In silicio expression analysis of PKS genes isolated from Cannabis sativa L.

Detalhes bibliográficos
Autor(a) principal: Flores-Sanchez,Isvett J.
Data de Publicação: 2010
Outros Autores: Linthorst,Huub J.M., Verpoorte,Robert
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400017
Resumo: Cannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs) are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids and stilbenoids. Biosynthetically, the cannabinoids are polyketide substituted with terpenoid moiety. Using an RT-PCR homology search, PKS cDNAs were isolated from cannabis plants. The deduced amino acid sequences showed 51%-73% identity to other CHS/STS type sequences of the PKS family. Further, phylogenetic analysis revealed that these PKS cDNAs grouped with other non-chalcone-producing PKSs. Homology modeling analysis of these cannabis PKSs predicts a 3D overall fold, similar to alfalfa CHS2, with small steric differences on the residues that shape the active site of the cannabis PKSs.
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spelling In silicio expression analysis of PKS genes isolated from Cannabis sativa L.Cannabis sativahomology modelingpolyketide synthasesRT-PCRCannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs) are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids and stilbenoids. Biosynthetically, the cannabinoids are polyketide substituted with terpenoid moiety. Using an RT-PCR homology search, PKS cDNAs were isolated from cannabis plants. The deduced amino acid sequences showed 51%-73% identity to other CHS/STS type sequences of the PKS family. Further, phylogenetic analysis revealed that these PKS cDNAs grouped with other non-chalcone-producing PKSs. Homology modeling analysis of these cannabis PKSs predicts a 3D overall fold, similar to alfalfa CHS2, with small steric differences on the residues that shape the active site of the cannabis PKSs.Sociedade Brasileira de Genética2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400017Genetics and Molecular Biology v.33 n.4 2010reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572010005000088info:eu-repo/semantics/openAccessFlores-Sanchez,Isvett J.Linthorst,Huub J.M.Verpoorte,Roberteng2011-01-06T00:00:00Zoai:scielo:S1415-47572010000400017Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2011-01-06T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
title In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
spellingShingle In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
Flores-Sanchez,Isvett J.
Cannabis sativa
homology modeling
polyketide synthases
RT-PCR
title_short In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
title_full In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
title_fullStr In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
title_full_unstemmed In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
title_sort In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
author Flores-Sanchez,Isvett J.
author_facet Flores-Sanchez,Isvett J.
Linthorst,Huub J.M.
Verpoorte,Robert
author_role author
author2 Linthorst,Huub J.M.
Verpoorte,Robert
author2_role author
author
dc.contributor.author.fl_str_mv Flores-Sanchez,Isvett J.
Linthorst,Huub J.M.
Verpoorte,Robert
dc.subject.por.fl_str_mv Cannabis sativa
homology modeling
polyketide synthases
RT-PCR
topic Cannabis sativa
homology modeling
polyketide synthases
RT-PCR
description Cannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs) are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids and stilbenoids. Biosynthetically, the cannabinoids are polyketide substituted with terpenoid moiety. Using an RT-PCR homology search, PKS cDNAs were isolated from cannabis plants. The deduced amino acid sequences showed 51%-73% identity to other CHS/STS type sequences of the PKS family. Further, phylogenetic analysis revealed that these PKS cDNAs grouped with other non-chalcone-producing PKSs. Homology modeling analysis of these cannabis PKSs predicts a 3D overall fold, similar to alfalfa CHS2, with small steric differences on the residues that shape the active site of the cannabis PKSs.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400017
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572010005000088
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.33 n.4 2010
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
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