High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer

Detalhes bibliográficos
Autor(a) principal: Panov,Sasho
Data de Publicação: 2004
Outros Autores: Roganovic-Zafirova,Danica, Stavric,George, Yashar,Genghis, Popov,Zivko
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100002
Resumo: Point mutations at codon 12 of the HRAS (v-Ha-ras Harvey rat sarcoma viral oncogene homolog) oncogene are one of the best defined and widely studied molecular genetic events in transitional cell carcinoma (TCC) of the urinary bladder. The aim of this study was to use the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of paraffin-embedded tissue-derived DNA to determine the frequency of the HRAS oncogene G ->T codon 12 mutation in TCC patients being treated at the University Urology Clinic in Skopje, Republic of Macedonia. DNA isolated from paraffin-embedded tissue (PET) surgically removed TCC specimens of 62 (81.58%) out of 76 patients were successfully amplified, the remaining 14 (18.42%) showing compromised DNA integrity. The codon 12 mutation of the HRAS oncogene was found in 24 (38.71%) out of 62 successfully tested TCC urinary bladder samples. No significant relationship between the mutation frequency and the histopathological grade of tumor differentiation was detected (chi² = 0.044; p = 0.978). The relatively high frequency of mutations found in our study was comparable with some of the previously reported data obtained by this and/or other PCR-based methods. This highly sensitive and specific PCR-RFLP analysis was demonstrated to be a suitable method for the detection of mutations at codon 12 of the HRAS oncogene in PET samples of urinary bladder TCC.
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spelling High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancerHRASoncogenebladder cancerPCR-RFLPPoint mutations at codon 12 of the HRAS (v-Ha-ras Harvey rat sarcoma viral oncogene homolog) oncogene are one of the best defined and widely studied molecular genetic events in transitional cell carcinoma (TCC) of the urinary bladder. The aim of this study was to use the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of paraffin-embedded tissue-derived DNA to determine the frequency of the HRAS oncogene G ->T codon 12 mutation in TCC patients being treated at the University Urology Clinic in Skopje, Republic of Macedonia. DNA isolated from paraffin-embedded tissue (PET) surgically removed TCC specimens of 62 (81.58%) out of 76 patients were successfully amplified, the remaining 14 (18.42%) showing compromised DNA integrity. The codon 12 mutation of the HRAS oncogene was found in 24 (38.71%) out of 62 successfully tested TCC urinary bladder samples. No significant relationship between the mutation frequency and the histopathological grade of tumor differentiation was detected (chi² = 0.044; p = 0.978). The relatively high frequency of mutations found in our study was comparable with some of the previously reported data obtained by this and/or other PCR-based methods. This highly sensitive and specific PCR-RFLP analysis was demonstrated to be a suitable method for the detection of mutations at codon 12 of the HRAS oncogene in PET samples of urinary bladder TCC.Sociedade Brasileira de Genética2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100002Genetics and Molecular Biology v.27 n.1 2004reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572004000100002info:eu-repo/semantics/openAccessPanov,SashoRoganovic-Zafirova,DanicaStavric,GeorgeYashar,GenghisPopov,Zivkoeng2004-04-14T00:00:00Zoai:scielo:S1415-47572004000100002Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2004-04-14T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
title High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
spellingShingle High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
Panov,Sasho
HRAS
oncogene
bladder cancer
PCR-RFLP
title_short High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
title_full High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
title_fullStr High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
title_full_unstemmed High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
title_sort High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer
author Panov,Sasho
author_facet Panov,Sasho
Roganovic-Zafirova,Danica
Stavric,George
Yashar,Genghis
Popov,Zivko
author_role author
author2 Roganovic-Zafirova,Danica
Stavric,George
Yashar,Genghis
Popov,Zivko
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Panov,Sasho
Roganovic-Zafirova,Danica
Stavric,George
Yashar,Genghis
Popov,Zivko
dc.subject.por.fl_str_mv HRAS
oncogene
bladder cancer
PCR-RFLP
topic HRAS
oncogene
bladder cancer
PCR-RFLP
description Point mutations at codon 12 of the HRAS (v-Ha-ras Harvey rat sarcoma viral oncogene homolog) oncogene are one of the best defined and widely studied molecular genetic events in transitional cell carcinoma (TCC) of the urinary bladder. The aim of this study was to use the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of paraffin-embedded tissue-derived DNA to determine the frequency of the HRAS oncogene G ->T codon 12 mutation in TCC patients being treated at the University Urology Clinic in Skopje, Republic of Macedonia. DNA isolated from paraffin-embedded tissue (PET) surgically removed TCC specimens of 62 (81.58%) out of 76 patients were successfully amplified, the remaining 14 (18.42%) showing compromised DNA integrity. The codon 12 mutation of the HRAS oncogene was found in 24 (38.71%) out of 62 successfully tested TCC urinary bladder samples. No significant relationship between the mutation frequency and the histopathological grade of tumor differentiation was detected (chi² = 0.044; p = 0.978). The relatively high frequency of mutations found in our study was comparable with some of the previously reported data obtained by this and/or other PCR-based methods. This highly sensitive and specific PCR-RFLP analysis was demonstrated to be a suitable method for the detection of mutations at codon 12 of the HRAS oncogene in PET samples of urinary bladder TCC.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572004000100002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.27 n.1 2004
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
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instname_str Sociedade Brasileira de Genética (SBG)
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collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
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