AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development

Detalhes bibliográficos
Autor(a) principal: Gerhardt,Liliane B. de A.
Data de Publicação: 2004
Outros Autores: Magioli,Cláudia, Perez,Ana B.U.C.M., Margis,Rogério, Sachetto-Martins,Gilberto, Margis-Pinheiro,Márcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020
Resumo: The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of transcription. A negative regulatory region located between portions -1083 and -600 was also observed.
id SBG-1_2cdbcafbf7b83ab470a53201a798232a
oai_identifier_str oai:scielo:S1415-47572004000100020
network_acronym_str SBG-1
network_name_str Genetics and Molecular Biology
repository_id_str
spelling AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo developmentabiotic stressChia4 Arabidopsis thaliana endochitinaseembryogenesisgene expressionpromoterThe expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of transcription. A negative regulatory region located between portions -1083 and -600 was also observed.Sociedade Brasileira de Genética2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020Genetics and Molecular Biology v.27 n.1 2004reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572004000100020info:eu-repo/semantics/openAccessGerhardt,Liliane B. de A.Magioli,CláudiaPerez,Ana B.U.C.M.Margis,RogérioSachetto-Martins,GilbertoMargis-Pinheiro,Márciaeng2004-04-13T00:00:00Zoai:scielo:S1415-47572004000100020Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2004-04-13T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
title AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
spellingShingle AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
Gerhardt,Liliane B. de A.
abiotic stress
Chia4 Arabidopsis thaliana endochitinase
embryogenesis
gene expression
promoter
title_short AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
title_full AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
title_fullStr AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
title_full_unstemmed AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
title_sort AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development
author Gerhardt,Liliane B. de A.
author_facet Gerhardt,Liliane B. de A.
Magioli,Cláudia
Perez,Ana B.U.C.M.
Margis,Rogério
Sachetto-Martins,Gilberto
Margis-Pinheiro,Márcia
author_role author
author2 Magioli,Cláudia
Perez,Ana B.U.C.M.
Margis,Rogério
Sachetto-Martins,Gilberto
Margis-Pinheiro,Márcia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gerhardt,Liliane B. de A.
Magioli,Cláudia
Perez,Ana B.U.C.M.
Margis,Rogério
Sachetto-Martins,Gilberto
Margis-Pinheiro,Márcia
dc.subject.por.fl_str_mv abiotic stress
Chia4 Arabidopsis thaliana endochitinase
embryogenesis
gene expression
promoter
topic abiotic stress
Chia4 Arabidopsis thaliana endochitinase
embryogenesis
gene expression
promoter
description The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of transcription. A negative regulatory region located between portions -1083 and -600 was also observed.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572004000100020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.27 n.1 2004
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
_version_ 1752122378994843648

Registros relacionados