Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200003 |
Resumo: | We report a sampling strategy based on Mendelian Breeding Units (MBUs), representing an interbreeding group of individuals sharing a common gene pool. The identification of MBUs is crucial for case-control experimental design in association studies. The aim of this work was to evaluate the possible existence of bias in terms of genetic variability and haplogroup frequencies in the MBU sample, due to severe sample selection. In order to reach this goal, the MBU sampling strategy was compared to a standard selection of individuals according to their surname and place of birth. We analysed mitochondrial DNA variation (first hypervariable segment and coding region) in unrelated healthy subjects from two different areas of Sardinia: the area around the town of Cabras and the western Campidano area. No statistically significant differences were observed when the two sampling methods were compared, indicating that the stringent sample selection needed to establish a MBU does not alter original genetic variability and haplogroup distribution. Therefore, the MBU sampling strategy can be considered a useful tool in association studies of complex traits. |
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Genetics and Molecular Biology |
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Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardiniabreeding units strategymtDNA haplogroup distributionassociation studiesWe report a sampling strategy based on Mendelian Breeding Units (MBUs), representing an interbreeding group of individuals sharing a common gene pool. The identification of MBUs is crucial for case-control experimental design in association studies. The aim of this work was to evaluate the possible existence of bias in terms of genetic variability and haplogroup frequencies in the MBU sample, due to severe sample selection. In order to reach this goal, the MBU sampling strategy was compared to a standard selection of individuals according to their surname and place of birth. We analysed mitochondrial DNA variation (first hypervariable segment and coding region) in unrelated healthy subjects from two different areas of Sardinia: the area around the town of Cabras and the western Campidano area. No statistically significant differences were observed when the two sampling methods were compared, indicating that the stringent sample selection needed to establish a MBU does not alter original genetic variability and haplogroup distribution. Therefore, the MBU sampling strategy can be considered a useful tool in association studies of complex traits.Sociedade Brasileira de Genética2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200003Genetics and Molecular Biology v.34 n.2 2011reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572011000200003info:eu-repo/semantics/openAccessSanna,DariaPala,MariaCossu,PieroDedola,Gian LucaMelis,SoniaFresu,GiovanniMorelli,LauraObinu,DomenicaTonolo,GiancarloSecchi,GianninaTriunfo,RiccardoLorenz,Joseph G.Scheinfeldt,LauraTorroni,AntonioRobledo,RenatoFrancalacci,Paoloeng2011-06-02T00:00:00Zoai:scielo:S1415-47572011000200003Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2011-06-02T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia |
title |
Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia |
spellingShingle |
Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia Sanna,Daria breeding units strategy mtDNA haplogroup distribution association studies |
title_short |
Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia |
title_full |
Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia |
title_fullStr |
Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia |
title_full_unstemmed |
Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia |
title_sort |
Mendelian breeding units versus standard sampling strategies: mitochondrial DNA variation in southwest Sardinia |
author |
Sanna,Daria |
author_facet |
Sanna,Daria Pala,Maria Cossu,Piero Dedola,Gian Luca Melis,Sonia Fresu,Giovanni Morelli,Laura Obinu,Domenica Tonolo,Giancarlo Secchi,Giannina Triunfo,Riccardo Lorenz,Joseph G. Scheinfeldt,Laura Torroni,Antonio Robledo,Renato Francalacci,Paolo |
author_role |
author |
author2 |
Pala,Maria Cossu,Piero Dedola,Gian Luca Melis,Sonia Fresu,Giovanni Morelli,Laura Obinu,Domenica Tonolo,Giancarlo Secchi,Giannina Triunfo,Riccardo Lorenz,Joseph G. Scheinfeldt,Laura Torroni,Antonio Robledo,Renato Francalacci,Paolo |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sanna,Daria Pala,Maria Cossu,Piero Dedola,Gian Luca Melis,Sonia Fresu,Giovanni Morelli,Laura Obinu,Domenica Tonolo,Giancarlo Secchi,Giannina Triunfo,Riccardo Lorenz,Joseph G. Scheinfeldt,Laura Torroni,Antonio Robledo,Renato Francalacci,Paolo |
dc.subject.por.fl_str_mv |
breeding units strategy mtDNA haplogroup distribution association studies |
topic |
breeding units strategy mtDNA haplogroup distribution association studies |
description |
We report a sampling strategy based on Mendelian Breeding Units (MBUs), representing an interbreeding group of individuals sharing a common gene pool. The identification of MBUs is crucial for case-control experimental design in association studies. The aim of this work was to evaluate the possible existence of bias in terms of genetic variability and haplogroup frequencies in the MBU sample, due to severe sample selection. In order to reach this goal, the MBU sampling strategy was compared to a standard selection of individuals according to their surname and place of birth. We analysed mitochondrial DNA variation (first hypervariable segment and coding region) in unrelated healthy subjects from two different areas of Sardinia: the area around the town of Cabras and the western Campidano area. No statistically significant differences were observed when the two sampling methods were compared, indicating that the stringent sample selection needed to establish a MBU does not alter original genetic variability and haplogroup distribution. Therefore, the MBU sampling strategy can be considered a useful tool in association studies of complex traits. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572011000200003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.34 n.2 2011 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122383700852736 |