Discrimination of Shark species by simple PCR of 5S rDNA repeats

Detalhes bibliográficos
Autor(a) principal: Pinhal,Danillo
Data de Publicação: 2008
Outros Autores: Gadig,Otto BF, Wasko,Adriane P, Oliveira,Claudio, Ron,Ernesto, Foresti,Fausto, Martins,Cesar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033
Resumo: Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to discriminate elasmobranch species. The described 5S rDNA PCR approach generated species-specific genetic markers that should find broad application in fishery management and trade of sharks and their subproducts.
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spelling Discrimination of Shark species by simple PCR of 5S rDNA repeatsChondrichthyesPCRspecies identification5S rDNAsharksSharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to discriminate elasmobranch species. The described 5S rDNA PCR approach generated species-specific genetic markers that should find broad application in fishery management and trade of sharks and their subproducts.Sociedade Brasileira de Genética2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033Genetics and Molecular Biology v.31 n.1 suppl.0 2008reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572008000200033info:eu-repo/semantics/openAccessPinhal,DanilloGadig,Otto BFWasko,Adriane POliveira,ClaudioRon,ErnestoForesti,FaustoMartins,Cesareng2008-06-03T00:00:00Zoai:scielo:S1415-47572008000200033Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2008-06-03T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Discrimination of Shark species by simple PCR of 5S rDNA repeats
title Discrimination of Shark species by simple PCR of 5S rDNA repeats
spellingShingle Discrimination of Shark species by simple PCR of 5S rDNA repeats
Pinhal,Danillo
Chondrichthyes
PCR
species identification
5S rDNA
sharks
title_short Discrimination of Shark species by simple PCR of 5S rDNA repeats
title_full Discrimination of Shark species by simple PCR of 5S rDNA repeats
title_fullStr Discrimination of Shark species by simple PCR of 5S rDNA repeats
title_full_unstemmed Discrimination of Shark species by simple PCR of 5S rDNA repeats
title_sort Discrimination of Shark species by simple PCR of 5S rDNA repeats
author Pinhal,Danillo
author_facet Pinhal,Danillo
Gadig,Otto BF
Wasko,Adriane P
Oliveira,Claudio
Ron,Ernesto
Foresti,Fausto
Martins,Cesar
author_role author
author2 Gadig,Otto BF
Wasko,Adriane P
Oliveira,Claudio
Ron,Ernesto
Foresti,Fausto
Martins,Cesar
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinhal,Danillo
Gadig,Otto BF
Wasko,Adriane P
Oliveira,Claudio
Ron,Ernesto
Foresti,Fausto
Martins,Cesar
dc.subject.por.fl_str_mv Chondrichthyes
PCR
species identification
5S rDNA
sharks
topic Chondrichthyes
PCR
species identification
5S rDNA
sharks
description Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to discriminate elasmobranch species. The described 5S rDNA PCR approach generated species-specific genetic markers that should find broad application in fishery management and trade of sharks and their subproducts.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572008000200033
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.31 n.1 suppl.0 2008
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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