Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)

Detalhes bibliográficos
Autor(a) principal: Deng,Ming-hua
Data de Publicação: 2020
Outros Autores: Zhao,Kai, Lv,Jun-heng, Huo,Jin-long, Zhang,Zhu-qing, Zhu,Hai-shan, Zou,Xue-xiao, Wen,Jin-fen
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400303
Resumo: Abstract The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different developmental stages of pepper flower [sporogenous cell division, stage (B1); pollen mother cell meiosis, stage (B2); and open flower (B3)]. In the cDNA libraries for B1, B2, and B3: 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34,445 unigene sequences and 128 pathways were annotated by KEGG pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified, and 8,955, 12,182, and 23,667 DEGs were identified in the B2 vs B1, B3 vs B1, and B3 vs. B2 comparisons. DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. According to the RNA-seq data, all 13 selected DEGs showed similar expression patterns after q-PCR analysis. Sucrose-phosphatase, galactinol-sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The new results provide valuable genetic information about flower development in pepper.
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spelling Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)Capsicum annuum L.flower transcriptomenectar biosynthesisnectary developmentgeneAbstract The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different developmental stages of pepper flower [sporogenous cell division, stage (B1); pollen mother cell meiosis, stage (B2); and open flower (B3)]. In the cDNA libraries for B1, B2, and B3: 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34,445 unigene sequences and 128 pathways were annotated by KEGG pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified, and 8,955, 12,182, and 23,667 DEGs were identified in the B2 vs B1, B3 vs B1, and B3 vs. B2 comparisons. DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. According to the RNA-seq data, all 13 selected DEGs showed similar expression patterns after q-PCR analysis. Sucrose-phosphatase, galactinol-sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The new results provide valuable genetic information about flower development in pepper.Sociedade Brasileira de Genética2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400303Genetics and Molecular Biology v.43 n.2 2020reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2018-0267info:eu-repo/semantics/openAccessDeng,Ming-huaZhao,KaiLv,Jun-hengHuo,Jin-longZhang,Zhu-qingZhu,Hai-shanZou,Xue-xiaoWen,Jin-feneng2020-05-26T00:00:00Zoai:scielo:S1415-47572020000400303Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2020-05-26T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)
title Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)
spellingShingle Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)
Deng,Ming-hua
Capsicum annuum L.
flower transcriptome
nectar biosynthesis
nectary development
gene
title_short Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)
title_full Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)
title_fullStr Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)
title_full_unstemmed Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)
title_sort Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)
author Deng,Ming-hua
author_facet Deng,Ming-hua
Zhao,Kai
Lv,Jun-heng
Huo,Jin-long
Zhang,Zhu-qing
Zhu,Hai-shan
Zou,Xue-xiao
Wen,Jin-fen
author_role author
author2 Zhao,Kai
Lv,Jun-heng
Huo,Jin-long
Zhang,Zhu-qing
Zhu,Hai-shan
Zou,Xue-xiao
Wen,Jin-fen
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Deng,Ming-hua
Zhao,Kai
Lv,Jun-heng
Huo,Jin-long
Zhang,Zhu-qing
Zhu,Hai-shan
Zou,Xue-xiao
Wen,Jin-fen
dc.subject.por.fl_str_mv Capsicum annuum L.
flower transcriptome
nectar biosynthesis
nectary development
gene
topic Capsicum annuum L.
flower transcriptome
nectar biosynthesis
nectary development
gene
description Abstract The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different developmental stages of pepper flower [sporogenous cell division, stage (B1); pollen mother cell meiosis, stage (B2); and open flower (B3)]. In the cDNA libraries for B1, B2, and B3: 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34,445 unigene sequences and 128 pathways were annotated by KEGG pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified, and 8,955, 12,182, and 23,667 DEGs were identified in the B2 vs B1, B3 vs B1, and B3 vs. B2 comparisons. DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. According to the RNA-seq data, all 13 selected DEGs showed similar expression patterns after q-PCR analysis. Sucrose-phosphatase, galactinol-sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The new results provide valuable genetic information about flower development in pepper.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2018-0267
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.43 n.2 2020
reponame:Genetics and Molecular Biology
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