Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae

Detalhes bibliográficos
Autor(a) principal: Valadares-Inglis,Maria Cléria
Data de Publicação: 1998
Outros Autores: Peberdy,John F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100003
Resumo: Pulsed-field gel electrophoresis (PFGE) was used to separate chromosome-sized DNA molecules of four strains of Metarhizium anisopliae from Brazil. Metarhizium anisopliae isolates from Japan have been reported as possessing seven chromosomes. Variation was observed among the Brazilian strains and the chromosomal DNA was resolved into eight bands for strain CG46. Densitometric analysis of PFGE gels suggested that the other three Brazilian strains also possess eight chromosomes, with two chromosomes migrating as doublets under the electrophoretic conditions used. The genome size was estimated as varying between 23.39 to 31.88 Mb, not including possible doublet chromosomes.
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spelling Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliaePulsed-field gel electrophoresis (PFGE) was used to separate chromosome-sized DNA molecules of four strains of Metarhizium anisopliae from Brazil. Metarhizium anisopliae isolates from Japan have been reported as possessing seven chromosomes. Variation was observed among the Brazilian strains and the chromosomal DNA was resolved into eight bands for strain CG46. Densitometric analysis of PFGE gels suggested that the other three Brazilian strains also possess eight chromosomes, with two chromosomes migrating as doublets under the electrophoretic conditions used. The genome size was estimated as varying between 23.39 to 31.88 Mb, not including possible doublet chromosomes.Sociedade Brasileira de Genética1998-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100003Genetics and Molecular Biology v.21 n.1 1998reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571998000100003info:eu-repo/semantics/openAccessValadares-Inglis,Maria ClériaPeberdy,John F.eng1999-01-06T00:00:00Zoai:scielo:S1415-47571998000100003Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-01-06T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae
title Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae
spellingShingle Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae
Valadares-Inglis,Maria Cléria
title_short Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae
title_full Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae
title_fullStr Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae
title_full_unstemmed Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae
title_sort Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae
author Valadares-Inglis,Maria Cléria
author_facet Valadares-Inglis,Maria Cléria
Peberdy,John F.
author_role author
author2 Peberdy,John F.
author2_role author
dc.contributor.author.fl_str_mv Valadares-Inglis,Maria Cléria
Peberdy,John F.
description Pulsed-field gel electrophoresis (PFGE) was used to separate chromosome-sized DNA molecules of four strains of Metarhizium anisopliae from Brazil. Metarhizium anisopliae isolates from Japan have been reported as possessing seven chromosomes. Variation was observed among the Brazilian strains and the chromosomal DNA was resolved into eight bands for strain CG46. Densitometric analysis of PFGE gels suggested that the other three Brazilian strains also possess eight chromosomes, with two chromosomes migrating as doublets under the electrophoretic conditions used. The genome size was estimated as varying between 23.39 to 31.88 Mb, not including possible doublet chromosomes.
publishDate 1998
dc.date.none.fl_str_mv 1998-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47571998000100003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.21 n.1 1998
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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