Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Torres-Silva,Carolina Furtado
Data de Publicação: 2018
Outros Autores: Repolês,Bruno Marçal, Ornelas,Hugo Oliveira, Macedo,Andréa Mara, Franco,Glória Regina, Junho Pena,Sérgio Danilo, Tahara,Erich Birelli, Machado,Carlos Renato
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466
Resumo: Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic variability in this parasite. We developed an assay that employs a reporter gene that, once mutated in specific positions, convert G418-sensitive into G418-insenstitive T. cruzi. We were able to determine the frequency of DNA mutations in T. cruzi exposed and non-exposed to oxidative insults assessing the number of colony-forming units in solid selective media after plating a defined number of cells. We verified that T. cruzi‘s spontaneous mutation frequency was comparable to those found in other eukaryotes, and that exposure to hydrogen peroxide promoted a two-fold increase in T. cruzi‘s mutation frequency. We hypothesize that genetic mutations in T. cruzi can arise from oxidative insults faced by this parasite during its life cycle.
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spelling Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruziT. cruziDNAmutation frequencyH2O2Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic variability in this parasite. We developed an assay that employs a reporter gene that, once mutated in specific positions, convert G418-sensitive into G418-insenstitive T. cruzi. We were able to determine the frequency of DNA mutations in T. cruzi exposed and non-exposed to oxidative insults assessing the number of colony-forming units in solid selective media after plating a defined number of cells. We verified that T. cruzi‘s spontaneous mutation frequency was comparable to those found in other eukaryotes, and that exposure to hydrogen peroxide promoted a two-fold increase in T. cruzi‘s mutation frequency. We hypothesize that genetic mutations in T. cruzi can arise from oxidative insults faced by this parasite during its life cycle.Sociedade Brasileira de Genética2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466Genetics and Molecular Biology v.41 n.2 2018reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2017-0281info:eu-repo/semantics/openAccessTorres-Silva,Carolina FurtadoRepolês,Bruno MarçalOrnelas,Hugo OliveiraMacedo,Andréa MaraFranco,Glória ReginaJunho Pena,Sérgio DaniloTahara,Erich BirelliMachado,Carlos Renatoeng2018-06-21T00:00:00Zoai:scielo:S1415-47572018000300466Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2018-06-21T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
title Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
spellingShingle Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
Torres-Silva,Carolina Furtado
T. cruzi
DNA
mutation frequency
H2O2
title_short Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
title_full Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
title_fullStr Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
title_full_unstemmed Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
title_sort Assessment of genetic mutation frequency induced by oxidative stress in Trypanosoma cruzi
author Torres-Silva,Carolina Furtado
author_facet Torres-Silva,Carolina Furtado
Repolês,Bruno Marçal
Ornelas,Hugo Oliveira
Macedo,Andréa Mara
Franco,Glória Regina
Junho Pena,Sérgio Danilo
Tahara,Erich Birelli
Machado,Carlos Renato
author_role author
author2 Repolês,Bruno Marçal
Ornelas,Hugo Oliveira
Macedo,Andréa Mara
Franco,Glória Regina
Junho Pena,Sérgio Danilo
Tahara,Erich Birelli
Machado,Carlos Renato
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Torres-Silva,Carolina Furtado
Repolês,Bruno Marçal
Ornelas,Hugo Oliveira
Macedo,Andréa Mara
Franco,Glória Regina
Junho Pena,Sérgio Danilo
Tahara,Erich Birelli
Machado,Carlos Renato
dc.subject.por.fl_str_mv T. cruzi
DNA
mutation frequency
H2O2
topic T. cruzi
DNA
mutation frequency
H2O2
description Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic variability in this parasite. We developed an assay that employs a reporter gene that, once mutated in specific positions, convert G418-sensitive into G418-insenstitive T. cruzi. We were able to determine the frequency of DNA mutations in T. cruzi exposed and non-exposed to oxidative insults assessing the number of colony-forming units in solid selective media after plating a defined number of cells. We verified that T. cruzi‘s spontaneous mutation frequency was comparable to those found in other eukaryotes, and that exposure to hydrogen peroxide promoted a two-fold increase in T. cruzi‘s mutation frequency. We hypothesize that genetic mutations in T. cruzi can arise from oxidative insults faced by this parasite during its life cycle.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2017-0281
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.41 n.2 2018
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
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reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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