In situ DNA transfer to chicken embryos by biolistics
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400010 |
Resumo: | Fertilized chicken eggs were bombarded with a biolistic device. Transient expression of the lacZ gene under the control of a human cytomegalovirus (CMV) promoter was assessed after in situ gene transfer using this approach. The influence of different pressures, vacuum levels and particles was tested. Survival rate improved as particle velocity decreased, but resulted in lower levels of expression. The best survival and expression were obtained with gold particles, a helium gas pressure of 600 psi and a vacuum of 600 mmHg. Under these conditions, all bombarded embryos showed <FONT FACE="Symbol">b</FONT>-galactosidase activity, indicating that this was an effective method for transformation of chicken embryos. |
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Genetics and Molecular Biology |
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In situ DNA transfer to chicken embryos by biolisticsFertilized chicken eggs were bombarded with a biolistic device. Transient expression of the lacZ gene under the control of a human cytomegalovirus (CMV) promoter was assessed after in situ gene transfer using this approach. The influence of different pressures, vacuum levels and particles was tested. Survival rate improved as particle velocity decreased, but resulted in lower levels of expression. The best survival and expression were obtained with gold particles, a helium gas pressure of 600 psi and a vacuum of 600 mmHg. Under these conditions, all bombarded embryos showed <FONT FACE="Symbol">b</FONT>-galactosidase activity, indicating that this was an effective method for transformation of chicken embryos.Sociedade Brasileira de Genética1999-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400010Genetics and Molecular Biology v.22 n.4 1999reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571999000400010info:eu-repo/semantics/openAccessRibeiro,Luciana A.Azevedo,João L.Aragão,Francisco J.L.Rech,Elibio L.Schmidt,Gilberto S.Coutinho,Luiz L.eng2000-02-17T00:00:00Zoai:scielo:S1415-47571999000400010Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2000-02-17T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
In situ DNA transfer to chicken embryos by biolistics |
title |
In situ DNA transfer to chicken embryos by biolistics |
spellingShingle |
In situ DNA transfer to chicken embryos by biolistics Ribeiro,Luciana A. |
title_short |
In situ DNA transfer to chicken embryos by biolistics |
title_full |
In situ DNA transfer to chicken embryos by biolistics |
title_fullStr |
In situ DNA transfer to chicken embryos by biolistics |
title_full_unstemmed |
In situ DNA transfer to chicken embryos by biolistics |
title_sort |
In situ DNA transfer to chicken embryos by biolistics |
author |
Ribeiro,Luciana A. |
author_facet |
Ribeiro,Luciana A. Azevedo,João L. Aragão,Francisco J.L. Rech,Elibio L. Schmidt,Gilberto S. Coutinho,Luiz L. |
author_role |
author |
author2 |
Azevedo,João L. Aragão,Francisco J.L. Rech,Elibio L. Schmidt,Gilberto S. Coutinho,Luiz L. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro,Luciana A. Azevedo,João L. Aragão,Francisco J.L. Rech,Elibio L. Schmidt,Gilberto S. Coutinho,Luiz L. |
description |
Fertilized chicken eggs were bombarded with a biolistic device. Transient expression of the lacZ gene under the control of a human cytomegalovirus (CMV) promoter was assessed after in situ gene transfer using this approach. The influence of different pressures, vacuum levels and particles was tested. Survival rate improved as particle velocity decreased, but resulted in lower levels of expression. The best survival and expression were obtained with gold particles, a helium gas pressure of 600 psi and a vacuum of 600 mmHg. Under these conditions, all bombarded embryos showed <FONT FACE="Symbol">b</FONT>-galactosidase activity, indicating that this was an effective method for transformation of chicken embryos. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47571999000400010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.22 n.4 1999 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122377417785344 |