Sequence diversity and copy number variation of Mutator-like transposases in wheat

Detalhes bibliográficos
Autor(a) principal: Asakura,Nobuaki
Data de Publicação: 2008
Outros Autores: Yoshida,Shinya, Mori,Naoki, Ohtsuka,Ichiro, Nakamura,Chiharu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300022
Resumo: Partial transposase-coding sequences of Mutator-like elements (MULEs) were isolated from a wild einkorn wheat, Triticum urartu, by degenerate PCR. The isolated sequences were classified into a MuDR or Class I clade and divided into two distinct subclasses (subclass I and subclass II). The average pair-wise identity between members of both subclasses was 58.8% at the nucleotide sequence level. Sequence diversity of subclass I was larger than that of subclass II. DNA gel blot analysis showed that subclass I was present as low copy number elements in the genomes of all Triticum and Aegilops accessions surveyed, while subclass II was present as high copy number elements. These two subclasses seemed uncapable of recognizing each other for transposition. The number of copies of subclass II elements was much higher in Aegilops with the S, Sl and D genomes and polyploid Triticum species than in diploid Triticum with the A genome, indicating that active transposition occurred in S, Sl and D genomes before polyploidization. DNA gel blot analysis of six species selected from three subfamilies of Poaceae demonstrated that only the tribe Triticeae possessed both subclasses. These results suggest that the differentiation of these two subclasses occurred before or immediately after the establishment of the tribe Triticeae.
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spelling Sequence diversity and copy number variation of Mutator-like transposases in wheatMutator-like transposasesequence diversitycopy number variationTriticumAegilopsPartial transposase-coding sequences of Mutator-like elements (MULEs) were isolated from a wild einkorn wheat, Triticum urartu, by degenerate PCR. The isolated sequences were classified into a MuDR or Class I clade and divided into two distinct subclasses (subclass I and subclass II). The average pair-wise identity between members of both subclasses was 58.8% at the nucleotide sequence level. Sequence diversity of subclass I was larger than that of subclass II. DNA gel blot analysis showed that subclass I was present as low copy number elements in the genomes of all Triticum and Aegilops accessions surveyed, while subclass II was present as high copy number elements. These two subclasses seemed uncapable of recognizing each other for transposition. The number of copies of subclass II elements was much higher in Aegilops with the S, Sl and D genomes and polyploid Triticum species than in diploid Triticum with the A genome, indicating that active transposition occurred in S, Sl and D genomes before polyploidization. DNA gel blot analysis of six species selected from three subfamilies of Poaceae demonstrated that only the tribe Triticeae possessed both subclasses. These results suggest that the differentiation of these two subclasses occurred before or immediately after the establishment of the tribe Triticeae.Sociedade Brasileira de Genética2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300022Genetics and Molecular Biology v.31 n.2 2008reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572008000300022info:eu-repo/semantics/openAccessAsakura,NobuakiYoshida,ShinyaMori,NaokiOhtsuka,IchiroNakamura,Chiharueng2008-06-24T00:00:00Zoai:scielo:S1415-47572008000300022Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2008-06-24T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Sequence diversity and copy number variation of Mutator-like transposases in wheat
title Sequence diversity and copy number variation of Mutator-like transposases in wheat
spellingShingle Sequence diversity and copy number variation of Mutator-like transposases in wheat
Asakura,Nobuaki
Mutator-like transposase
sequence diversity
copy number variation
Triticum
Aegilops
title_short Sequence diversity and copy number variation of Mutator-like transposases in wheat
title_full Sequence diversity and copy number variation of Mutator-like transposases in wheat
title_fullStr Sequence diversity and copy number variation of Mutator-like transposases in wheat
title_full_unstemmed Sequence diversity and copy number variation of Mutator-like transposases in wheat
title_sort Sequence diversity and copy number variation of Mutator-like transposases in wheat
author Asakura,Nobuaki
author_facet Asakura,Nobuaki
Yoshida,Shinya
Mori,Naoki
Ohtsuka,Ichiro
Nakamura,Chiharu
author_role author
author2 Yoshida,Shinya
Mori,Naoki
Ohtsuka,Ichiro
Nakamura,Chiharu
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Asakura,Nobuaki
Yoshida,Shinya
Mori,Naoki
Ohtsuka,Ichiro
Nakamura,Chiharu
dc.subject.por.fl_str_mv Mutator-like transposase
sequence diversity
copy number variation
Triticum
Aegilops
topic Mutator-like transposase
sequence diversity
copy number variation
Triticum
Aegilops
description Partial transposase-coding sequences of Mutator-like elements (MULEs) were isolated from a wild einkorn wheat, Triticum urartu, by degenerate PCR. The isolated sequences were classified into a MuDR or Class I clade and divided into two distinct subclasses (subclass I and subclass II). The average pair-wise identity between members of both subclasses was 58.8% at the nucleotide sequence level. Sequence diversity of subclass I was larger than that of subclass II. DNA gel blot analysis showed that subclass I was present as low copy number elements in the genomes of all Triticum and Aegilops accessions surveyed, while subclass II was present as high copy number elements. These two subclasses seemed uncapable of recognizing each other for transposition. The number of copies of subclass II elements was much higher in Aegilops with the S, Sl and D genomes and polyploid Triticum species than in diploid Triticum with the A genome, indicating that active transposition occurred in S, Sl and D genomes before polyploidization. DNA gel blot analysis of six species selected from three subfamilies of Poaceae demonstrated that only the tribe Triticeae possessed both subclasses. These results suggest that the differentiation of these two subclasses occurred before or immediately after the establishment of the tribe Triticeae.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572008000300022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.31 n.2 2008
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
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reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
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