Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200024 |
Resumo: | In this study, we used real-time quantitative PCR (RT-qPCR) to evaluate the expression of 32 genes of Xanthomonas axonopodis pv. citri related to pathogenicity and virulence that are also involved in copper detoxification. Nearly all of the genes were up-regulated, including copA and copB. Two genes homologous to members of the type II secretion system (xcsH and xcsC) and two involved in the degradation of plant cell wall components (pglA and pel) were the most expressed in response to an elevated copper concentration. The type II secretion system (xcs operon) and a few homologues of proteins putatively secreted by this system showed enhanced expression when the bacteria were exposed to a high concentration of copper sulfate. The enhanced expression of the genes of secretion II system during copper stress suggests that this pathway may have an important role in the adaptative response of X. axonopodis pv. citri to toxic compounds. These findings highlight the potential role of these genes in attenuating the toxicity of certain metals and could represent an important means of bacterial resistance against chemicals used to control diseases. |
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Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stresscopper resistancecitrus cankerreal-time quantitative PCRIn this study, we used real-time quantitative PCR (RT-qPCR) to evaluate the expression of 32 genes of Xanthomonas axonopodis pv. citri related to pathogenicity and virulence that are also involved in copper detoxification. Nearly all of the genes were up-regulated, including copA and copB. Two genes homologous to members of the type II secretion system (xcsH and xcsC) and two involved in the degradation of plant cell wall components (pglA and pel) were the most expressed in response to an elevated copper concentration. The type II secretion system (xcs operon) and a few homologues of proteins putatively secreted by this system showed enhanced expression when the bacteria were exposed to a high concentration of copper sulfate. The enhanced expression of the genes of secretion II system during copper stress suggests that this pathway may have an important role in the adaptative response of X. axonopodis pv. citri to toxic compounds. These findings highlight the potential role of these genes in attenuating the toxicity of certain metals and could represent an important means of bacterial resistance against chemicals used to control diseases.Sociedade Brasileira de Genética2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200024Genetics and Molecular Biology v.33 n.2 2010reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572010005000030info:eu-repo/semantics/openAccessPalmieri,Ana Carolina BasílioAmaral,Alexandre Morais doHomem,Rafael AugustoMachado,Marcos Antonioeng2010-06-14T00:00:00Zoai:scielo:S1415-47572010000200024Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2010-06-14T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress |
title |
Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress |
spellingShingle |
Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress Palmieri,Ana Carolina Basílio copper resistance citrus canker real-time quantitative PCR |
title_short |
Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress |
title_full |
Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress |
title_fullStr |
Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress |
title_full_unstemmed |
Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress |
title_sort |
Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress |
author |
Palmieri,Ana Carolina Basílio |
author_facet |
Palmieri,Ana Carolina Basílio Amaral,Alexandre Morais do Homem,Rafael Augusto Machado,Marcos Antonio |
author_role |
author |
author2 |
Amaral,Alexandre Morais do Homem,Rafael Augusto Machado,Marcos Antonio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Palmieri,Ana Carolina Basílio Amaral,Alexandre Morais do Homem,Rafael Augusto Machado,Marcos Antonio |
dc.subject.por.fl_str_mv |
copper resistance citrus canker real-time quantitative PCR |
topic |
copper resistance citrus canker real-time quantitative PCR |
description |
In this study, we used real-time quantitative PCR (RT-qPCR) to evaluate the expression of 32 genes of Xanthomonas axonopodis pv. citri related to pathogenicity and virulence that are also involved in copper detoxification. Nearly all of the genes were up-regulated, including copA and copB. Two genes homologous to members of the type II secretion system (xcsH and xcsC) and two involved in the degradation of plant cell wall components (pglA and pel) were the most expressed in response to an elevated copper concentration. The type II secretion system (xcs operon) and a few homologues of proteins putatively secreted by this system showed enhanced expression when the bacteria were exposed to a high concentration of copper sulfate. The enhanced expression of the genes of secretion II system during copper stress suggests that this pathway may have an important role in the adaptative response of X. axonopodis pv. citri to toxic compounds. These findings highlight the potential role of these genes in attenuating the toxicity of certain metals and could represent an important means of bacterial resistance against chemicals used to control diseases. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200024 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572010005000030 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.33 n.2 2010 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122383241576448 |