Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots

Detalhes bibliográficos
Autor(a) principal: Loureiro, Marcelo E.
Data de Publicação: 2016
Outros Autores: Dias, Fábia Guimarães, Borges, Anna C. Neves, Conforte, Alessandra J., Kampmann, Leonardo Giovanella, Ferreira, André V. J., Amorim, Regina M. S., Benevent, Magda A., Sá, Maria Eugênia Lisei de, Mesquita, Rosilene O., Rodrigues, Fabiana A., Nepomuceno, Alexandre L., Romano, Eduardo, Sá, Maria Fátima Grossi de, Ferreira, Márcio Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1007/s11105-016-0993-z
http://www.locus.ufv.br/handle/123456789/21998
Resumo: Soybean (Glycine max) is one of the major world commodities. In order to increase the soybean yields, it has been searched drought-tolerant cultivars, once the drought is the major constraint to soybean grown. Therefore, it is crucial to elucidate the molecular mechanisms associated with drought tolerance. Here, the in silico approach allowed us to identify 12 genes belonging to six different transcription factor families in soybean that have been associated with key events on drought response. The expression pattern of each gene was investigated by qPCR in root samples of drought-sensitive and drought-tolerant cultivars undergoing drought stress in pot-based (PSys) and hydroponic (HSys) cultivation systems. GmaxMYC2-like 2 was induced under abrupt drought conditions in HSys in both cultivars, whereas GmaxAREB1-like 1 and GmaxDREB2A-like were highly induced only in the PSys. However, GmaxMYB2-like 1, GmaxMYB2-like 2, GmaxRD26/NAC-like 1, GmaxRD26/NAC-like 2, GmaxAREB1-like 2, and GmaxDREB1A-like were upregulated in both systems, while the GmaxHB6-like and GmaxHB13-like are repressed under all of the investigated conditions. Exogenous abscisic acid (ABA) treatment was used to identify those genes belonging to the ABA-dependent drought response. The genes identified in this work have potential application for the improvement of drought resistance in soybean and markers for breeding programs.
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spelling Loureiro, Marcelo E.Dias, Fábia GuimarãesBorges, Anna C. NevesConforte, Alessandra J.Kampmann, Leonardo GiovanellaFerreira, André V. J.Amorim, Regina M. S.Benevent, Magda A.Sá, Maria Eugênia Lisei deMesquita, Rosilene O.Rodrigues, Fabiana A.Nepomuceno, Alexandre L.Romano, EduardoSá, Maria Fátima Grossi deFerreira, Márcio Alves2018-09-25T18:17:02Z2018-09-25T18:17:02Z2016-121572-9818https://doi.org/10.1007/s11105-016-0993-zhttp://www.locus.ufv.br/handle/123456789/21998Soybean (Glycine max) is one of the major world commodities. In order to increase the soybean yields, it has been searched drought-tolerant cultivars, once the drought is the major constraint to soybean grown. Therefore, it is crucial to elucidate the molecular mechanisms associated with drought tolerance. Here, the in silico approach allowed us to identify 12 genes belonging to six different transcription factor families in soybean that have been associated with key events on drought response. The expression pattern of each gene was investigated by qPCR in root samples of drought-sensitive and drought-tolerant cultivars undergoing drought stress in pot-based (PSys) and hydroponic (HSys) cultivation systems. GmaxMYC2-like 2 was induced under abrupt drought conditions in HSys in both cultivars, whereas GmaxAREB1-like 1 and GmaxDREB2A-like were highly induced only in the PSys. However, GmaxMYB2-like 1, GmaxMYB2-like 2, GmaxRD26/NAC-like 1, GmaxRD26/NAC-like 2, GmaxAREB1-like 2, and GmaxDREB1A-like were upregulated in both systems, while the GmaxHB6-like and GmaxHB13-like are repressed under all of the investigated conditions. Exogenous abscisic acid (ABA) treatment was used to identify those genes belonging to the ABA-dependent drought response. The genes identified in this work have potential application for the improvement of drought resistance in soybean and markers for breeding programs.engPlant Molecular Biology ReporterVolume 34, Issue 6, p. 1167–1180, December 2016Springer USinfo:eu-repo/semantics/openAccessDrought adaptationGlycine maxMarker assistance selectionTranscription factorsWater deficitDifferential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean rootsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf1585766https://locus.ufv.br//bitstream/123456789/21998/1/artigo.pdfb5c8e22e6fbd68595aab558d06b3b26aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/21998/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg4693https://locus.ufv.br//bitstream/123456789/21998/3/artigo.pdf.jpg4fba357481c73fd176f04ed8f5ef74fdMD53123456789/219982018-09-25 23:00:43.206oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-09-26T02:00:43LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots
title Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots
spellingShingle Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots
Loureiro, Marcelo E.
Drought adaptation
Glycine max
Marker assistance selection
Transcription factors
Water deficit
title_short Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots
title_full Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots
title_fullStr Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots
title_full_unstemmed Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots
title_sort Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots
author Loureiro, Marcelo E.
author_facet Loureiro, Marcelo E.
Dias, Fábia Guimarães
Borges, Anna C. Neves
Conforte, Alessandra J.
Kampmann, Leonardo Giovanella
Ferreira, André V. J.
Amorim, Regina M. S.
Benevent, Magda A.
Sá, Maria Eugênia Lisei de
Mesquita, Rosilene O.
Rodrigues, Fabiana A.
Nepomuceno, Alexandre L.
Romano, Eduardo
Sá, Maria Fátima Grossi de
Ferreira, Márcio Alves
author_role author
author2 Dias, Fábia Guimarães
Borges, Anna C. Neves
Conforte, Alessandra J.
Kampmann, Leonardo Giovanella
Ferreira, André V. J.
Amorim, Regina M. S.
Benevent, Magda A.
Sá, Maria Eugênia Lisei de
Mesquita, Rosilene O.
Rodrigues, Fabiana A.
Nepomuceno, Alexandre L.
Romano, Eduardo
Sá, Maria Fátima Grossi de
Ferreira, Márcio Alves
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Loureiro, Marcelo E.
Dias, Fábia Guimarães
Borges, Anna C. Neves
Conforte, Alessandra J.
Kampmann, Leonardo Giovanella
Ferreira, André V. J.
Amorim, Regina M. S.
Benevent, Magda A.
Sá, Maria Eugênia Lisei de
Mesquita, Rosilene O.
Rodrigues, Fabiana A.
Nepomuceno, Alexandre L.
Romano, Eduardo
Sá, Maria Fátima Grossi de
Ferreira, Márcio Alves
dc.subject.pt-BR.fl_str_mv Drought adaptation
Glycine max
Marker assistance selection
Transcription factors
Water deficit
topic Drought adaptation
Glycine max
Marker assistance selection
Transcription factors
Water deficit
description Soybean (Glycine max) is one of the major world commodities. In order to increase the soybean yields, it has been searched drought-tolerant cultivars, once the drought is the major constraint to soybean grown. Therefore, it is crucial to elucidate the molecular mechanisms associated with drought tolerance. Here, the in silico approach allowed us to identify 12 genes belonging to six different transcription factor families in soybean that have been associated with key events on drought response. The expression pattern of each gene was investigated by qPCR in root samples of drought-sensitive and drought-tolerant cultivars undergoing drought stress in pot-based (PSys) and hydroponic (HSys) cultivation systems. GmaxMYC2-like 2 was induced under abrupt drought conditions in HSys in both cultivars, whereas GmaxAREB1-like 1 and GmaxDREB2A-like were highly induced only in the PSys. However, GmaxMYB2-like 1, GmaxMYB2-like 2, GmaxRD26/NAC-like 1, GmaxRD26/NAC-like 2, GmaxAREB1-like 2, and GmaxDREB1A-like were upregulated in both systems, while the GmaxHB6-like and GmaxHB13-like are repressed under all of the investigated conditions. Exogenous abscisic acid (ABA) treatment was used to identify those genes belonging to the ABA-dependent drought response. The genes identified in this work have potential application for the improvement of drought resistance in soybean and markers for breeding programs.
publishDate 2016
dc.date.issued.fl_str_mv 2016-12
dc.date.accessioned.fl_str_mv 2018-09-25T18:17:02Z
dc.date.available.fl_str_mv 2018-09-25T18:17:02Z
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dc.identifier.uri.fl_str_mv https://doi.org/10.1007/s11105-016-0993-z
http://www.locus.ufv.br/handle/123456789/21998
dc.identifier.issn.none.fl_str_mv 1572-9818
identifier_str_mv 1572-9818
url https://doi.org/10.1007/s11105-016-0993-z
http://www.locus.ufv.br/handle/123456789/21998
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 34, Issue 6, p. 1167–1180, December 2016
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