Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472

Detalhes bibliográficos
Autor(a) principal: Baraúna,Rafael A.
Data de Publicação: 2015
Outros Autores: Santos,Agenor V., Graças,Diego A., Santos,Daniel M., Ghilardi Júnior,Rubens, Pimenta,Adriano M. C., Carepo,Marta S. P., Schneider,Maria P.C., Silva,Artur
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200227
Resumo: Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.
id SBG-1_77d28a9e248e52f1bdeac2b841b57e43
oai_identifier_str oai:scielo:S1415-47572015000200227
network_acronym_str SBG-1
network_name_str Genetics and Molecular Biology
repository_id_str
spelling Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472C. violaceumelectromagnetic fieldproteomic analysisSeveral studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.Sociedade Brasileira de Genética2015-06-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200227Genetics and Molecular Biology v.38 n.2 2015reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-4757382220140240info:eu-repo/semantics/openAccessBaraúna,Rafael A.Santos,Agenor V.Graças,Diego A.Santos,Daniel M.Ghilardi Júnior,RubensPimenta,Adriano M. C.Carepo,Marta S. P.Schneider,Maria P.C.Silva,Artureng2015-07-31T00:00:00Zoai:scielo:S1415-47572015000200227Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2015-07-31T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
title Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
spellingShingle Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
Baraúna,Rafael A.
C. violaceum
electromagnetic field
proteomic analysis
title_short Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
title_full Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
title_fullStr Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
title_full_unstemmed Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
title_sort Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472
author Baraúna,Rafael A.
author_facet Baraúna,Rafael A.
Santos,Agenor V.
Graças,Diego A.
Santos,Daniel M.
Ghilardi Júnior,Rubens
Pimenta,Adriano M. C.
Carepo,Marta S. P.
Schneider,Maria P.C.
Silva,Artur
author_role author
author2 Santos,Agenor V.
Graças,Diego A.
Santos,Daniel M.
Ghilardi Júnior,Rubens
Pimenta,Adriano M. C.
Carepo,Marta S. P.
Schneider,Maria P.C.
Silva,Artur
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Baraúna,Rafael A.
Santos,Agenor V.
Graças,Diego A.
Santos,Daniel M.
Ghilardi Júnior,Rubens
Pimenta,Adriano M. C.
Carepo,Marta S. P.
Schneider,Maria P.C.
Silva,Artur
dc.subject.por.fl_str_mv C. violaceum
electromagnetic field
proteomic analysis
topic C. violaceum
electromagnetic field
proteomic analysis
description Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200227
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200227
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-4757382220140240
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.38 n.2 2015
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
_version_ 1752122386303418368