Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale

Detalhes bibliográficos
Autor(a) principal: Nieddu,Mariella
Data de Publicação: 2015
Outros Autores: Mezzanotte,Roberto, Pichiri,Giuseppina, Coni,Pier Paolo, Dedola,Gian Luca, Dettori,Maria Luisa, Pazzola,Michele, Vacca,Giuseppe Massimo, Robledo,Renato
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513
Resumo: Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that did not show hybridization to probe Om1. No signal was detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1 showed almost 100% homology with a bacterial sequence reported in Helicobacter pylori.
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spelling Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary taleBovidaesatellite DNAfluorescence in situ hybridizationpericentromeric regionAbstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that did not show hybridization to probe Om1. No signal was detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1 showed almost 100% homology with a bacterial sequence reported in Helicobacter pylori.Sociedade Brasileira de Genética2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513Genetics and Molecular Biology v.38 n.4 2015reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-475738420150094info:eu-repo/semantics/openAccessNieddu,MariellaMezzanotte,RobertoPichiri,GiuseppinaConi,Pier PaoloDedola,Gian LucaDettori,Maria LuisaPazzola,MicheleVacca,Giuseppe MassimoRobledo,Renatoeng2016-02-02T00:00:00Zoai:scielo:S1415-47572015000400513Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2016-02-02T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
title Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
spellingShingle Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
Nieddu,Mariella
Bovidae
satellite DNA
fluorescence in situ hybridization
pericentromeric region
title_short Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
title_full Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
title_fullStr Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
title_full_unstemmed Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
title_sort Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
author Nieddu,Mariella
author_facet Nieddu,Mariella
Mezzanotte,Roberto
Pichiri,Giuseppina
Coni,Pier Paolo
Dedola,Gian Luca
Dettori,Maria Luisa
Pazzola,Michele
Vacca,Giuseppe Massimo
Robledo,Renato
author_role author
author2 Mezzanotte,Roberto
Pichiri,Giuseppina
Coni,Pier Paolo
Dedola,Gian Luca
Dettori,Maria Luisa
Pazzola,Michele
Vacca,Giuseppe Massimo
Robledo,Renato
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nieddu,Mariella
Mezzanotte,Roberto
Pichiri,Giuseppina
Coni,Pier Paolo
Dedola,Gian Luca
Dettori,Maria Luisa
Pazzola,Michele
Vacca,Giuseppe Massimo
Robledo,Renato
dc.subject.por.fl_str_mv Bovidae
satellite DNA
fluorescence in situ hybridization
pericentromeric region
topic Bovidae
satellite DNA
fluorescence in situ hybridization
pericentromeric region
description Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that did not show hybridization to probe Om1. No signal was detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1 showed almost 100% homology with a bacterial sequence reported in Helicobacter pylori.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-475738420150094
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.38 n.4 2015
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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