Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513 |
Resumo: | Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that did not show hybridization to probe Om1. No signal was detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1 showed almost 100% homology with a bacterial sequence reported in Helicobacter pylori. |
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Genetics and Molecular Biology |
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Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary taleBovidaesatellite DNAfluorescence in situ hybridizationpericentromeric regionAbstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that did not show hybridization to probe Om1. No signal was detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1 showed almost 100% homology with a bacterial sequence reported in Helicobacter pylori.Sociedade Brasileira de Genética2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513Genetics and Molecular Biology v.38 n.4 2015reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-475738420150094info:eu-repo/semantics/openAccessNieddu,MariellaMezzanotte,RobertoPichiri,GiuseppinaConi,Pier PaoloDedola,Gian LucaDettori,Maria LuisaPazzola,MicheleVacca,Giuseppe MassimoRobledo,Renatoeng2016-02-02T00:00:00Zoai:scielo:S1415-47572015000400513Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2016-02-02T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale |
title |
Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale |
spellingShingle |
Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale Nieddu,Mariella Bovidae satellite DNA fluorescence in situ hybridization pericentromeric region |
title_short |
Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale |
title_full |
Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale |
title_fullStr |
Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale |
title_full_unstemmed |
Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale |
title_sort |
Evolution of satellite DNA sequences in two tribes of Bovidae: A cautionary tale |
author |
Nieddu,Mariella |
author_facet |
Nieddu,Mariella Mezzanotte,Roberto Pichiri,Giuseppina Coni,Pier Paolo Dedola,Gian Luca Dettori,Maria Luisa Pazzola,Michele Vacca,Giuseppe Massimo Robledo,Renato |
author_role |
author |
author2 |
Mezzanotte,Roberto Pichiri,Giuseppina Coni,Pier Paolo Dedola,Gian Luca Dettori,Maria Luisa Pazzola,Michele Vacca,Giuseppe Massimo Robledo,Renato |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nieddu,Mariella Mezzanotte,Roberto Pichiri,Giuseppina Coni,Pier Paolo Dedola,Gian Luca Dettori,Maria Luisa Pazzola,Michele Vacca,Giuseppe Massimo Robledo,Renato |
dc.subject.por.fl_str_mv |
Bovidae satellite DNA fluorescence in situ hybridization pericentromeric region |
topic |
Bovidae satellite DNA fluorescence in situ hybridization pericentromeric region |
description |
Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that did not show hybridization to probe Om1. No signal was detected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1 showed almost 100% homology with a bacterial sequence reported in Helicobacter pylori. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-475738420150094 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.38 n.4 2015 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122386616942592 |