Expression pattern of drought stress marker genes in soybean roots under two water deficit systems

Detalhes bibliográficos
Autor(a) principal: Neves-Borges,Anna Cristina
Data de Publicação: 2012
Outros Autores: Guimarães-Dias,Fábia, Cruz,Fernanda, Mesquita,Rosilene Oliveira, Nepomuceno,Alexandre Lima, Romano,Eduardo, Loureiro,Marcelo Ehlers, Grossi-de-Sá,Maria de Fátima, Alves-Ferreira,Márcio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200003
Resumo: The study of tolerance mechanisms for drought stress in soybean is fundamental to the understanding and development of tolerant varieties. Using in silico analysis, four marker genes involved in the classical ABA-dependent and ABA-independent pathways of drought response were identified in the Glycine max genome in the present work. The expression profiles of the marker genes ERD1-like, GmaxRD20A-like, GmaxRD22-like and GmaxRD29B-like were investigated by qPCR in root samples of drought sensitive and tolerant soybean cultivars (BR 16 and Embrapa 48, respectively), submitted to water deficit conditions in hydroponic and pot-based systems. Among the four putative soybean homologs to Arabidopsis genes investigated herein, only GmaxRD29B-like was not regulated by water deficit stress. Distinct expression profiles and different induction levels were observed among the genes, as well as between the two drought-inducing systems. Our results showed contrasting gene expression responses for the GmaxRD20A-like and GmaxRD22-like genes. GmaxRD20A-like was highly induced by continuous drought acclimating conditions, whereas GmaxRD22-like responses decreased after abrupt water deprivation. GmaxERD1-like showed a different expression profile for the cultivars in each system. Conversely, GmaxRD20A-like and GmaxRD22-like genes exhibited similar expression levels in tolerant plants in both systems.
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spelling Expression pattern of drought stress marker genes in soybean roots under two water deficit systemswater deficitdrought adaptationmarker genesGlycine maxThe study of tolerance mechanisms for drought stress in soybean is fundamental to the understanding and development of tolerant varieties. Using in silico analysis, four marker genes involved in the classical ABA-dependent and ABA-independent pathways of drought response were identified in the Glycine max genome in the present work. The expression profiles of the marker genes ERD1-like, GmaxRD20A-like, GmaxRD22-like and GmaxRD29B-like were investigated by qPCR in root samples of drought sensitive and tolerant soybean cultivars (BR 16 and Embrapa 48, respectively), submitted to water deficit conditions in hydroponic and pot-based systems. Among the four putative soybean homologs to Arabidopsis genes investigated herein, only GmaxRD29B-like was not regulated by water deficit stress. Distinct expression profiles and different induction levels were observed among the genes, as well as between the two drought-inducing systems. Our results showed contrasting gene expression responses for the GmaxRD20A-like and GmaxRD22-like genes. GmaxRD20A-like was highly induced by continuous drought acclimating conditions, whereas GmaxRD22-like responses decreased after abrupt water deprivation. GmaxERD1-like showed a different expression profile for the cultivars in each system. Conversely, GmaxRD20A-like and GmaxRD22-like genes exhibited similar expression levels in tolerant plants in both systems.Sociedade Brasileira de Genética2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200003Genetics and Molecular Biology v.35 n.1 suppl.1 2012reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572012000200003info:eu-repo/semantics/openAccessNeves-Borges,Anna CristinaGuimarães-Dias,FábiaCruz,FernandaMesquita,Rosilene OliveiraNepomuceno,Alexandre LimaRomano,EduardoLoureiro,Marcelo EhlersGrossi-de-Sá,Maria de FátimaAlves-Ferreira,Márcioeng2012-06-01T00:00:00Zoai:scielo:S1415-47572012000200003Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2012-06-01T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
title Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
spellingShingle Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
Neves-Borges,Anna Cristina
water deficit
drought adaptation
marker genes
Glycine max
title_short Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
title_full Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
title_fullStr Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
title_full_unstemmed Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
title_sort Expression pattern of drought stress marker genes in soybean roots under two water deficit systems
author Neves-Borges,Anna Cristina
author_facet Neves-Borges,Anna Cristina
Guimarães-Dias,Fábia
Cruz,Fernanda
Mesquita,Rosilene Oliveira
Nepomuceno,Alexandre Lima
Romano,Eduardo
Loureiro,Marcelo Ehlers
Grossi-de-Sá,Maria de Fátima
Alves-Ferreira,Márcio
author_role author
author2 Guimarães-Dias,Fábia
Cruz,Fernanda
Mesquita,Rosilene Oliveira
Nepomuceno,Alexandre Lima
Romano,Eduardo
Loureiro,Marcelo Ehlers
Grossi-de-Sá,Maria de Fátima
Alves-Ferreira,Márcio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Neves-Borges,Anna Cristina
Guimarães-Dias,Fábia
Cruz,Fernanda
Mesquita,Rosilene Oliveira
Nepomuceno,Alexandre Lima
Romano,Eduardo
Loureiro,Marcelo Ehlers
Grossi-de-Sá,Maria de Fátima
Alves-Ferreira,Márcio
dc.subject.por.fl_str_mv water deficit
drought adaptation
marker genes
Glycine max
topic water deficit
drought adaptation
marker genes
Glycine max
description The study of tolerance mechanisms for drought stress in soybean is fundamental to the understanding and development of tolerant varieties. Using in silico analysis, four marker genes involved in the classical ABA-dependent and ABA-independent pathways of drought response were identified in the Glycine max genome in the present work. The expression profiles of the marker genes ERD1-like, GmaxRD20A-like, GmaxRD22-like and GmaxRD29B-like were investigated by qPCR in root samples of drought sensitive and tolerant soybean cultivars (BR 16 and Embrapa 48, respectively), submitted to water deficit conditions in hydroponic and pot-based systems. Among the four putative soybean homologs to Arabidopsis genes investigated herein, only GmaxRD29B-like was not regulated by water deficit stress. Distinct expression profiles and different induction levels were observed among the genes, as well as between the two drought-inducing systems. Our results showed contrasting gene expression responses for the GmaxRD20A-like and GmaxRD22-like genes. GmaxRD20A-like was highly induced by continuous drought acclimating conditions, whereas GmaxRD22-like responses decreased after abrupt water deprivation. GmaxERD1-like showed a different expression profile for the cultivars in each system. Conversely, GmaxRD20A-like and GmaxRD22-like genes exhibited similar expression levels in tolerant plants in both systems.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572012000200003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.35 n.1 suppl.1 2012
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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