Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy

Detalhes bibliográficos
Autor(a) principal: Lima, Ana Silvia Gouvêa
Data de Publicação: 2002
Outros Autores: Guidelli, Ana Maria [UNESP], Abreu, Irlan Leite de [UNESP], Lemos, Manuel Victor Franco [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572002000200017
http://hdl.handle.net/11449/1029
Resumo: PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.
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spelling Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopyREPERICcrystal proteinparasporal bodiesPCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)USP Departamento de GenéticaUNESP Departamento de Biologia Aplicada à AgropecuáriaUNESP Departamento de Biologia Aplicada à AgropecuáriaSociedade Brasileira de GenéticaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Lima, Ana Silvia GouvêaGuidelli, Ana Maria [UNESP]Abreu, Irlan Leite de [UNESP]Lemos, Manuel Victor Franco [UNESP]2014-05-20T13:13:09Z2014-05-20T13:13:09Z2002-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article225-229application/pdfhttp://dx.doi.org/10.1590/S1415-47572002000200017Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 2, p. 225-229, 2002.1415-4757http://hdl.handle.net/11449/102910.1590/S1415-47572002000200017S1415-475720020002000172-s2.0-0036620718S1415-47572002000200017.pdf7179273060624761SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-10-04T06:04:44Zoai:repositorio.unesp.br:11449/1029Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T11:27:35.029380Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
spellingShingle Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
Lima, Ana Silvia Gouvêa
REP
ERIC
crystal protein
parasporal bodies
title_short Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title_full Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title_fullStr Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title_full_unstemmed Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
title_sort Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
author Lima, Ana Silvia Gouvêa
author_facet Lima, Ana Silvia Gouvêa
Guidelli, Ana Maria [UNESP]
Abreu, Irlan Leite de [UNESP]
Lemos, Manuel Victor Franco [UNESP]
author_role author
author2 Guidelli, Ana Maria [UNESP]
Abreu, Irlan Leite de [UNESP]
Lemos, Manuel Victor Franco [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Lima, Ana Silvia Gouvêa
Guidelli, Ana Maria [UNESP]
Abreu, Irlan Leite de [UNESP]
Lemos, Manuel Victor Franco [UNESP]
dc.subject.por.fl_str_mv REP
ERIC
crystal protein
parasporal bodies
topic REP
ERIC
crystal protein
parasporal bodies
description PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
2014-05-20T13:13:09Z
2014-05-20T13:13:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572002000200017
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 2, p. 225-229, 2002.
1415-4757
http://hdl.handle.net/11449/1029
10.1590/S1415-47572002000200017
S1415-47572002000200017
2-s2.0-0036620718
S1415-47572002000200017.pdf
7179273060624761
url http://dx.doi.org/10.1590/S1415-47572002000200017
http://hdl.handle.net/11449/1029
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 25, n. 2, p. 225-229, 2002.
1415-4757
10.1590/S1415-47572002000200017
S1415-47572002000200017
2-s2.0-0036620718
S1415-47572002000200017.pdf
7179273060624761
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 225-229
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803045826645196800

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