The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018 |
Resumo: | The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora. |
id |
SBG-1_1f9666ce7ac8159335971deffd8d68eb |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572010000100018 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)genetic diversityintraspecific variabilityITSPassifloraphylogeographyThe discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora.Sociedade Brasileira de Genética2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018Genetics and Molecular Biology v.33 n.1 2010reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000101info:eu-repo/semantics/openAccessMäder,GeraldoZamberlan,Priscilla M.Fagundes,Nelson J.R.Magnus,TielliSalzano,Francisco M.Bonatto,Sandro L.Freitas,Loreta B.eng2010-02-12T00:00:00Zoai:scielo:S1415-47572010000100018Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2010-02-12T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
title |
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
spellingShingle |
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) Mäder,Geraldo genetic diversity intraspecific variability ITS Passiflora phylogeography |
title_short |
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
title_full |
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
title_fullStr |
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
title_full_unstemmed |
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
title_sort |
The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) |
author |
Mäder,Geraldo |
author_facet |
Mäder,Geraldo Zamberlan,Priscilla M. Fagundes,Nelson J.R. Magnus,Tielli Salzano,Francisco M. Bonatto,Sandro L. Freitas,Loreta B. |
author_role |
author |
author2 |
Zamberlan,Priscilla M. Fagundes,Nelson J.R. Magnus,Tielli Salzano,Francisco M. Bonatto,Sandro L. Freitas,Loreta B. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Mäder,Geraldo Zamberlan,Priscilla M. Fagundes,Nelson J.R. Magnus,Tielli Salzano,Francisco M. Bonatto,Sandro L. Freitas,Loreta B. |
dc.subject.por.fl_str_mv |
genetic diversity intraspecific variability ITS Passiflora phylogeography |
topic |
genetic diversity intraspecific variability ITS Passiflora phylogeography |
description |
The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572009005000101 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.33 n.1 2010 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122382701559808 |