Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations

Detalhes bibliográficos
Autor(a) principal: Tavares,Mara Garcia
Data de Publicação: 2013
Outros Autores: Pietrani,Nathalia Teixeira, Durvale,Maxwell de Castro, Resende,Helder Canto, Campos,Lucio Antonio de Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016
Resumo: Melipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23) indicated that the populations sampled were structured, and the clustering analysis showed the formation of two subgroups and two more distant populations. The first group contained the subspecies M. quadrifasciata quadrifasciata, and the other, the subspecies M. quadrifasciata anthidioides and the two M. quadrifasciata populations with continuous metasomal stripes from northern Minas Gerais. These results confirmed that the yellow metasomal stripes alone are not a good means for correctly identifying the different subspecies of M. quadrifasciata.
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spelling Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populationsgenetic differentiationmicrosatellitespopulation geneticsstingless beesMelipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23) indicated that the populations sampled were structured, and the clustering analysis showed the formation of two subgroups and two more distant populations. The first group contained the subspecies M. quadrifasciata quadrifasciata, and the other, the subspecies M. quadrifasciata anthidioides and the two M. quadrifasciata populations with continuous metasomal stripes from northern Minas Gerais. These results confirmed that the yellow metasomal stripes alone are not a good means for correctly identifying the different subspecies of M. quadrifasciata.Sociedade Brasileira de Genética2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016Genetics and Molecular Biology v.36 n.1 2013reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572013000100016info:eu-repo/semantics/openAccessTavares,Mara GarciaPietrani,Nathalia TeixeiraDurvale,Maxwell de CastroResende,Helder CantoCampos,Lucio Antonio de Oliveiraeng2013-02-28T00:00:00Zoai:scielo:S1415-47572013000100016Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2013-02-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
title Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
spellingShingle Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
Tavares,Mara Garcia
genetic differentiation
microsatellites
population genetics
stingless bees
title_short Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
title_full Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
title_fullStr Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
title_full_unstemmed Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
title_sort Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
author Tavares,Mara Garcia
author_facet Tavares,Mara Garcia
Pietrani,Nathalia Teixeira
Durvale,Maxwell de Castro
Resende,Helder Canto
Campos,Lucio Antonio de Oliveira
author_role author
author2 Pietrani,Nathalia Teixeira
Durvale,Maxwell de Castro
Resende,Helder Canto
Campos,Lucio Antonio de Oliveira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Tavares,Mara Garcia
Pietrani,Nathalia Teixeira
Durvale,Maxwell de Castro
Resende,Helder Canto
Campos,Lucio Antonio de Oliveira
dc.subject.por.fl_str_mv genetic differentiation
microsatellites
population genetics
stingless bees
topic genetic differentiation
microsatellites
population genetics
stingless bees
description Melipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23) indicated that the populations sampled were structured, and the clustering analysis showed the formation of two subgroups and two more distant populations. The first group contained the subspecies M. quadrifasciata quadrifasciata, and the other, the subspecies M. quadrifasciata anthidioides and the two M. quadrifasciata populations with continuous metasomal stripes from northern Minas Gerais. These results confirmed that the yellow metasomal stripes alone are not a good means for correctly identifying the different subspecies of M. quadrifasciata.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572013000100016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.36 n.1 2013
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
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reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
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