DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients

Detalhes bibliográficos
Autor(a) principal: Lima,Patrícia Santos Pereira
Data de Publicação: 2008
Outros Autores: Molffeta,Greice Andreoti, Araujo,Amélia Góes de, Zago,Marco Antônio, Silva Jr.,Wilson Araújo da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005
Resumo: The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients.
id SBG-1_3ef6d7043dfb88449af018252707d759
oai_identifier_str oai:scielo:S1415-47572008000400005
network_acronym_str SBG-1
network_name_str Genetics and Molecular Biology
repository_id_str
spelling DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patientsCpG islandDNA methylationCDKN2BThe aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients.Sociedade Brasileira de Genética2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005Genetics and Molecular Biology v.31 n.3 2008reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572008000400005info:eu-repo/semantics/openAccessLima,Patrícia Santos PereiraMolffeta,Greice AndreotiAraujo,Amélia Góes deZago,Marco AntônioSilva Jr.,Wilson Araújo daeng2008-08-18T00:00:00Zoai:scielo:S1415-47572008000400005Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2008-08-18T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
title DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
spellingShingle DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
Lima,Patrícia Santos Pereira
CpG island
DNA methylation
CDKN2B
title_short DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
title_full DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
title_fullStr DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
title_full_unstemmed DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
title_sort DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
author Lima,Patrícia Santos Pereira
author_facet Lima,Patrícia Santos Pereira
Molffeta,Greice Andreoti
Araujo,Amélia Góes de
Zago,Marco Antônio
Silva Jr.,Wilson Araújo da
author_role author
author2 Molffeta,Greice Andreoti
Araujo,Amélia Góes de
Zago,Marco Antônio
Silva Jr.,Wilson Araújo da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lima,Patrícia Santos Pereira
Molffeta,Greice Andreoti
Araujo,Amélia Góes de
Zago,Marco Antônio
Silva Jr.,Wilson Araújo da
dc.subject.por.fl_str_mv CpG island
DNA methylation
CDKN2B
topic CpG island
DNA methylation
CDKN2B
description The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572008000400005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.31 n.3 2008
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
_version_ 1752122381446414336

Registros relacionados