Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer

Detalhes bibliográficos
Autor(a) principal: LIMA, Eleonidas Moura
Data de Publicação: 2008
Outros Autores: LEAL, Mariana Ferreira, BURBANO, Rommel Mario Rodriguéz, KHAYAT, André Salim, ASSUMPÇÃO, Paulo Pimentel de, BELLO, Maria Josefa, HERRANZ, Juan Antonio Rey, SMITH, Marília de Arruda Cardoso, CASARTELLI, Cacilda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFPA
Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/3439
Resumo: Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA extraction. Patients had never been submitted to chemotherapy or radiotherapy, nor did they have any other diagnosed cancer. None of the gastric cancer samples presented methylated DNA sequences for ANAPC1 and TP53. CDKN2A methylation was not detected in any normal gastric mucosa; however, the CDKN2A promoter was methylated in 30.4% of gastric cancer samples, with 35% methylation in diffuse-type and 26.9% in intestinal-type cancers. CDKN2A methylation was associated with the carcinogenesis process for ~30% diffuse-type and intestinal-type compared to non-neoplastic samples. Thus, ANAPC1 and TP53 methylation was probably not implicated in gastric carcinogenesis in our samples. CDKN2A can be implicated in the carcinogenesis process of only a subset of gastric neoplasias.
id UFPA_066e28c97d24ce44a11e744366cdd74f
oai_identifier_str oai:repositorio.ufpa.br:2011/3439
network_acronym_str UFPA
network_name_str Repositório Institucional da UFPA
repository_id_str 2123
spelling 2013-02-05T13:29:23Z2013-02-05T13:29:23Z2008-06LIMA, E. M. et al. Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer. Brazilian Journal of Medical and Biological Research. Ribeirão Preto, v. 41, n. 6, p. 539-543, jun. 2008. Disponível em: <http://www.scielo.br/pdf/bjmbr/v41n6/7136.pdf>. Acesso em: 31 jan. 2013. <http://dx.doi.org/10.1590/S0100-879X2008000600017>.0100-879Xhttp://repositorio.ufpa.br/jspui/handle/2011/3439Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA extraction. Patients had never been submitted to chemotherapy or radiotherapy, nor did they have any other diagnosed cancer. None of the gastric cancer samples presented methylated DNA sequences for ANAPC1 and TP53. CDKN2A methylation was not detected in any normal gastric mucosa; however, the CDKN2A promoter was methylated in 30.4% of gastric cancer samples, with 35% methylation in diffuse-type and 26.9% in intestinal-type cancers. CDKN2A methylation was associated with the carcinogenesis process for ~30% diffuse-type and intestinal-type compared to non-neoplastic samples. Thus, ANAPC1 and TP53 methylation was probably not implicated in gastric carcinogenesis in our samples. CDKN2A can be implicated in the carcinogenesis process of only a subset of gastric neoplasias.Submitted by Samira Prince (prince@ufpa.br) on 2013-01-31T13:58:22Z No. of bitstreams: 2 license_rdf: 23599 bytes, checksum: 9e2b7f6edbd693264102b96ece20428a (MD5) Artigo_MethylationStatusANAPC1.pdf: 509966 bytes, checksum: 78668c07849f3d8720fa73e92cfe9197 (MD5)Approved for entry into archive by Ana Rosa Silva(arosa@ufpa.br) on 2013-02-05T13:29:23Z (GMT) No. of bitstreams: 2 license_rdf: 23599 bytes, checksum: 9e2b7f6edbd693264102b96ece20428a (MD5) Artigo_MethylationStatusANAPC1.pdf: 509966 bytes, checksum: 78668c07849f3d8720fa73e92cfe9197 (MD5)Made available in DSpace on 2013-02-05T13:29:23Z (GMT). No. of bitstreams: 2 license_rdf: 23599 bytes, checksum: 9e2b7f6edbd693264102b96ece20428a (MD5) Artigo_MethylationStatusANAPC1.pdf: 509966 bytes, checksum: 78668c07849f3d8720fa73e92cfe9197 (MD5) Previous issue date: 2008-06engMetilação de DNANeoplasias gástricasANAPC1CDKN2ATP53Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLIMA, Eleonidas MouraLEAL, Mariana FerreiraBURBANO, Rommel Mario RodriguézKHAYAT, André SalimASSUMPÇÃO, Paulo Pimentel deBELLO, Maria JosefaHERRANZ, Juan Antonio ReySMITH, Marília de Arruda CardosoCASARTELLI, Cacildainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_MethylationStatusANAPC1.pdfArtigo_MethylationStatusANAPC1.pdfapplication/pdf509966https://repositorio.ufpa.br/oai/bitstream/2011/3439/1/Artigo_MethylationStatusANAPC1.pdf78668c07849f3d8720fa73e92cfe9197MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849https://repositorio.ufpa.br/oai/bitstream/2011/3439/2/license_urlfd26723f8d7edacdb29e3f03465c3b03MD52license_textlicense_texttext/html; charset=utf-80https://repositorio.ufpa.br/oai/bitstream/2011/3439/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823599https://repositorio.ufpa.br/oai/bitstream/2011/3439/4/license_rdf9e2b7f6edbd693264102b96ece20428aMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81996https://repositorio.ufpa.br/oai/bitstream/2011/3439/5/license.txt4b350777eea7a4b1ae7cd9a06fd687a1MD55TEXTArtigo_MethylationStatusANAPC1.pdf.txtArtigo_MethylationStatusANAPC1.pdf.txtExtracted texttext/plain19507https://repositorio.ufpa.br/oai/bitstream/2011/3439/6/Artigo_MethylationStatusANAPC1.pdf.txt8b340c8ec8a5067dc05f1871954766deMD562011/34392024-09-18 11:20:49.877oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232024-09-18T14:20:49Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false
dc.title.pt_BR.fl_str_mv Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
title Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
spellingShingle Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
LIMA, Eleonidas Moura
Metilação de DNA
Neoplasias gástricas
ANAPC1
CDKN2A
TP53
title_short Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
title_full Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
title_fullStr Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
title_full_unstemmed Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
title_sort Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
author LIMA, Eleonidas Moura
author_facet LIMA, Eleonidas Moura
LEAL, Mariana Ferreira
BURBANO, Rommel Mario Rodriguéz
KHAYAT, André Salim
ASSUMPÇÃO, Paulo Pimentel de
BELLO, Maria Josefa
HERRANZ, Juan Antonio Rey
SMITH, Marília de Arruda Cardoso
CASARTELLI, Cacilda
author_role author
author2 LEAL, Mariana Ferreira
BURBANO, Rommel Mario Rodriguéz
KHAYAT, André Salim
ASSUMPÇÃO, Paulo Pimentel de
BELLO, Maria Josefa
HERRANZ, Juan Antonio Rey
SMITH, Marília de Arruda Cardoso
CASARTELLI, Cacilda
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv LIMA, Eleonidas Moura
LEAL, Mariana Ferreira
BURBANO, Rommel Mario Rodriguéz
KHAYAT, André Salim
ASSUMPÇÃO, Paulo Pimentel de
BELLO, Maria Josefa
HERRANZ, Juan Antonio Rey
SMITH, Marília de Arruda Cardoso
CASARTELLI, Cacilda
dc.subject.por.fl_str_mv Metilação de DNA
Neoplasias gástricas
ANAPC1
CDKN2A
TP53
topic Metilação de DNA
Neoplasias gástricas
ANAPC1
CDKN2A
TP53
description Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA extraction. Patients had never been submitted to chemotherapy or radiotherapy, nor did they have any other diagnosed cancer. None of the gastric cancer samples presented methylated DNA sequences for ANAPC1 and TP53. CDKN2A methylation was not detected in any normal gastric mucosa; however, the CDKN2A promoter was methylated in 30.4% of gastric cancer samples, with 35% methylation in diffuse-type and 26.9% in intestinal-type cancers. CDKN2A methylation was associated with the carcinogenesis process for ~30% diffuse-type and intestinal-type compared to non-neoplastic samples. Thus, ANAPC1 and TP53 methylation was probably not implicated in gastric carcinogenesis in our samples. CDKN2A can be implicated in the carcinogenesis process of only a subset of gastric neoplasias.
publishDate 2008
dc.date.issued.fl_str_mv 2008-06
dc.date.accessioned.fl_str_mv 2013-02-05T13:29:23Z
dc.date.available.fl_str_mv 2013-02-05T13:29:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, E. M. et al. Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer. Brazilian Journal of Medical and Biological Research. Ribeirão Preto, v. 41, n. 6, p. 539-543, jun. 2008. Disponível em: <http://www.scielo.br/pdf/bjmbr/v41n6/7136.pdf>. Acesso em: 31 jan. 2013. <http://dx.doi.org/10.1590/S0100-879X2008000600017>.
dc.identifier.uri.fl_str_mv http://repositorio.ufpa.br/jspui/handle/2011/3439
dc.identifier.issn.none.fl_str_mv 0100-879X
identifier_str_mv LIMA, E. M. et al. Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer. Brazilian Journal of Medical and Biological Research. Ribeirão Preto, v. 41, n. 6, p. 539-543, jun. 2008. Disponível em: <http://www.scielo.br/pdf/bjmbr/v41n6/7136.pdf>. Acesso em: 31 jan. 2013. <http://dx.doi.org/10.1590/S0100-879X2008000600017>.
0100-879X
url http://repositorio.ufpa.br/jspui/handle/2011/3439
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFPA
instname:Universidade Federal do Pará (UFPA)
instacron:UFPA
instname_str Universidade Federal do Pará (UFPA)
instacron_str UFPA
institution UFPA
reponame_str Repositório Institucional da UFPA
collection Repositório Institucional da UFPA
bitstream.url.fl_str_mv https://repositorio.ufpa.br/oai/bitstream/2011/3439/1/Artigo_MethylationStatusANAPC1.pdf
https://repositorio.ufpa.br/oai/bitstream/2011/3439/2/license_url
https://repositorio.ufpa.br/oai/bitstream/2011/3439/3/license_text
https://repositorio.ufpa.br/oai/bitstream/2011/3439/4/license_rdf
https://repositorio.ufpa.br/oai/bitstream/2011/3439/5/license.txt
https://repositorio.ufpa.br/oai/bitstream/2011/3439/6/Artigo_MethylationStatusANAPC1.pdf.txt
bitstream.checksum.fl_str_mv 78668c07849f3d8720fa73e92cfe9197
fd26723f8d7edacdb29e3f03465c3b03
d41d8cd98f00b204e9800998ecf8427e
9e2b7f6edbd693264102b96ece20428a
4b350777eea7a4b1ae7cd9a06fd687a1
8b340c8ec8a5067dc05f1871954766de
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)
repository.mail.fl_str_mv riufpabc@ufpa.br
_version_ 1813552054373711872