Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia

Detalhes bibliográficos
Autor(a) principal: Otero,Luize
Data de Publicação: 2005
Outros Autores: Cavalcanti Júnior,Geraldo Barroso, Klumb,Claudete Esteves, Scheiner,Marcos Antonio Mauricio, Magluta,Eliane Pereira Simões, Fernandez,Teresa de Souza, Silva,Maria Luiza Macedo, Pires,Virgínia, Andrade,Gabriela Vasconcelos, Maia,Raquel Ciuvalschi, Tabak,Daniel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100007
Resumo: chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.
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spelling Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemiachronic myeloid leukemiachromosome 17TP53 genep53 proteinchronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.Sociedade Brasileira de Genética2005-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100007Genetics and Molecular Biology v.28 n.1 2005reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572005000100007info:eu-repo/semantics/openAccessOtero,LuizeCavalcanti Júnior,Geraldo BarrosoKlumb,Claudete EstevesScheiner,Marcos Antonio MauricioMagluta,Eliane Pereira SimõesFernandez,Teresa de SouzaSilva,Maria Luiza MacedoPires,VirgíniaAndrade,Gabriela VasconcelosMaia,Raquel CiuvalschiTabak,Danieleng2005-09-08T00:00:00Zoai:scielo:S1415-47572005000100007Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2005-09-08T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
spellingShingle Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
Otero,Luize
chronic myeloid leukemia
chromosome 17
TP53 gene
p53 protein
title_short Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title_full Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title_fullStr Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title_full_unstemmed Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
title_sort Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
author Otero,Luize
author_facet Otero,Luize
Cavalcanti Júnior,Geraldo Barroso
Klumb,Claudete Esteves
Scheiner,Marcos Antonio Mauricio
Magluta,Eliane Pereira Simões
Fernandez,Teresa de Souza
Silva,Maria Luiza Macedo
Pires,Virgínia
Andrade,Gabriela Vasconcelos
Maia,Raquel Ciuvalschi
Tabak,Daniel
author_role author
author2 Cavalcanti Júnior,Geraldo Barroso
Klumb,Claudete Esteves
Scheiner,Marcos Antonio Mauricio
Magluta,Eliane Pereira Simões
Fernandez,Teresa de Souza
Silva,Maria Luiza Macedo
Pires,Virgínia
Andrade,Gabriela Vasconcelos
Maia,Raquel Ciuvalschi
Tabak,Daniel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Otero,Luize
Cavalcanti Júnior,Geraldo Barroso
Klumb,Claudete Esteves
Scheiner,Marcos Antonio Mauricio
Magluta,Eliane Pereira Simões
Fernandez,Teresa de Souza
Silva,Maria Luiza Macedo
Pires,Virgínia
Andrade,Gabriela Vasconcelos
Maia,Raquel Ciuvalschi
Tabak,Daniel
dc.subject.por.fl_str_mv chronic myeloid leukemia
chromosome 17
TP53 gene
p53 protein
topic chronic myeloid leukemia
chromosome 17
TP53 gene
p53 protein
description chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.
publishDate 2005
dc.date.none.fl_str_mv 2005-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572005000100007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.28 n.1 2005
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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