Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)

Detalhes bibliográficos
Autor(a) principal: Guarido,Paula Carolina Paes
Data de Publicação: 2012
Outros Autores: Paula,Adriano Alves de, Silva,Carlos Roberto Maximiano da, Rodriguez,Carmen, Vanzela,André Luís Laforga
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300012
Resumo: Heterochromatin comprises a fraction of the genome usually with highly repeated DNA sequences and lacks of functional genes. This region can be revealed by using Giemsa C-banding, fluorochrome staining and cytomolecular tools. Some plant species are of particular interest through having a special type of heterochromatin denominated the cold-sensitive region (CSR). Independent of other chromosomal regions, when biological materials are subjected to low temperatures (about 0 °C), CSRs appear slightly stained and decondensed. In this study, we used Cestrum strigilatum (Solanaceae) to understand some aspects of CSR condensation associated with cytosine methylation levels, and to compare the behavior of different heterochromatin types of this species, when subjected to low temperatures.
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spelling Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)anti-5-methylcytosineCestrumchromosomesCMA/DAPI bandingheterochromatinimmunolabelingHeterochromatin comprises a fraction of the genome usually with highly repeated DNA sequences and lacks of functional genes. This region can be revealed by using Giemsa C-banding, fluorochrome staining and cytomolecular tools. Some plant species are of particular interest through having a special type of heterochromatin denominated the cold-sensitive region (CSR). Independent of other chromosomal regions, when biological materials are subjected to low temperatures (about 0 °C), CSRs appear slightly stained and decondensed. In this study, we used Cestrum strigilatum (Solanaceae) to understand some aspects of CSR condensation associated with cytosine methylation levels, and to compare the behavior of different heterochromatin types of this species, when subjected to low temperatures.Sociedade Brasileira de Genética2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300012Genetics and Molecular Biology v.35 n.2 2012reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572012005000026info:eu-repo/semantics/openAccessGuarido,Paula Carolina PaesPaula,Adriano Alves deSilva,Carlos Roberto Maximiano daRodriguez,CarmenVanzela,André Luís Laforgaeng2012-06-19T00:00:00Zoai:scielo:S1415-47572012000300012Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2012-06-19T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)
title Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)
spellingShingle Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)
Guarido,Paula Carolina Paes
anti-5-methylcytosine
Cestrum
chromosomes
CMA/DAPI banding
heterochromatin
immunolabeling
title_short Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)
title_full Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)
title_fullStr Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)
title_full_unstemmed Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)
title_sort Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae)
author Guarido,Paula Carolina Paes
author_facet Guarido,Paula Carolina Paes
Paula,Adriano Alves de
Silva,Carlos Roberto Maximiano da
Rodriguez,Carmen
Vanzela,André Luís Laforga
author_role author
author2 Paula,Adriano Alves de
Silva,Carlos Roberto Maximiano da
Rodriguez,Carmen
Vanzela,André Luís Laforga
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Guarido,Paula Carolina Paes
Paula,Adriano Alves de
Silva,Carlos Roberto Maximiano da
Rodriguez,Carmen
Vanzela,André Luís Laforga
dc.subject.por.fl_str_mv anti-5-methylcytosine
Cestrum
chromosomes
CMA/DAPI banding
heterochromatin
immunolabeling
topic anti-5-methylcytosine
Cestrum
chromosomes
CMA/DAPI banding
heterochromatin
immunolabeling
description Heterochromatin comprises a fraction of the genome usually with highly repeated DNA sequences and lacks of functional genes. This region can be revealed by using Giemsa C-banding, fluorochrome staining and cytomolecular tools. Some plant species are of particular interest through having a special type of heterochromatin denominated the cold-sensitive region (CSR). Independent of other chromosomal regions, when biological materials are subjected to low temperatures (about 0 °C), CSRs appear slightly stained and decondensed. In this study, we used Cestrum strigilatum (Solanaceae) to understand some aspects of CSR condensation associated with cytosine methylation levels, and to compare the behavior of different heterochromatin types of this species, when subjected to low temperatures.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572012005000026
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.35 n.2 2012
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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