Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster

Detalhes bibliográficos
Autor(a) principal: Costa,Wender Ferreira
Data de Publicação: 2010
Outros Autores: Oliveira,Alaide Braga de, Nepomuceno,Júlio César
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300029
Resumo: This study investigated the genotoxicity of Lapachol (LAP) evaluated by wing spot test of Drosophila melanogaster in the descendants from standard (ST) and high bioactivation (HB) crosses. This assay detects the loss of heterozygosity of marker genes expressed phenotypically on the fly's wings. Drosophila has extensive genetic homology to mammals, which makes it a suitable model organism for genotoxic investigations. Three-day-old larvae from ST crosses (females flr³/TM3, Bd s x males mwh/mwh), with basal levels of the cytochrome P450 and larvae of high metabolic bioactivity capacity (HB cross) (females ORR; flr³/TM3, Bd s x males mwh/mwh), were used. The results showed that LAP is a promutagen, exhibiting genotoxic activity in larvae from the HB cross. In other words, an increase in the frequency of spots is exclusive of individuals with a high level of the cytochrome P450. The results also indicate that recombinogenicity is the main genotoxic event induced by LAP.
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spelling Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogasterDrosophila melanogasterSMARTlapacholdoxorubicincytochrome P450This study investigated the genotoxicity of Lapachol (LAP) evaluated by wing spot test of Drosophila melanogaster in the descendants from standard (ST) and high bioactivation (HB) crosses. This assay detects the loss of heterozygosity of marker genes expressed phenotypically on the fly's wings. Drosophila has extensive genetic homology to mammals, which makes it a suitable model organism for genotoxic investigations. Three-day-old larvae from ST crosses (females flr³/TM3, Bd s x males mwh/mwh), with basal levels of the cytochrome P450 and larvae of high metabolic bioactivity capacity (HB cross) (females ORR; flr³/TM3, Bd s x males mwh/mwh), were used. The results showed that LAP is a promutagen, exhibiting genotoxic activity in larvae from the HB cross. In other words, an increase in the frequency of spots is exclusive of individuals with a high level of the cytochrome P450. The results also indicate that recombinogenicity is the main genotoxic event induced by LAP.Sociedade Brasileira de Genética2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300029Genetics and Molecular Biology v.33 n.3 2010reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572010005000070info:eu-repo/semantics/openAccessCosta,Wender FerreiraOliveira,Alaide Braga deNepomuceno,Júlio Césareng2010-08-18T00:00:00Zoai:scielo:S1415-47572010000300029Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2010-08-18T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster
title Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster
spellingShingle Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster
Costa,Wender Ferreira
Drosophila melanogaster
SMART
lapachol
doxorubicin
cytochrome P450
title_short Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster
title_full Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster
title_fullStr Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster
title_full_unstemmed Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster
title_sort Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster
author Costa,Wender Ferreira
author_facet Costa,Wender Ferreira
Oliveira,Alaide Braga de
Nepomuceno,Júlio César
author_role author
author2 Oliveira,Alaide Braga de
Nepomuceno,Júlio César
author2_role author
author
dc.contributor.author.fl_str_mv Costa,Wender Ferreira
Oliveira,Alaide Braga de
Nepomuceno,Júlio César
dc.subject.por.fl_str_mv Drosophila melanogaster
SMART
lapachol
doxorubicin
cytochrome P450
topic Drosophila melanogaster
SMART
lapachol
doxorubicin
cytochrome P450
description This study investigated the genotoxicity of Lapachol (LAP) evaluated by wing spot test of Drosophila melanogaster in the descendants from standard (ST) and high bioactivation (HB) crosses. This assay detects the loss of heterozygosity of marker genes expressed phenotypically on the fly's wings. Drosophila has extensive genetic homology to mammals, which makes it a suitable model organism for genotoxic investigations. Three-day-old larvae from ST crosses (females flr³/TM3, Bd s x males mwh/mwh), with basal levels of the cytochrome P450 and larvae of high metabolic bioactivity capacity (HB cross) (females ORR; flr³/TM3, Bd s x males mwh/mwh), were used. The results showed that LAP is a promutagen, exhibiting genotoxic activity in larvae from the HB cross. In other words, an increase in the frequency of spots is exclusive of individuals with a high level of the cytochrome P450. The results also indicate that recombinogenicity is the main genotoxic event induced by LAP.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300029
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572010005000070
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.33 n.3 2010
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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