Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466 |
Resumo: | Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic variability in this parasite. We developed an assay that employs a reporter gene that, once mutated in specific positions, convert G418-sensitive into G418-insenstitive T. cruzi. We were able to determine the frequency of DNA mutations in T. cruzi exposed and non-exposed to oxidative insults assessing the number of colony-forming units in solid selective media after plating a defined number of cells. We verified that T. cruzi‘s spontaneous mutation frequency was comparable to those found in other eukaryotes, and that exposure to hydrogen peroxide promoted a two-fold increase in T. cruzi‘s mutation frequency. We hypothesize that genetic mutations in T. cruzi can arise from oxidative insults faced by this parasite during its life cycle. |
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Genetics and Molecular Biology |
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Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruziT. cruziDNAmutation frequencyH2O2Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic variability in this parasite. We developed an assay that employs a reporter gene that, once mutated in specific positions, convert G418-sensitive into G418-insenstitive T. cruzi. We were able to determine the frequency of DNA mutations in T. cruzi exposed and non-exposed to oxidative insults assessing the number of colony-forming units in solid selective media after plating a defined number of cells. We verified that T. cruzi‘s spontaneous mutation frequency was comparable to those found in other eukaryotes, and that exposure to hydrogen peroxide promoted a two-fold increase in T. cruzi‘s mutation frequency. We hypothesize that genetic mutations in T. cruzi can arise from oxidative insults faced by this parasite during its life cycle.Sociedade Brasileira de Genética2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466Genetics and Molecular Biology v.41 n.2 2018reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2017-0281info:eu-repo/semantics/openAccessTorres-Silva,Carolina FurtadoRepolês,Bruno MarçalOrnelas,Hugo OliveiraMacedo,Andréa MaraFranco,Glória ReginaJunho Pena,Sérgio DaniloTahara,Erich BirelliMachado,Carlos Renatoeng2018-06-21T00:00:00Zoai:scielo:S1415-47572018000300466Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2018-06-21T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi |
title |
Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi |
spellingShingle |
Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi Torres-Silva,Carolina Furtado T. cruzi DNA mutation frequency H2O2 |
title_short |
Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi |
title_full |
Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi |
title_fullStr |
Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi |
title_full_unstemmed |
Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi |
title_sort |
Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi |
author |
Torres-Silva,Carolina Furtado |
author_facet |
Torres-Silva,Carolina Furtado Repolês,Bruno Marçal Ornelas,Hugo Oliveira Macedo,Andréa Mara Franco,Glória Regina Junho Pena,Sérgio Danilo Tahara,Erich Birelli Machado,Carlos Renato |
author_role |
author |
author2 |
Repolês,Bruno Marçal Ornelas,Hugo Oliveira Macedo,Andréa Mara Franco,Glória Regina Junho Pena,Sérgio Danilo Tahara,Erich Birelli Machado,Carlos Renato |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Torres-Silva,Carolina Furtado Repolês,Bruno Marçal Ornelas,Hugo Oliveira Macedo,Andréa Mara Franco,Glória Regina Junho Pena,Sérgio Danilo Tahara,Erich Birelli Machado,Carlos Renato |
dc.subject.por.fl_str_mv |
T. cruzi DNA mutation frequency H2O2 |
topic |
T. cruzi DNA mutation frequency H2O2 |
description |
Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic variability in this parasite. We developed an assay that employs a reporter gene that, once mutated in specific positions, convert G418-sensitive into G418-insenstitive T. cruzi. We were able to determine the frequency of DNA mutations in T. cruzi exposed and non-exposed to oxidative insults assessing the number of colony-forming units in solid selective media after plating a defined number of cells. We verified that T. cruzi‘s spontaneous mutation frequency was comparable to those found in other eukaryotes, and that exposure to hydrogen peroxide promoted a two-fold increase in T. cruzi‘s mutation frequency. We hypothesize that genetic mutations in T. cruzi can arise from oxidative insults faced by this parasite during its life cycle. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2017-0281 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.41 n.2 2018 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122388823146496 |