Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth

Detalhes bibliográficos
Autor(a) principal: Souza, Fernanda Timm Seabra
Data de Publicação: 2004
Outros Autores: Mello, Alexandre Silva de, Michelin, Kristiane, Coelho, Janice Carneiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/23375
Resumo: The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and glucocerebrosidase was affected in the presence of amikacin. Vancomycin did not interfere with any of the parameters analyzed, so it was chosen to be used in cell cultures to prevent the contamination by gram positive bacteria.
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spelling Souza, Fernanda Timm SeabraMello, Alexandre Silva deMichelin, KristianeCoelho, Janice Carneiro2010-06-05T04:17:22Z20041415-4757http://hdl.handle.net/10183/23375000472202The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and glucocerebrosidase was affected in the presence of amikacin. Vancomycin did not interfere with any of the parameters analyzed, so it was chosen to be used in cell cultures to prevent the contamination by gram positive bacteria.application/pdfengGenetics and molecular biology. Ribeirão Preto. Vol. 27, no. 3 (Sept. 2004), p. 454-459AmicacinaCefalotinaClindamicinaVancomicinaFibroblastosAmikacinCephalothinClindamycinVancomycinFibroblastsCell cultureEffect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growthinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000472202.pdf000472202.pdfTexto completo (inglês)application/pdf417483http://www.lume.ufrgs.br/bitstream/10183/23375/1/000472202.pdf7fa081026a44ffa99b6d56bf46397e05MD51TEXT000472202.pdf.txt000472202.pdf.txtExtracted Texttext/plain18476http://www.lume.ufrgs.br/bitstream/10183/23375/2/000472202.pdf.txt7f1e64e88769e611461033de7a86230bMD52THUMBNAIL000472202.pdf.jpg000472202.pdf.jpgGenerated Thumbnailimage/jpeg1696http://www.lume.ufrgs.br/bitstream/10183/23375/3/000472202.pdf.jpg91472e4b1574a9273876757e30478f35MD5310183/233752023-09-16 03:41:21.564193oai:www.lume.ufrgs.br:10183/23375Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-16T06:41:21Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
spellingShingle Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
Souza, Fernanda Timm Seabra
Amicacina
Cefalotina
Clindamicina
Vancomicina
Fibroblastos
Amikacin
Cephalothin
Clindamycin
Vancomycin
Fibroblasts
Cell culture
title_short Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title_full Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title_fullStr Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title_full_unstemmed Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
title_sort Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth
author Souza, Fernanda Timm Seabra
author_facet Souza, Fernanda Timm Seabra
Mello, Alexandre Silva de
Michelin, Kristiane
Coelho, Janice Carneiro
author_role author
author2 Mello, Alexandre Silva de
Michelin, Kristiane
Coelho, Janice Carneiro
author2_role author
author
author
dc.contributor.author.fl_str_mv Souza, Fernanda Timm Seabra
Mello, Alexandre Silva de
Michelin, Kristiane
Coelho, Janice Carneiro
dc.subject.por.fl_str_mv Amicacina
Cefalotina
Clindamicina
Vancomicina
Fibroblastos
topic Amicacina
Cefalotina
Clindamicina
Vancomicina
Fibroblastos
Amikacin
Cephalothin
Clindamycin
Vancomycin
Fibroblasts
Cell culture
dc.subject.eng.fl_str_mv Amikacin
Cephalothin
Clindamycin
Vancomycin
Fibroblasts
Cell culture
description The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and glucocerebrosidase was affected in the presence of amikacin. Vancomycin did not interfere with any of the parameters analyzed, so it was chosen to be used in cell cultures to prevent the contamination by gram positive bacteria.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2010-06-05T04:17:22Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/23375
dc.identifier.issn.pt_BR.fl_str_mv 1415-4757
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Genetics and molecular biology. Ribeirão Preto. Vol. 27, no. 3 (Sept. 2004), p. 454-459
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