Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates

Detalhes bibliográficos
Autor(a) principal: Timenetsky, Jorge
Data de Publicação: 2011
Outros Autores: Marques, Lucas Miranda, Buzinhani, Melissa, Guimarães, Ana Marcia de Sá, Oliveira, Rosângela Claret de, Yamaguti, Maurício, Marques, R. C. P., Farias, S. T., Lopes Neto, R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5450
Resumo: texto xompleto: acesso restrito. p. 205–211.
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spelling Timenetsky, JorgeMarques, Lucas MirandaBuzinhani, MelissaGuimarães, Ana Marcia de SáOliveira, Rosângela Claret deYamaguti, MaurícioMarques, R. C. P.Farias, S. T.Lopes Neto, R.Timenetsky, JorgeMarques, Lucas MirandaBuzinhani, MelissaGuimarães, Ana Marcia de SáOliveira, Rosângela Claret deYamaguti, MaurícioMarques, R. C. P.Farias, S. T.Lopes Neto, R.2012-02-25T15:56:20Z20111873-2542http://www.repositorio.ufba.br/ri/handle/ri/5450v. 152.texto xompleto: acesso restrito. p. 205–211.Ureaplasma diversum infection in bulls may result in seminal vesiculitis, balanoposthitis and alterations in spermatozoids. In cows, it can cause placentitis, fetal alveolitis, abortion and the birth of weak calves. U. diversum ATCC 49782 (serogroups A), ATCC 49783 (serogroup C) and 34 field isolates were used for this study. These microorganisms were submitted to Polymerase Chain Reaction for 16S gene sequence determination using Taq High Fidelity and the products were purified and bi-directionally sequenced. Using the sequence obtained, a fragment containing four hypervariable regions was selected and nucleotide polymorphisms were identified based on their position within the 16S rRNA gene. Forty-four single nucleotide polymorphisms (SNP) were detected. The genotypic variability of the 16S rRNA gene of U. diversum isolates shows that the taxonomy classification of these organisms is likely much more complex than previously described and that 16S rRNA gene sequencing may be used to suggest an epidemiologic pattern of different origin strains.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-25T15:56:20Z No. of bitstreams: 1 npsA.tmp.pdf: 172593 bytes, checksum: f3480c3b9ac425ef128447d72e949d7a (MD5)Made available in DSpace on 2012-02-25T15:56:20Z (GMT). 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dc.title.pt_BR.fl_str_mv Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
dc.title.alternative.pt_BR.fl_str_mv Veterinary Microbiology
title Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
spellingShingle Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
Timenetsky, Jorge
Ureaplasma diversum
Intraspecific variation
16S rRNA gene
title_short Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
title_full Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
title_fullStr Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
title_full_unstemmed Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
title_sort Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates
author Timenetsky, Jorge
author_facet Timenetsky, Jorge
Marques, Lucas Miranda
Buzinhani, Melissa
Guimarães, Ana Marcia de Sá
Oliveira, Rosângela Claret de
Yamaguti, Maurício
Marques, R. C. P.
Farias, S. T.
Lopes Neto, R.
author_role author
author2 Marques, Lucas Miranda
Buzinhani, Melissa
Guimarães, Ana Marcia de Sá
Oliveira, Rosângela Claret de
Yamaguti, Maurício
Marques, R. C. P.
Farias, S. T.
Lopes Neto, R.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Timenetsky, Jorge
Marques, Lucas Miranda
Buzinhani, Melissa
Guimarães, Ana Marcia de Sá
Oliveira, Rosângela Claret de
Yamaguti, Maurício
Marques, R. C. P.
Farias, S. T.
Lopes Neto, R.
Timenetsky, Jorge
Marques, Lucas Miranda
Buzinhani, Melissa
Guimarães, Ana Marcia de Sá
Oliveira, Rosângela Claret de
Yamaguti, Maurício
Marques, R. C. P.
Farias, S. T.
Lopes Neto, R.
dc.subject.por.fl_str_mv Ureaplasma diversum
Intraspecific variation
16S rRNA gene
topic Ureaplasma diversum
Intraspecific variation
16S rRNA gene
description texto xompleto: acesso restrito. p. 205–211.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-02-25T15:56:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5450
dc.identifier.issn.none.fl_str_mv 1873-2542
dc.identifier.number.pt_BR.fl_str_mv v. 152.
identifier_str_mv 1873-2542
v. 152.
url http://www.repositorio.ufba.br/ri/handle/ri/5450
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv doi:10.1016/j.vetmic.2011.04.007
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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