Discrimination of Shark species by simple PCR of 5S rDNA repeats

Detalhes bibliográficos
Autor(a) principal: Pinhal, Danillo [UNESP]
Data de Publicação: 2008
Outros Autores: Gadig, Otto Bismarck Fazzano [UNESP], Wasko, Adriane Pinto [UNESP], Oliveira, Claudio [UNESP], Ron, Ernesto, Foresti, Fausto [UNESP], Martins, Cesar [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572008000200033
http://hdl.handle.net/11449/18474
Resumo: Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to discriminate elasmobranch species. The described 5S rDNA PCR approach generated species-specific genetic markers that should find broad application in fishery management and trade of sharks and their subproducts.
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spelling Discrimination of Shark species by simple PCR of 5S rDNA repeatsChondrichthyesPCRspecies identification5S rDNAsharksSharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to discriminate elasmobranch species. The described 5S rDNA PCR approach generated species-specific genetic markers that should find broad application in fishery management and trade of sharks and their subproducts.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaUniversidade Estadual PaulistaUniversidade Estadual Paulista Instituto de Biociências Departamento de GenéticaUniversidad de Oriente Escuela de Ciencias Aplicadas del MarUniversidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaUniversidade Estadual PaulistaUniversidade Estadual Paulista Instituto de Biociências Departamento de GenéticaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Londrina (UEL)Pinhal, Danillo [UNESP]Gadig, Otto Bismarck Fazzano [UNESP]Wasko, Adriane Pinto [UNESP]Oliveira, Claudio [UNESP]Ron, ErnestoForesti, Fausto [UNESP]Martins, Cesar [UNESP]2014-05-20T13:51:42Z2014-05-20T13:51:42Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article361-365application/pdfhttp://dx.doi.org/10.1590/S1415-47572008000200033Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 361-365, 2008.1415-4757http://hdl.handle.net/11449/1847410.1590/S1415-47572008000200033S1415-47572008000200033WOS:000257238500033S1415-47572008000200033.pdf2161551575581523192686391737890988588006994253520000-0003-4600-03670000-0001-8423-72990000-0003-3534-974XSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-12-28T06:18:34Zoai:repositorio.unesp.br:11449/18474Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:31:29.837640Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Discrimination of Shark species by simple PCR of 5S rDNA repeats
title Discrimination of Shark species by simple PCR of 5S rDNA repeats
spellingShingle Discrimination of Shark species by simple PCR of 5S rDNA repeats
Pinhal, Danillo [UNESP]
Chondrichthyes
PCR
species identification
5S rDNA
sharks
title_short Discrimination of Shark species by simple PCR of 5S rDNA repeats
title_full Discrimination of Shark species by simple PCR of 5S rDNA repeats
title_fullStr Discrimination of Shark species by simple PCR of 5S rDNA repeats
title_full_unstemmed Discrimination of Shark species by simple PCR of 5S rDNA repeats
title_sort Discrimination of Shark species by simple PCR of 5S rDNA repeats
author Pinhal, Danillo [UNESP]
author_facet Pinhal, Danillo [UNESP]
Gadig, Otto Bismarck Fazzano [UNESP]
Wasko, Adriane Pinto [UNESP]
Oliveira, Claudio [UNESP]
Ron, Ernesto
Foresti, Fausto [UNESP]
Martins, Cesar [UNESP]
author_role author
author2 Gadig, Otto Bismarck Fazzano [UNESP]
Wasko, Adriane Pinto [UNESP]
Oliveira, Claudio [UNESP]
Ron, Ernesto
Foresti, Fausto [UNESP]
Martins, Cesar [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Londrina (UEL)
dc.contributor.author.fl_str_mv Pinhal, Danillo [UNESP]
Gadig, Otto Bismarck Fazzano [UNESP]
Wasko, Adriane Pinto [UNESP]
Oliveira, Claudio [UNESP]
Ron, Ernesto
Foresti, Fausto [UNESP]
Martins, Cesar [UNESP]
dc.subject.por.fl_str_mv Chondrichthyes
PCR
species identification
5S rDNA
sharks
topic Chondrichthyes
PCR
species identification
5S rDNA
sharks
description Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to discriminate elasmobranch species. The described 5S rDNA PCR approach generated species-specific genetic markers that should find broad application in fishery management and trade of sharks and their subproducts.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2014-05-20T13:51:42Z
2014-05-20T13:51:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572008000200033
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 361-365, 2008.
1415-4757
http://hdl.handle.net/11449/18474
10.1590/S1415-47572008000200033
S1415-47572008000200033
WOS:000257238500033
S1415-47572008000200033.pdf
2161551575581523
1926863917378909
8858800699425352
0000-0003-4600-0367
0000-0001-8423-7299
0000-0003-3534-974X
url http://dx.doi.org/10.1590/S1415-47572008000200033
http://hdl.handle.net/11449/18474
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 361-365, 2008.
1415-4757
10.1590/S1415-47572008000200033
S1415-47572008000200033
WOS:000257238500033
S1415-47572008000200033.pdf
2161551575581523
1926863917378909
8858800699425352
0000-0003-4600-0367
0000-0001-8423-7299
0000-0003-3534-974X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 361-365
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129330243436544

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