Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005 |
Resumo: | Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs) identified 50 other LTR homogenous HERV-H elements. Further analysis of these elements revealed that all are structurally incomplete and only five exert transcriptional activity. The results presented here recommend further investigation into a potentially functional HERV-H-related ncRNA. |
id |
SBG-1_bde4c08eefb0999470388891177db2ab |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572009000400005 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elementsendogenous retrovirusnoncoding RNART-PCRquantitative real-time PCRtranscriptionUp-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs) identified 50 other LTR homogenous HERV-H elements. Further analysis of these elements revealed that all are structurally incomplete and only five exert transcriptional activity. The results presented here recommend further investigation into a potentially functional HERV-H-related ncRNA.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005Genetics and Molecular Biology v.32 n.4 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000082info:eu-repo/semantics/openAccessLiang,QiaoyiDing,JiayiZheng,Shueng2009-11-17T00:00:00Zoai:scielo:S1415-47572009000400005Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-11-17T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements |
title |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements |
spellingShingle |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements Liang,Qiaoyi endogenous retrovirus noncoding RNA RT-PCR quantitative real-time PCR transcription |
title_short |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements |
title_full |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements |
title_fullStr |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements |
title_full_unstemmed |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements |
title_sort |
Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements |
author |
Liang,Qiaoyi |
author_facet |
Liang,Qiaoyi Ding,Jiayi Zheng,Shu |
author_role |
author |
author2 |
Ding,Jiayi Zheng,Shu |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Liang,Qiaoyi Ding,Jiayi Zheng,Shu |
dc.subject.por.fl_str_mv |
endogenous retrovirus noncoding RNA RT-PCR quantitative real-time PCR transcription |
topic |
endogenous retrovirus noncoding RNA RT-PCR quantitative real-time PCR transcription |
description |
Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs) identified 50 other LTR homogenous HERV-H elements. Further analysis of these elements revealed that all are structurally incomplete and only five exert transcriptional activity. The results presented here recommend further investigation into a potentially functional HERV-H-related ncRNA. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572009005000082 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.32 n.4 2009 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122382371258368 |