Detection of codon 12 mutation in the k-ras oncogene in pancreatic tumors

• Autor(a) principal: Kubrusly,Márcia Saldanha
• Data de Publicação: 1999
• Outros Autores: Matheucci Junior,Euclides, Leite,Kátia Ramos Moreira, Coelho,Ana Maria de Mendonça, Monte,Osmar, Machado,Marcel Cerqueira Cesar, Pinotti,Henrique Walter
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista do Hospital das Clínicas
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87811999000100004
• topic: Point mutation; Oncogene K-ras; Pancreatic adenocarcinoma; Polymerase chain reaction (PCR)

Mutations at codons 12, 13, or 61 of the H-ras, K-ras, and N-ras have been detected in human neoplasias by a variety of techniques. Some of these techniques are very sensitive and can detect K-ras mutation in 90% of the cases of pancreatic adenocarcinomas. We analyzed 11 samples of pancreatic adenocarcinoma, three samples of pancreatic mucinous cystadenoma, and two samples without tumors in formalin-fixed paraffin embedded tissue sections. K-ras mutations at codon 12 were detected by a two-step PCR-enriched technique in all the samples of pancreatic adenocarcinoma, but not in cystadenoma or control samples. This technique may be useful for early detection of pancreatic cancer.