Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Neotropical ichthyology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211 |
Resumo: | Trichomycterus is a specious fish genus within Trichomycterinae and displays remarkable karyotype diversity. However, knowledge about their genomic structure and location of repetitive sequence is still limited. In order to better understand the karyotype diversification, we analyzed nine species of Trichomycterus using classical and molecular cytogenetic techniques. Results revealed a conserved diploid chromosome number of 2n=54 chromosomes in all analyzed species, although remarkable differences on the constitutive heterochromatin distribution were observed. In addition, while the 18S rDNA showed a conserved distribution pattern, the 5S rDNA sites showed a quite diverse location considering the analyzed species. Remarkably, both ribosomal genes were co-located in all species, except in T . iheringi , suggesting that co-localization is probably an ancestral condition in Trichomycterus . Finally, three analyzed species showed heterochromatic B chromosomes, reinforcing the intense genomic reorganization occurring in Trichomycterus . Our results showed that chromosomal variations are not restricted to differences in karyotype formula as previously proposed, but also to modifications on the microstructural level of resolution. |
id |
SBI-1_1062f898d750f4a28692d754ca75520c |
---|---|
oai_identifier_str |
oai:scielo:S1679-62252016000200211 |
network_acronym_str |
SBI-1 |
network_name_str |
Neotropical ichthyology (Online) |
repository_id_str |
|
spelling |
Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae)Karyotype diversityRepetitive DNArDNASupernumerary chromosomesTrichomycterus is a specious fish genus within Trichomycterinae and displays remarkable karyotype diversity. However, knowledge about their genomic structure and location of repetitive sequence is still limited. In order to better understand the karyotype diversification, we analyzed nine species of Trichomycterus using classical and molecular cytogenetic techniques. Results revealed a conserved diploid chromosome number of 2n=54 chromosomes in all analyzed species, although remarkable differences on the constitutive heterochromatin distribution were observed. In addition, while the 18S rDNA showed a conserved distribution pattern, the 5S rDNA sites showed a quite diverse location considering the analyzed species. Remarkably, both ribosomal genes were co-located in all species, except in T . iheringi , suggesting that co-localization is probably an ancestral condition in Trichomycterus . Finally, three analyzed species showed heterochromatic B chromosomes, reinforcing the intense genomic reorganization occurring in Trichomycterus . Our results showed that chromosomal variations are not restricted to differences in karyotype formula as previously proposed, but also to modifications on the microstructural level of resolution.Sociedade Brasileira de Ictiologia2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211Neotropical Ichthyology v.14 n.2 2016reponame:Neotropical ichthyology (Online)instname:Sociedade Brasileira de Ictiologia (SBI)instacron:SBI10.1590/1982-0224-20150084info:eu-repo/semantics/openAccessOliveira,Maria Lígia M. deUtsunomia,RicardoPansonato-Alves,José CarlosScacchetti,Priscilla C.Primo,Cleberson C.Vicari,Marcelo R.Artoni,Roberto F.Centofante,LianoMoreira-Filho,OrlandoOliveira,ClaudioForesti,Faustoeng2016-07-04T00:00:00Zoai:scielo:S1679-62252016000200211Revistahttp://www.ufrgs.br/ni/https://old.scielo.br/oai/scielo-oai.php||neoichth@nupelia.uem.br1982-02241679-6225opendoar:2016-07-04T00:00Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI)false |
dc.title.none.fl_str_mv |
Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae) |
title |
Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae) |
spellingShingle |
Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae) Oliveira,Maria Lígia M. de Karyotype diversity Repetitive DNA rDNA Supernumerary chromosomes |
title_short |
Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae) |
title_full |
Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae) |
title_fullStr |
Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae) |
title_full_unstemmed |
Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae) |
title_sort |
Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae) |
author |
Oliveira,Maria Lígia M. de |
author_facet |
Oliveira,Maria Lígia M. de Utsunomia,Ricardo Pansonato-Alves,José Carlos Scacchetti,Priscilla C. Primo,Cleberson C. Vicari,Marcelo R. Artoni,Roberto F. Centofante,Liano Moreira-Filho,Orlando Oliveira,Claudio Foresti,Fausto |
author_role |
author |
author2 |
Utsunomia,Ricardo Pansonato-Alves,José Carlos Scacchetti,Priscilla C. Primo,Cleberson C. Vicari,Marcelo R. Artoni,Roberto F. Centofante,Liano Moreira-Filho,Orlando Oliveira,Claudio Foresti,Fausto |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Maria Lígia M. de Utsunomia,Ricardo Pansonato-Alves,José Carlos Scacchetti,Priscilla C. Primo,Cleberson C. Vicari,Marcelo R. Artoni,Roberto F. Centofante,Liano Moreira-Filho,Orlando Oliveira,Claudio Foresti,Fausto |
dc.subject.por.fl_str_mv |
Karyotype diversity Repetitive DNA rDNA Supernumerary chromosomes |
topic |
Karyotype diversity Repetitive DNA rDNA Supernumerary chromosomes |
description |
Trichomycterus is a specious fish genus within Trichomycterinae and displays remarkable karyotype diversity. However, knowledge about their genomic structure and location of repetitive sequence is still limited. In order to better understand the karyotype diversification, we analyzed nine species of Trichomycterus using classical and molecular cytogenetic techniques. Results revealed a conserved diploid chromosome number of 2n=54 chromosomes in all analyzed species, although remarkable differences on the constitutive heterochromatin distribution were observed. In addition, while the 18S rDNA showed a conserved distribution pattern, the 5S rDNA sites showed a quite diverse location considering the analyzed species. Remarkably, both ribosomal genes were co-located in all species, except in T . iheringi , suggesting that co-localization is probably an ancestral condition in Trichomycterus . Finally, three analyzed species showed heterochromatic B chromosomes, reinforcing the intense genomic reorganization occurring in Trichomycterus . Our results showed that chromosomal variations are not restricted to differences in karyotype formula as previously proposed, but also to modifications on the microstructural level of resolution. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1982-0224-20150084 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
dc.source.none.fl_str_mv |
Neotropical Ichthyology v.14 n.2 2016 reponame:Neotropical ichthyology (Online) instname:Sociedade Brasileira de Ictiologia (SBI) instacron:SBI |
instname_str |
Sociedade Brasileira de Ictiologia (SBI) |
instacron_str |
SBI |
institution |
SBI |
reponame_str |
Neotropical ichthyology (Online) |
collection |
Neotropical ichthyology (Online) |
repository.name.fl_str_mv |
Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI) |
repository.mail.fl_str_mv |
||neoichth@nupelia.uem.br |
_version_ |
1752122182199148544 |