Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA

Detalhes bibliográficos
Autor(a) principal: Fregonezi,Jéferson Nunes
Data de Publicação: 2006
Outros Autores: Fernandes,Thiago, Torezan,José Marcelo Domingues, Vieira,Ana Odete S., Vanzela,André Luís Laforga
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019
Resumo: We studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n = 2x = 16, with, except for the eighth acrocentric pair, a predominance of meta- and submetacentric chromosomes and various heterochromatin classes. Heterochromatic types previously unreported in Cestrum as neutral C-CMA3(0)/DAPI0 bands, CMA3+ bands not associated with NORs, and C-Giemsa/CSR/DAPI- bands were found. The heterochromatic blocks varied in size, number, position and composition. The 45S rDNA probe preferentially located in the terminal and subterminal regions of some chromosomes, while 5S rDNA appeared close to the centromere of the long arm of pair 8. These results suggest that karyotype differentiation can occur mainly due to changes in repetitive DNA, with little modification in the general composition of the conventionally stained karyotype.
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spelling Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNACestrumchromosome bandingCSRskaryotype evolutionrDNA probesWe studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n = 2x = 16, with, except for the eighth acrocentric pair, a predominance of meta- and submetacentric chromosomes and various heterochromatin classes. Heterochromatic types previously unreported in Cestrum as neutral C-CMA3(0)/DAPI0 bands, CMA3+ bands not associated with NORs, and C-Giemsa/CSR/DAPI- bands were found. The heterochromatic blocks varied in size, number, position and composition. The 45S rDNA probe preferentially located in the terminal and subterminal regions of some chromosomes, while 5S rDNA appeared close to the centromere of the long arm of pair 8. These results suggest that karyotype differentiation can occur mainly due to changes in repetitive DNA, with little modification in the general composition of the conventionally stained karyotype.Sociedade Brasileira de Genética2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019Genetics and Molecular Biology v.29 n.1 2006reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572006000100019info:eu-repo/semantics/openAccessFregonezi,Jéferson NunesFernandes,ThiagoTorezan,José Marcelo DominguesVieira,Ana Odete S.Vanzela,André Luís Laforgaeng2006-03-10T00:00:00Zoai:scielo:S1415-47572006000100019Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2006-03-10T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
title Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
spellingShingle Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
Fregonezi,Jéferson Nunes
Cestrum
chromosome banding
CSRs
karyotype evolution
rDNA probes
title_short Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
title_full Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
title_fullStr Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
title_full_unstemmed Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
title_sort Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
author Fregonezi,Jéferson Nunes
author_facet Fregonezi,Jéferson Nunes
Fernandes,Thiago
Torezan,José Marcelo Domingues
Vieira,Ana Odete S.
Vanzela,André Luís Laforga
author_role author
author2 Fernandes,Thiago
Torezan,José Marcelo Domingues
Vieira,Ana Odete S.
Vanzela,André Luís Laforga
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fregonezi,Jéferson Nunes
Fernandes,Thiago
Torezan,José Marcelo Domingues
Vieira,Ana Odete S.
Vanzela,André Luís Laforga
dc.subject.por.fl_str_mv Cestrum
chromosome banding
CSRs
karyotype evolution
rDNA probes
topic Cestrum
chromosome banding
CSRs
karyotype evolution
rDNA probes
description We studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n = 2x = 16, with, except for the eighth acrocentric pair, a predominance of meta- and submetacentric chromosomes and various heterochromatin classes. Heterochromatic types previously unreported in Cestrum as neutral C-CMA3(0)/DAPI0 bands, CMA3+ bands not associated with NORs, and C-Giemsa/CSR/DAPI- bands were found. The heterochromatic blocks varied in size, number, position and composition. The 45S rDNA probe preferentially located in the terminal and subterminal regions of some chromosomes, while 5S rDNA appeared close to the centromere of the long arm of pair 8. These results suggest that karyotype differentiation can occur mainly due to changes in repetitive DNA, with little modification in the general composition of the conventionally stained karyotype.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572006000100019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.29 n.1 2006
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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