Detection and characterization of Wolbachia infection in silkworm

Detalhes bibliográficos
Autor(a) principal: Zha,Xingfu
Data de Publicação: 2014
Outros Autores: Zhang,Wenji, Zhou,Chunyan, Zhang,Liying, Xiang,Zhonghuai, Xia,Qingyou
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400014
Resumo: Wolbachia naturally infects a wide variety of arthropods, where it plays important roles in host reproduction. It was previously reported that Wolbachia did not infect silkworm. By means of PCR and sequencing we found in this study that Wolbachia is indeed present in silkworm. Phylogenetic analysis indicates that Wolbachia infection in silkworm may have occurred via transfer from parasitic wasps. Furthermore, Southern blotting results suggest a lateral transfer of the wsp gene into the genomes of some wild silkworms. By antibiotic treatments, we found that tetracycline and ciprofloxacin can eliminate Wolbachia in the silkworm and Wolbachia is important to ovary development of silkworm. These results provide clues towards a more comprehensive understanding of the interaction between Wolbachia and silkworm and possibly other lepidopteran insects.
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spelling Detection and characterization of Wolbachia infection in silkwormWolbachiasilkwormwspantibioticsWolbachia naturally infects a wide variety of arthropods, where it plays important roles in host reproduction. It was previously reported that Wolbachia did not infect silkworm. By means of PCR and sequencing we found in this study that Wolbachia is indeed present in silkworm. Phylogenetic analysis indicates that Wolbachia infection in silkworm may have occurred via transfer from parasitic wasps. Furthermore, Southern blotting results suggest a lateral transfer of the wsp gene into the genomes of some wild silkworms. By antibiotic treatments, we found that tetracycline and ciprofloxacin can eliminate Wolbachia in the silkworm and Wolbachia is important to ovary development of silkworm. These results provide clues towards a more comprehensive understanding of the interaction between Wolbachia and silkworm and possibly other lepidopteran insects.Sociedade Brasileira de Genética2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400014Genetics and Molecular Biology v.37 n.3 2014reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572014000400014info:eu-repo/semantics/openAccessZha,XingfuZhang,WenjiZhou,ChunyanZhang,LiyingXiang,ZhonghuaiXia,Qingyoueng2014-09-22T00:00:00Zoai:scielo:S1415-47572014000400014Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2014-09-22T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Detection and characterization of Wolbachia infection in silkworm
title Detection and characterization of Wolbachia infection in silkworm
spellingShingle Detection and characterization of Wolbachia infection in silkworm
Zha,Xingfu
Wolbachia
silkworm
wsp
antibiotics
title_short Detection and characterization of Wolbachia infection in silkworm
title_full Detection and characterization of Wolbachia infection in silkworm
title_fullStr Detection and characterization of Wolbachia infection in silkworm
title_full_unstemmed Detection and characterization of Wolbachia infection in silkworm
title_sort Detection and characterization of Wolbachia infection in silkworm
author Zha,Xingfu
author_facet Zha,Xingfu
Zhang,Wenji
Zhou,Chunyan
Zhang,Liying
Xiang,Zhonghuai
Xia,Qingyou
author_role author
author2 Zhang,Wenji
Zhou,Chunyan
Zhang,Liying
Xiang,Zhonghuai
Xia,Qingyou
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Zha,Xingfu
Zhang,Wenji
Zhou,Chunyan
Zhang,Liying
Xiang,Zhonghuai
Xia,Qingyou
dc.subject.por.fl_str_mv Wolbachia
silkworm
wsp
antibiotics
topic Wolbachia
silkworm
wsp
antibiotics
description Wolbachia naturally infects a wide variety of arthropods, where it plays important roles in host reproduction. It was previously reported that Wolbachia did not infect silkworm. By means of PCR and sequencing we found in this study that Wolbachia is indeed present in silkworm. Phylogenetic analysis indicates that Wolbachia infection in silkworm may have occurred via transfer from parasitic wasps. Furthermore, Southern blotting results suggest a lateral transfer of the wsp gene into the genomes of some wild silkworms. By antibiotic treatments, we found that tetracycline and ciprofloxacin can eliminate Wolbachia in the silkworm and Wolbachia is important to ovary development of silkworm. These results provide clues towards a more comprehensive understanding of the interaction between Wolbachia and silkworm and possibly other lepidopteran insects.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572014000400014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.37 n.3 2014
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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