Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100003 |
Resumo: | Fourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.034; Kruskal-Wallis test). This result suggests that the Rsa I polymorphism may be involved in Pit-1 protein expression or function, which in turn may influence GH transcription and expression. Thus, the Rsa I PIT1 gene polymorphism in this pig line may be used as a molecular marker to identify higher GH expression and possibly select for carcass and performance traits affected by GH. |
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Genetics and Molecular Biology |
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Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glandsPIT1 genegrowth hormone mRNAsemi-quantitative RT-PCRpigFourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.034; Kruskal-Wallis test). This result suggests that the Rsa I polymorphism may be involved in Pit-1 protein expression or function, which in turn may influence GH transcription and expression. Thus, the Rsa I PIT1 gene polymorphism in this pig line may be used as a molecular marker to identify higher GH expression and possibly select for carcass and performance traits affected by GH.Sociedade Brasileira de Genética2005-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100003Genetics and Molecular Biology v.28 n.1 2005reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572005000100003info:eu-repo/semantics/openAccessFranco,Maurício MachaimAntunes,Robson CarlosOliveira,Katiana Mello dePereira,Cícero DonizeteBiase,Fernando HenriqueNunes,Francis de Morais FrancoGoulart,Luiz Ricardoeng2005-09-08T00:00:00Zoai:scielo:S1415-47572005000100003Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2005-09-08T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands |
title |
Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands |
spellingShingle |
Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands Franco,Maurício Machaim PIT1 gene growth hormone mRNA semi-quantitative RT-PCR pig |
title_short |
Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands |
title_full |
Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands |
title_fullStr |
Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands |
title_full_unstemmed |
Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands |
title_sort |
Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands |
author |
Franco,Maurício Machaim |
author_facet |
Franco,Maurício Machaim Antunes,Robson Carlos Oliveira,Katiana Mello de Pereira,Cícero Donizete Biase,Fernando Henrique Nunes,Francis de Morais Franco Goulart,Luiz Ricardo |
author_role |
author |
author2 |
Antunes,Robson Carlos Oliveira,Katiana Mello de Pereira,Cícero Donizete Biase,Fernando Henrique Nunes,Francis de Morais Franco Goulart,Luiz Ricardo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Franco,Maurício Machaim Antunes,Robson Carlos Oliveira,Katiana Mello de Pereira,Cícero Donizete Biase,Fernando Henrique Nunes,Francis de Morais Franco Goulart,Luiz Ricardo |
dc.subject.por.fl_str_mv |
PIT1 gene growth hormone mRNA semi-quantitative RT-PCR pig |
topic |
PIT1 gene growth hormone mRNA semi-quantitative RT-PCR pig |
description |
Fourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.034; Kruskal-Wallis test). This result suggests that the Rsa I polymorphism may be involved in Pit-1 protein expression or function, which in turn may influence GH transcription and expression. Thus, the Rsa I PIT1 gene polymorphism in this pig line may be used as a molecular marker to identify higher GH expression and possibly select for carcass and performance traits affected by GH. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572005000100003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.28 n.1 2005 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122379423711232 |