DNA damage, oxidative stress, and inflammation in children with celiac disease

• Autor(a) principal: Maluf,Sharbel Weidner
• Data de Publicação: 2020
• Outros Autores: Wilhelm Filho,Danilo, Parisotto,Eduardo Benedetti, Medeiros,Guilherme da Silva de, Pereira,Carolina Hilgert Jacobsen, Maraslis,Flora Troina, Dornelles Schoeller,Carlos C., Rosa,Julia Savan da, Fröde,Tânia Silvia
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Genetics and Molecular Biology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400112
• topic: Celiac disease; biochemical markers; genotoxicity; reactive oxygen species (ROS); inflammatory markers

Abstract The objective of this study was to evaluate the level of genomic instability in patients with celiac disease and to establish a relationship between inflammation, oxidative stress, and DNA damage in these patients. Myeloperoxidase (MPO) activity, adenosine deaminase, nitric oxide (NOx), thiobarbituric acid, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and DNA damage were evaluated in peripheral blood samples from 47 celiac disease patients and 31 controls. Patients with celiac disease presented higher levels of DNA damage in comparison to controls (p=0.023). This difference was also observed for markers of oxidative stress, such as CAT (p=0.011) and SOD (p=0.013), and inflammatory markers such as MPO (p < 0.001) and NOx (p=0.009). Positive correlations were found between DNA damage levels and the values of CAT (r=0.405; p=0.009) and SOD (r=0.516; p < 0.001). Positive correlations were also found between GPx and NOx (r=0.349; p=0.030) and MPO and NOx (r=0.239; p=0.039). CAT and NOx showed a negative correlation (r= −0.315; p=0.042). In conclusion, intestinal inflammation can have systemic effects, causing an imbalance between oxidant and antioxidant markers, which may promote increased levels of DNA damage.