Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1111/j.1755-0998.2008.02486.x http://www.locus.ufv.br/handle/123456789/15316 |
Resumo: | Eight microsatellite primers were developed from ISSR (intersimple sequence repeats) markers for the stingless bee Melipona rufiventris. These primers were tested in 20 M. rufiventris workers, representing a single population from Minas Gerais state. The number of alleles per locus ranged from 2 to 5 (mean = 2.63) and the observed and expected heterozygosity values ranged from 0.00 to 0.44 (mean = 0.20) and from 0.05 to 0.68 (mean = 0.31), respectively. Several loci were also polymorphic in M. quadrifasciata, M. bicolor, M. mandacaia and Partamona helleri and should prove useful in population studies of other stingless bees. |
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Lopes, Denilce MenesesSilva, Felipe Oliveira DaSalomão, Tânia Maria FernandesCampos, Lúcio Antônio De OliveiraTavares, Mara Gracia2017-12-18T10:11:29Z2017-12-18T10:11:29Z2008-10-151755-0998http://dx.doi.org/10.1111/j.1755-0998.2008.02486.xhttp://www.locus.ufv.br/handle/123456789/15316Eight microsatellite primers were developed from ISSR (intersimple sequence repeats) markers for the stingless bee Melipona rufiventris. These primers were tested in 20 M. rufiventris workers, representing a single population from Minas Gerais state. The number of alleles per locus ranged from 2 to 5 (mean = 2.63) and the observed and expected heterozygosity values ranged from 0.00 to 0.44 (mean = 0.20) and from 0.05 to 0.68 (mean = 0.31), respectively. Several loci were also polymorphic in M. quadrifasciata, M. bicolor, M. mandacaia and Partamona helleri and should prove useful in population studies of other stingless bees.engMolecular Ecology Resources9(3), p.923-925, May 2009ISSRMeliponaMicrosatellitePopulation geneticsStingless beesMicrosatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALLOPES_et_al-2009-Molecular_Ecology_Resources.pdfLOPES_et_al-2009-Molecular_Ecology_Resources.pdftexto completoapplication/pdf75119https://locus.ufv.br//bitstream/123456789/15316/1/LOPES_et_al-2009-Molecular_Ecology_Resources.pdff8f2d8b7b6782c6b420cce690d031aecMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/15316/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILLOPES_et_al-2009-Molecular_Ecology_Resources.pdf.jpgLOPES_et_al-2009-Molecular_Ecology_Resources.pdf.jpgIM Thumbnailimage/jpeg5121https://locus.ufv.br//bitstream/123456789/15316/3/LOPES_et_al-2009-Molecular_Ecology_Resources.pdf.jpga220aaeae5593aa8a75502ce79aa1ee8MD53123456789/153162017-12-18 22:02:00.39oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-12-19T01:02LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.en.fl_str_mv |
Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae) |
title |
Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae) |
spellingShingle |
Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae) Lopes, Denilce Meneses ISSR Melipona Microsatellite Population genetics Stingless bees |
title_short |
Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae) |
title_full |
Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae) |
title_fullStr |
Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae) |
title_full_unstemmed |
Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae) |
title_sort |
Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae) |
author |
Lopes, Denilce Meneses |
author_facet |
Lopes, Denilce Meneses Silva, Felipe Oliveira Da Salomão, Tânia Maria Fernandes Campos, Lúcio Antônio De Oliveira Tavares, Mara Gracia |
author_role |
author |
author2 |
Silva, Felipe Oliveira Da Salomão, Tânia Maria Fernandes Campos, Lúcio Antônio De Oliveira Tavares, Mara Gracia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lopes, Denilce Meneses Silva, Felipe Oliveira Da Salomão, Tânia Maria Fernandes Campos, Lúcio Antônio De Oliveira Tavares, Mara Gracia |
dc.subject.pt-BR.fl_str_mv |
ISSR Melipona Microsatellite Population genetics Stingless bees |
topic |
ISSR Melipona Microsatellite Population genetics Stingless bees |
description |
Eight microsatellite primers were developed from ISSR (intersimple sequence repeats) markers for the stingless bee Melipona rufiventris. These primers were tested in 20 M. rufiventris workers, representing a single population from Minas Gerais state. The number of alleles per locus ranged from 2 to 5 (mean = 2.63) and the observed and expected heterozygosity values ranged from 0.00 to 0.44 (mean = 0.20) and from 0.05 to 0.68 (mean = 0.31), respectively. Several loci were also polymorphic in M. quadrifasciata, M. bicolor, M. mandacaia and Partamona helleri and should prove useful in population studies of other stingless bees. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-10-15 |
dc.date.accessioned.fl_str_mv |
2017-12-18T10:11:29Z |
dc.date.available.fl_str_mv |
2017-12-18T10:11:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1755-0998.2008.02486.x http://www.locus.ufv.br/handle/123456789/15316 |
dc.identifier.issn.none.fl_str_mv |
1755-0998 |
identifier_str_mv |
1755-0998 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2008.02486.x http://www.locus.ufv.br/handle/123456789/15316 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofseries.pt-BR.fl_str_mv |
9(3), p.923-925, May 2009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Molecular Ecology Resources |
publisher.none.fl_str_mv |
Molecular Ecology Resources |
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Universidade Federal de Viçosa (UFV) |
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UFV |
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LOCUS Repositório Institucional da UFV |
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