Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranae
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000300202 |
Resumo: | Abstract Supernumerary, or B, chromosomes are present in several eukaryotes, including characid fish of the genus Psalidodon. Notably, Psalidodon paranae carries the most studied B chromosome variant, a macro-B chromosome. The origin of this element was determined to be an isochromosome; however, data regarding its inheritance remain unavailable due to methodological barriers such as the lack of an efficient, non-invasive, and rapid protocol for identifying B-carrying individuals that would enable the design of efficient crossing experiments. Thus, in this study, we primarily aimed was to develop two non-invasive and fast (approximately 2 h) methods to identify the presence of B chromosomes in live specimens of P. paranae based on satellite DNA (satDNA) sequences known to be present in this element. The methods include fluorescence in situ hybridization in interphase nuclei and relative gene quantification of satDNAs using quantitative polymerase chain reaction. Our results reveal the efficiency of quick-fluorescence in situ hybridization and quantitative polymerase chain reaction for identifying B-carrying individuals using the proposed satDNA sequences and open up new possibilities to study B chromosomes. |
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Genetics and Molecular Biology |
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Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranaePsalidodonNeotropical fishessatellite DNAqPCRB chromosomesAbstract Supernumerary, or B, chromosomes are present in several eukaryotes, including characid fish of the genus Psalidodon. Notably, Psalidodon paranae carries the most studied B chromosome variant, a macro-B chromosome. The origin of this element was determined to be an isochromosome; however, data regarding its inheritance remain unavailable due to methodological barriers such as the lack of an efficient, non-invasive, and rapid protocol for identifying B-carrying individuals that would enable the design of efficient crossing experiments. Thus, in this study, we primarily aimed was to develop two non-invasive and fast (approximately 2 h) methods to identify the presence of B chromosomes in live specimens of P. paranae based on satellite DNA (satDNA) sequences known to be present in this element. The methods include fluorescence in situ hybridization in interphase nuclei and relative gene quantification of satDNAs using quantitative polymerase chain reaction. Our results reveal the efficiency of quick-fluorescence in situ hybridization and quantitative polymerase chain reaction for identifying B-carrying individuals using the proposed satDNA sequences and open up new possibilities to study B chromosomes.Sociedade Brasileira de Genética2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000300202Genetics and Molecular Biology v.44 n.2 2021reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2020-0003info:eu-repo/semantics/openAccessGoes,Caio Augusto GomesSilva,Duílio Mazzoni Zerbinato de AndradeUtsunomia,RicardoYasui,George ShiguekiArtoni,Roberto FerreiraForesti,FaustoPorto-Foresti,Fábioeng2021-03-24T00:00:00Zoai:scielo:S1415-47572021000300202Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2021-03-24T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranae |
title |
Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranae |
spellingShingle |
Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranae Goes,Caio Augusto Gomes Psalidodon Neotropical fishes satellite DNA qPCR B chromosomes |
title_short |
Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranae |
title_full |
Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranae |
title_fullStr |
Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranae |
title_full_unstemmed |
Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranae |
title_sort |
Establishment of rapid and non-invasive protocols to identify B-carrying individuals of Psalidodon paranae |
author |
Goes,Caio Augusto Gomes |
author_facet |
Goes,Caio Augusto Gomes Silva,Duílio Mazzoni Zerbinato de Andrade Utsunomia,Ricardo Yasui,George Shigueki Artoni,Roberto Ferreira Foresti,Fausto Porto-Foresti,Fábio |
author_role |
author |
author2 |
Silva,Duílio Mazzoni Zerbinato de Andrade Utsunomia,Ricardo Yasui,George Shigueki Artoni,Roberto Ferreira Foresti,Fausto Porto-Foresti,Fábio |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Goes,Caio Augusto Gomes Silva,Duílio Mazzoni Zerbinato de Andrade Utsunomia,Ricardo Yasui,George Shigueki Artoni,Roberto Ferreira Foresti,Fausto Porto-Foresti,Fábio |
dc.subject.por.fl_str_mv |
Psalidodon Neotropical fishes satellite DNA qPCR B chromosomes |
topic |
Psalidodon Neotropical fishes satellite DNA qPCR B chromosomes |
description |
Abstract Supernumerary, or B, chromosomes are present in several eukaryotes, including characid fish of the genus Psalidodon. Notably, Psalidodon paranae carries the most studied B chromosome variant, a macro-B chromosome. The origin of this element was determined to be an isochromosome; however, data regarding its inheritance remain unavailable due to methodological barriers such as the lack of an efficient, non-invasive, and rapid protocol for identifying B-carrying individuals that would enable the design of efficient crossing experiments. Thus, in this study, we primarily aimed was to develop two non-invasive and fast (approximately 2 h) methods to identify the presence of B chromosomes in live specimens of P. paranae based on satellite DNA (satDNA) sequences known to be present in this element. The methods include fluorescence in situ hybridization in interphase nuclei and relative gene quantification of satDNAs using quantitative polymerase chain reaction. Our results reveal the efficiency of quick-fluorescence in situ hybridization and quantitative polymerase chain reaction for identifying B-carrying individuals using the proposed satDNA sequences and open up new possibilities to study B chromosomes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000300202 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000300202 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4685-gmb-2020-0003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.44 n.2 2021 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122390494576640 |