Microbial quality of soil from the Pampa biome in response to different grazing pressures
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200205 |
Resumo: | The aim of this study was to evaluate the impact of different grazing pressures on the activity and diversity of soil bacteria. We performed a long-term experiment in Eldorado do Sul, southern Brazil, that assessed three levels of grazing pressure: high pressure (HP), with 4% herbage allowance (HA), moderate pressure (MP), with 12% HA, and low pressure (LP), with 16% HA. Two reference areas were also assessed, one of never-grazed native vegetation (NG) and another of regenerated vegetation after two years of grazing (RG). Soil samples were evaluated for microbial biomass and enzymatic (β-glucosidase, arylsulfatase and urease) activities. The structure of the bacterial community and the population of diazotrophic bacteria were evaluated by RFLP of the 16S rRNA and nifH genes, respectively. The diversity of diazotrophic bacteria was assessed by partial sequencing of the 16S rDNA gene. The presence of grazing animals increased soil microbial biomass in MP and HP. The structures of the bacterial community and the populations of diazotrophic bacteria were altered by the different grazing managements, with a greater diversity of diazotrophic bacteria in the LP treatment. Based on the characteristics evaluated, the MP treatment was the most appropriate for animal production and conservation of the Pampa biome. |
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Genetics and Molecular Biology |
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Microbial quality of soil from the Pampa biome in response to different grazing pressuresbacterial diversitydiazotrophic bacteriadiversitygrasslandssoil microbial communitiesThe aim of this study was to evaluate the impact of different grazing pressures on the activity and diversity of soil bacteria. We performed a long-term experiment in Eldorado do Sul, southern Brazil, that assessed three levels of grazing pressure: high pressure (HP), with 4% herbage allowance (HA), moderate pressure (MP), with 12% HA, and low pressure (LP), with 16% HA. Two reference areas were also assessed, one of never-grazed native vegetation (NG) and another of regenerated vegetation after two years of grazing (RG). Soil samples were evaluated for microbial biomass and enzymatic (β-glucosidase, arylsulfatase and urease) activities. The structure of the bacterial community and the population of diazotrophic bacteria were evaluated by RFLP of the 16S rRNA and nifH genes, respectively. The diversity of diazotrophic bacteria was assessed by partial sequencing of the 16S rDNA gene. The presence of grazing animals increased soil microbial biomass in MP and HP. The structures of the bacterial community and the populations of diazotrophic bacteria were altered by the different grazing managements, with a greater diversity of diazotrophic bacteria in the LP treatment. Based on the characteristics evaluated, the MP treatment was the most appropriate for animal production and conservation of the Pampa biome.Sociedade Brasileira de Genética2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200205Genetics and Molecular Biology v.38 n.2 2015reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-475738138120140230info:eu-repo/semantics/openAccessVargas,Rafael S.Bataiolli,Renatada Costa,Pedro B.Lisboa,BrunoPassaglia,Luciane Maria P.Beneduzi,AneliseVargas,Luciano K.eng2015-07-31T00:00:00Zoai:scielo:S1415-47572015000200205Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2015-07-31T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Microbial quality of soil from the Pampa biome in response to different grazing pressures |
title |
Microbial quality of soil from the Pampa biome in response to different grazing pressures |
spellingShingle |
Microbial quality of soil from the Pampa biome in response to different grazing pressures Vargas,Rafael S. bacterial diversity diazotrophic bacteria diversity grasslands soil microbial communities |
title_short |
Microbial quality of soil from the Pampa biome in response to different grazing pressures |
title_full |
Microbial quality of soil from the Pampa biome in response to different grazing pressures |
title_fullStr |
Microbial quality of soil from the Pampa biome in response to different grazing pressures |
title_full_unstemmed |
Microbial quality of soil from the Pampa biome in response to different grazing pressures |
title_sort |
Microbial quality of soil from the Pampa biome in response to different grazing pressures |
author |
Vargas,Rafael S. |
author_facet |
Vargas,Rafael S. Bataiolli,Renata da Costa,Pedro B. Lisboa,Bruno Passaglia,Luciane Maria P. Beneduzi,Anelise Vargas,Luciano K. |
author_role |
author |
author2 |
Bataiolli,Renata da Costa,Pedro B. Lisboa,Bruno Passaglia,Luciane Maria P. Beneduzi,Anelise Vargas,Luciano K. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Vargas,Rafael S. Bataiolli,Renata da Costa,Pedro B. Lisboa,Bruno Passaglia,Luciane Maria P. Beneduzi,Anelise Vargas,Luciano K. |
dc.subject.por.fl_str_mv |
bacterial diversity diazotrophic bacteria diversity grasslands soil microbial communities |
topic |
bacterial diversity diazotrophic bacteria diversity grasslands soil microbial communities |
description |
The aim of this study was to evaluate the impact of different grazing pressures on the activity and diversity of soil bacteria. We performed a long-term experiment in Eldorado do Sul, southern Brazil, that assessed three levels of grazing pressure: high pressure (HP), with 4% herbage allowance (HA), moderate pressure (MP), with 12% HA, and low pressure (LP), with 16% HA. Two reference areas were also assessed, one of never-grazed native vegetation (NG) and another of regenerated vegetation after two years of grazing (RG). Soil samples were evaluated for microbial biomass and enzymatic (β-glucosidase, arylsulfatase and urease) activities. The structure of the bacterial community and the population of diazotrophic bacteria were evaluated by RFLP of the 16S rRNA and nifH genes, respectively. The diversity of diazotrophic bacteria was assessed by partial sequencing of the 16S rDNA gene. The presence of grazing animals increased soil microbial biomass in MP and HP. The structures of the bacterial community and the populations of diazotrophic bacteria were altered by the different grazing managements, with a greater diversity of diazotrophic bacteria in the LP treatment. Based on the characteristics evaluated, the MP treatment was the most appropriate for animal production and conservation of the Pampa biome. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200205 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200205 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-475738138120140230 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.38 n.2 2015 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122386298175488 |