Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceum ATCC 12472

• Autor(a) principal: BARAÚNA, Rafael Azevedo
• Data de Publicação: 2015
• Outros Autores: SANTOS, Agenor Valadares, GRAÇAS, Diego Assis das, SANTOS, Daniel Moreira dos, GHILARDI JUNIOR, Rubens, PIMENTA, Adriano Monteiro de Castro, CAREPO, Marta Sofia Peixe, SCHNEIDER, Maria Paula Cruz, SILVA, Artur Luiz da Costa da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFPA
• Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/6865
• topic: Campos eletromagnéticos; Análise proteômica; Chromobacterium violaceum

Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.