Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016 |
Resumo: | Melipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23) indicated that the populations sampled were structured, and the clustering analysis showed the formation of two subgroups and two more distant populations. The first group contained the subspecies M. quadrifasciata quadrifasciata, and the other, the subspecies M. quadrifasciata anthidioides and the two M. quadrifasciata populations with continuous metasomal stripes from northern Minas Gerais. These results confirmed that the yellow metasomal stripes alone are not a good means for correctly identifying the different subspecies of M. quadrifasciata. |
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Genetics and Molecular Biology |
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Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populationsgenetic differentiationmicrosatellitespopulation geneticsstingless beesMelipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23) indicated that the populations sampled were structured, and the clustering analysis showed the formation of two subgroups and two more distant populations. The first group contained the subspecies M. quadrifasciata quadrifasciata, and the other, the subspecies M. quadrifasciata anthidioides and the two M. quadrifasciata populations with continuous metasomal stripes from northern Minas Gerais. These results confirmed that the yellow metasomal stripes alone are not a good means for correctly identifying the different subspecies of M. quadrifasciata.Sociedade Brasileira de Genética2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016Genetics and Molecular Biology v.36 n.1 2013reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572013000100016info:eu-repo/semantics/openAccessTavares,Mara GarciaPietrani,Nathalia TeixeiraDurvale,Maxwell de CastroResende,Helder CantoCampos,Lucio Antonio de Oliveiraeng2013-02-28T00:00:00Zoai:scielo:S1415-47572013000100016Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2013-02-28T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations |
title |
Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations |
spellingShingle |
Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations Tavares,Mara Garcia genetic differentiation microsatellites population genetics stingless bees |
title_short |
Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations |
title_full |
Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations |
title_fullStr |
Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations |
title_full_unstemmed |
Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations |
title_sort |
Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations |
author |
Tavares,Mara Garcia |
author_facet |
Tavares,Mara Garcia Pietrani,Nathalia Teixeira Durvale,Maxwell de Castro Resende,Helder Canto Campos,Lucio Antonio de Oliveira |
author_role |
author |
author2 |
Pietrani,Nathalia Teixeira Durvale,Maxwell de Castro Resende,Helder Canto Campos,Lucio Antonio de Oliveira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Tavares,Mara Garcia Pietrani,Nathalia Teixeira Durvale,Maxwell de Castro Resende,Helder Canto Campos,Lucio Antonio de Oliveira |
dc.subject.por.fl_str_mv |
genetic differentiation microsatellites population genetics stingless bees |
topic |
genetic differentiation microsatellites population genetics stingless bees |
description |
Melipona quadrifasciata is a stingless bee widely found throughout the Brazilian territory, with two recognized subspecies, M. quadrifasciata anthidioides, that exhibits interrupted metasomal stripes, and M. quadrifasciata quadrifasciata, with continuous metasomal stripes. This study aimed to estimate the genetic variability of these subspecies. For this purpose, 127 colonies from 15 Brazilian localities were analyzed, using nine species-specific microsatellite primers. At these loci, the number of alleles ranged from three to 15 (mean: 7.2), and the observed heterozygosity (Ho) ranged from 0.03-0.21, while the expected heterozygosity (He) ranged from 0.23-0.47. The genetic distances among populations ranged from 0.03-0.45. The F ST multilocus value (0.23) indicated that the populations sampled were structured, and the clustering analysis showed the formation of two subgroups and two more distant populations. The first group contained the subspecies M. quadrifasciata quadrifasciata, and the other, the subspecies M. quadrifasciata anthidioides and the two M. quadrifasciata populations with continuous metasomal stripes from northern Minas Gerais. These results confirmed that the yellow metasomal stripes alone are not a good means for correctly identifying the different subspecies of M. quadrifasciata. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572013000100016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.36 n.1 2013 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122385389060096 |