Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/3439 |
Resumo: | Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA extraction. Patients had never been submitted to chemotherapy or radiotherapy, nor did they have any other diagnosed cancer. None of the gastric cancer samples presented methylated DNA sequences for ANAPC1 and TP53. CDKN2A methylation was not detected in any normal gastric mucosa; however, the CDKN2A promoter was methylated in 30.4% of gastric cancer samples, with 35% methylation in diffuse-type and 26.9% in intestinal-type cancers. CDKN2A methylation was associated with the carcinogenesis process for ~30% diffuse-type and intestinal-type compared to non-neoplastic samples. Thus, ANAPC1 and TP53 methylation was probably not implicated in gastric carcinogenesis in our samples. CDKN2A can be implicated in the carcinogenesis process of only a subset of gastric neoplasias. |
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2013-02-05T13:29:23Z2013-02-05T13:29:23Z2008-06LIMA, E. M. et al. Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer. Brazilian Journal of Medical and Biological Research. Ribeirão Preto, v. 41, n. 6, p. 539-543, jun. 2008. Disponível em: <http://www.scielo.br/pdf/bjmbr/v41n6/7136.pdf>. Acesso em: 31 jan. 2013. <http://dx.doi.org/10.1590/S0100-879X2008000600017>.0100-879Xhttp://repositorio.ufpa.br/jspui/handle/2011/3439Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA extraction. Patients had never been submitted to chemotherapy or radiotherapy, nor did they have any other diagnosed cancer. None of the gastric cancer samples presented methylated DNA sequences for ANAPC1 and TP53. CDKN2A methylation was not detected in any normal gastric mucosa; however, the CDKN2A promoter was methylated in 30.4% of gastric cancer samples, with 35% methylation in diffuse-type and 26.9% in intestinal-type cancers. CDKN2A methylation was associated with the carcinogenesis process for ~30% diffuse-type and intestinal-type compared to non-neoplastic samples. Thus, ANAPC1 and TP53 methylation was probably not implicated in gastric carcinogenesis in our samples. CDKN2A can be implicated in the carcinogenesis process of only a subset of gastric neoplasias.Submitted by Samira Prince (prince@ufpa.br) on 2013-01-31T13:58:22Z No. of bitstreams: 2 license_rdf: 23599 bytes, checksum: 9e2b7f6edbd693264102b96ece20428a (MD5) Artigo_MethylationStatusANAPC1.pdf: 509966 bytes, checksum: 78668c07849f3d8720fa73e92cfe9197 (MD5)Approved for entry into archive by Ana Rosa Silva(arosa@ufpa.br) on 2013-02-05T13:29:23Z (GMT) No. of bitstreams: 2 license_rdf: 23599 bytes, checksum: 9e2b7f6edbd693264102b96ece20428a (MD5) Artigo_MethylationStatusANAPC1.pdf: 509966 bytes, checksum: 78668c07849f3d8720fa73e92cfe9197 (MD5)Made available in DSpace on 2013-02-05T13:29:23Z (GMT). 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dc.title.pt_BR.fl_str_mv |
Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer |
title |
Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer |
spellingShingle |
Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer LIMA, Eleonidas Moura Metilação de DNA Neoplasias gástricas ANAPC1 CDKN2A TP53 |
title_short |
Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer |
title_full |
Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer |
title_fullStr |
Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer |
title_full_unstemmed |
Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer |
title_sort |
Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer |
author |
LIMA, Eleonidas Moura |
author_facet |
LIMA, Eleonidas Moura LEAL, Mariana Ferreira BURBANO, Rommel Mario Rodriguéz KHAYAT, André Salim ASSUMPÇÃO, Paulo Pimentel de BELLO, Maria Josefa HERRANZ, Juan Antonio Rey SMITH, Marília de Arruda Cardoso CASARTELLI, Cacilda |
author_role |
author |
author2 |
LEAL, Mariana Ferreira BURBANO, Rommel Mario Rodriguéz KHAYAT, André Salim ASSUMPÇÃO, Paulo Pimentel de BELLO, Maria Josefa HERRANZ, Juan Antonio Rey SMITH, Marília de Arruda Cardoso CASARTELLI, Cacilda |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
LIMA, Eleonidas Moura LEAL, Mariana Ferreira BURBANO, Rommel Mario Rodriguéz KHAYAT, André Salim ASSUMPÇÃO, Paulo Pimentel de BELLO, Maria Josefa HERRANZ, Juan Antonio Rey SMITH, Marília de Arruda Cardoso CASARTELLI, Cacilda |
dc.subject.por.fl_str_mv |
Metilação de DNA Neoplasias gástricas ANAPC1 CDKN2A TP53 |
topic |
Metilação de DNA Neoplasias gástricas ANAPC1 CDKN2A TP53 |
description |
Gastric cancer is the forth most frequent malignancy and the second most common cause of cancer death worldwide. DNA methylation is the most studied epigenetic alteration, occurring through a methyl radical addition to the cytosine base adjacent to guanine. Many tumor genes are inactivated by DNA methylation in gastric cancer. We evaluated the DNA methylation status of ANAPC1, CDKN2A and TP53 by methylation-specific PCR in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosa in individuals from Northern Brazil. All gastric cancer samples were advanced stage adenocarcinomas. Gastric samples were surgically obtained at the João de Barros Barreto University Hospital, State of Pará, and were stored at -80°C before DNA extraction. Patients had never been submitted to chemotherapy or radiotherapy, nor did they have any other diagnosed cancer. None of the gastric cancer samples presented methylated DNA sequences for ANAPC1 and TP53. CDKN2A methylation was not detected in any normal gastric mucosa; however, the CDKN2A promoter was methylated in 30.4% of gastric cancer samples, with 35% methylation in diffuse-type and 26.9% in intestinal-type cancers. CDKN2A methylation was associated with the carcinogenesis process for ~30% diffuse-type and intestinal-type compared to non-neoplastic samples. Thus, ANAPC1 and TP53 methylation was probably not implicated in gastric carcinogenesis in our samples. CDKN2A can be implicated in the carcinogenesis process of only a subset of gastric neoplasias. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-06 |
dc.date.accessioned.fl_str_mv |
2013-02-05T13:29:23Z |
dc.date.available.fl_str_mv |
2013-02-05T13:29:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, E. M. et al. Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer. Brazilian Journal of Medical and Biological Research. Ribeirão Preto, v. 41, n. 6, p. 539-543, jun. 2008. Disponível em: <http://www.scielo.br/pdf/bjmbr/v41n6/7136.pdf>. Acesso em: 31 jan. 2013. <http://dx.doi.org/10.1590/S0100-879X2008000600017>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/3439 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
identifier_str_mv |
LIMA, E. M. et al. Methylation status of ANAPC1, CDKN2A and TP53 promoter genes in individuals with gastric cancer. Brazilian Journal of Medical and Biological Research. Ribeirão Preto, v. 41, n. 6, p. 539-543, jun. 2008. Disponível em: <http://www.scielo.br/pdf/bjmbr/v41n6/7136.pdf>. Acesso em: 31 jan. 2013. <http://dx.doi.org/10.1590/S0100-879X2008000600017>. 0100-879X |
url |
http://repositorio.ufpa.br/jspui/handle/2011/3439 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Universidade Federal do Pará (UFPA) |
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UFPA |
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UFPA |
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