Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200014 |
Resumo: | Experiments with novobiocin (NB) post-treatment were performed to verify its effect on the frequencies of micronuclei (MN) and chromosomal aberrations (CA) induced by <FONT FACE="Symbol">g</font>-irradiation (0.75, 1.5 and 3.0 Gy) in human lymphocytes at G0-phase. The frequencies of MN significantly decreased by 44 and 50%, for the treatment with NB 50 µg/ml (30-min pulse) after radiation doses of 1.5 and 3.0 Gy, respectively. However, CA frequencies were not significantly affected. No significant effect on CA was observed when lymphocyte cultures were exposed to a single dose of 2.0 Gy at the G0-phase and posttreated with 25 µg/ml NB for three hours either immediately after irradiation (G0-phase) or after 24 h (S-phase). The significant suppressive effect of NB on MN frequencies supports the hypothesis that NB interaction with chromatin increases access to DNA repair enzymes. |
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Genetics and Molecular Biology |
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Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpointsExperiments with novobiocin (NB) post-treatment were performed to verify its effect on the frequencies of micronuclei (MN) and chromosomal aberrations (CA) induced by <FONT FACE="Symbol">g</font>-irradiation (0.75, 1.5 and 3.0 Gy) in human lymphocytes at G0-phase. The frequencies of MN significantly decreased by 44 and 50%, for the treatment with NB 50 µg/ml (30-min pulse) after radiation doses of 1.5 and 3.0 Gy, respectively. However, CA frequencies were not significantly affected. No significant effect on CA was observed when lymphocyte cultures were exposed to a single dose of 2.0 Gy at the G0-phase and posttreated with 25 µg/ml NB for three hours either immediately after irradiation (G0-phase) or after 24 h (S-phase). The significant suppressive effect of NB on MN frequencies supports the hypothesis that NB interaction with chromatin increases access to DNA repair enzymes.Sociedade Brasileira de Genética1999-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200014Genetics and Molecular Biology v.22 n.2 1999reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47571999000200014info:eu-repo/semantics/openAccessSavoldi-Barbosa,MarcelaSakamoto-Hojo,Elza T.Takahashi,Catarina S.eng1999-08-26T00:00:00Zoai:scielo:S1415-47571999000200014Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:1999-08-26T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints |
title |
Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints |
spellingShingle |
Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints Savoldi-Barbosa,Marcela |
title_short |
Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints |
title_full |
Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints |
title_fullStr |
Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints |
title_full_unstemmed |
Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints |
title_sort |
Influence of novobiocin on g-irradiation G0-lymphocytes as analyzed by cytogenetic endpoints |
author |
Savoldi-Barbosa,Marcela |
author_facet |
Savoldi-Barbosa,Marcela Sakamoto-Hojo,Elza T. Takahashi,Catarina S. |
author_role |
author |
author2 |
Sakamoto-Hojo,Elza T. Takahashi,Catarina S. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Savoldi-Barbosa,Marcela Sakamoto-Hojo,Elza T. Takahashi,Catarina S. |
description |
Experiments with novobiocin (NB) post-treatment were performed to verify its effect on the frequencies of micronuclei (MN) and chromosomal aberrations (CA) induced by <FONT FACE="Symbol">g</font>-irradiation (0.75, 1.5 and 3.0 Gy) in human lymphocytes at G0-phase. The frequencies of MN significantly decreased by 44 and 50%, for the treatment with NB 50 µg/ml (30-min pulse) after radiation doses of 1.5 and 3.0 Gy, respectively. However, CA frequencies were not significantly affected. No significant effect on CA was observed when lymphocyte cultures were exposed to a single dose of 2.0 Gy at the G0-phase and posttreated with 25 µg/ml NB for three hours either immediately after irradiation (G0-phase) or after 24 h (S-phase). The significant suppressive effect of NB on MN frequencies supports the hypothesis that NB interaction with chromatin increases access to DNA repair enzymes. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47571999000200014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.22 n.2 1999 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122377353822208 |