed by as many as five bases after PCR amplification with specially designed mismatching primers so the genotypes can be distinguished after digestion of the PCR products with corresponding endonucleases.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300028
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300028
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572006000300028
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.29 n.3 2006
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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