The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)

Detalhes bibliográficos
Autor(a) principal: Mäder,Geraldo
Data de Publicação: 2010
Outros Autores: Zamberlan,Priscilla M., Fagundes,Nelson J.R., Magnus,Tielli, Salzano,Francisco M., Bonatto,Sandro L., Freitas,Loreta B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018
Resumo: The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora.
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spelling The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)genetic diversityintraspecific variabilityITSPassifloraphylogeographyThe discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora.Sociedade Brasileira de Genética2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018Genetics and Molecular Biology v.33 n.1 2010reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000101info:eu-repo/semantics/openAccessMäder,GeraldoZamberlan,Priscilla M.Fagundes,Nelson J.R.Magnus,TielliSalzano,Francisco M.Bonatto,Sandro L.Freitas,Loreta B.eng2010-02-12T00:00:00Zoai:scielo:S1415-47572010000100018Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2010-02-12T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
spellingShingle The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
Mäder,Geraldo
genetic diversity
intraspecific variability
ITS
Passiflora
phylogeography
title_short The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title_full The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title_fullStr The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title_full_unstemmed The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
title_sort The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
author Mäder,Geraldo
author_facet Mäder,Geraldo
Zamberlan,Priscilla M.
Fagundes,Nelson J.R.
Magnus,Tielli
Salzano,Francisco M.
Bonatto,Sandro L.
Freitas,Loreta B.
author_role author
author2 Zamberlan,Priscilla M.
Fagundes,Nelson J.R.
Magnus,Tielli
Salzano,Francisco M.
Bonatto,Sandro L.
Freitas,Loreta B.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mäder,Geraldo
Zamberlan,Priscilla M.
Fagundes,Nelson J.R.
Magnus,Tielli
Salzano,Francisco M.
Bonatto,Sandro L.
Freitas,Loreta B.
dc.subject.por.fl_str_mv genetic diversity
intraspecific variability
ITS
Passiflora
phylogeography
topic genetic diversity
intraspecific variability
ITS
Passiflora
phylogeography
description The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic variation in Passiflora.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572009005000101
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.33 n.1 2010
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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