Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements

Detalhes bibliográficos
Autor(a) principal: Liang,Qiaoyi
Data de Publicação: 2009
Outros Autores: Ding,Jiayi, Zheng,Shu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005
Resumo: Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs) identified 50 other LTR homogenous HERV-H elements. Further analysis of these elements revealed that all are structurally incomplete and only five exert transcriptional activity. The results presented here recommend further investigation into a potentially functional HERV-H-related ncRNA.
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spelling Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elementsendogenous retrovirusnoncoding RNART-PCRquantitative real-time PCRtranscriptionUp-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs) identified 50 other LTR homogenous HERV-H elements. Further analysis of these elements revealed that all are structurally incomplete and only five exert transcriptional activity. The results presented here recommend further investigation into a potentially functional HERV-H-related ncRNA.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005Genetics and Molecular Biology v.32 n.4 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000082info:eu-repo/semantics/openAccessLiang,QiaoyiDing,JiayiZheng,Shueng2009-11-17T00:00:00Zoai:scielo:S1415-47572009000400005Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-11-17T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
title Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
spellingShingle Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
Liang,Qiaoyi
endogenous retrovirus
noncoding RNA
RT-PCR
quantitative real-time PCR
transcription
title_short Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
title_full Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
title_fullStr Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
title_full_unstemmed Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
title_sort Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
author Liang,Qiaoyi
author_facet Liang,Qiaoyi
Ding,Jiayi
Zheng,Shu
author_role author
author2 Ding,Jiayi
Zheng,Shu
author2_role author
author
dc.contributor.author.fl_str_mv Liang,Qiaoyi
Ding,Jiayi
Zheng,Shu
dc.subject.por.fl_str_mv endogenous retrovirus
noncoding RNA
RT-PCR
quantitative real-time PCR
transcription
topic endogenous retrovirus
noncoding RNA
RT-PCR
quantitative real-time PCR
transcription
description Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs) identified 50 other LTR homogenous HERV-H elements. Further analysis of these elements revealed that all are structurally incomplete and only five exert transcriptional activity. The results presented here recommend further investigation into a potentially functional HERV-H-related ncRNA.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572009005000082
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.32 n.4 2009
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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