Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy

Detalhes bibliográficos
Autor(a) principal: Lima,A.S.G.
Data de Publicação: 2002
Outros Autores: Guidelli,A.M., Abreu,I.L., Lemos,M.V.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200017
Resumo: PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.
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spelling Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopyREPERICcrystal proteinparasporal bodiesPCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.Sociedade Brasileira de Genética2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200017Genetics and Molecular Biology v.25 n.2 2002reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572002000200017info:eu-repo/semantics/openAccessLima,A.S.G.Guidelli,A.M.Abreu,I.L.Lemos,M.V.F.eng2002-09-11T00:00:00Zoai:scielo:S1415-47572002000200017Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2002-09-11T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
spellingShingle Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
Lima,A.S.G.
REP
ERIC
crystal protein
parasporal bodies
title_short Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title_full Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title_fullStr Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title_full_unstemmed Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title_sort Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
author Lima,A.S.G.
author_facet Lima,A.S.G.
Guidelli,A.M.
Abreu,I.L.
Lemos,M.V.F.
author_role author
author2 Guidelli,A.M.
Abreu,I.L.
Lemos,M.V.F.
author2_role author
author
author
dc.contributor.author.fl_str_mv Lima,A.S.G.
Guidelli,A.M.
Abreu,I.L.
Lemos,M.V.F.
dc.subject.por.fl_str_mv REP
ERIC
crystal protein
parasporal bodies
topic REP
ERIC
crystal protein
parasporal bodies
description PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572002000200017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.25 n.2 2002
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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