Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4685-GMB-2020-0027 http://hdl.handle.net/11449/208148 |
Resumo: | The cytogenetic characteristics of five fish species of the Moenkhausia are described, based on the analysis of specimens collected in different headwater. All the species analyzed presented 2n=50 chromosomes. The C-banding revealed a similar distribution pattern of heterochromatic blocks in all the species, except Moenkhausia nigromarginata. The 5S rDNA sites were distributed on multiple chromosome pairs in all five species. Single and multiple histone H1 sites were observed in all the species, and histone H1 was shown to be co-located with the 18S rRNA gene in a single chromosome pair. The U2 snDNA gene was distributed at multiple sites in all the Moenkhausia species. The presence of B microchromosomes was confirmed in Moenkhausia forestii, while individuals of the three study populations of Moenkhausia oligolepis presented three morphologically distinct types of B chromosome. The chromosomal mapping of the 18S rDNA sites using the FISH technique revealed signals in the B chromosomes ofM. forestii, while clusters of the H1 histone and U2 snDNA genes were found in the B chromosomes of M. forestii and M. oligolepis. The classical and molecular cytogenetic markers used in this study revealed ample variation in the Moenkhausia karyotypes, reflecting the dynamic nature of the chromosomal evolution. |
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Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae)Chromosomal mappingFish karyotypesMultigenic familiesRepetitive DNASupernumerary chromosomesThe cytogenetic characteristics of five fish species of the Moenkhausia are described, based on the analysis of specimens collected in different headwater. All the species analyzed presented 2n=50 chromosomes. The C-banding revealed a similar distribution pattern of heterochromatic blocks in all the species, except Moenkhausia nigromarginata. The 5S rDNA sites were distributed on multiple chromosome pairs in all five species. Single and multiple histone H1 sites were observed in all the species, and histone H1 was shown to be co-located with the 18S rRNA gene in a single chromosome pair. The U2 snDNA gene was distributed at multiple sites in all the Moenkhausia species. The presence of B microchromosomes was confirmed in Moenkhausia forestii, while individuals of the three study populations of Moenkhausia oligolepis presented three morphologically distinct types of B chromosome. The chromosomal mapping of the 18S rDNA sites using the FISH technique revealed signals in the B chromosomes ofM. forestii, while clusters of the H1 histone and U2 snDNA genes were found in the B chromosomes of M. forestii and M. oligolepis. The classical and molecular cytogenetic markers used in this study revealed ample variation in the Moenkhausia karyotypes, reflecting the dynamic nature of the chromosomal evolution.Universidade Estadual Paulista - UNESP Instituto de Biociências Departamento de Biologia Estrutural e FuncionalUniversidade do Estado de Mato Grosso - UNEMAT Departamento de Ciências BiológicasCentro Universitário Toledo - UNITOLEDOUniversidade Federal do Acre - UFAC Centro de Ciências Biológicas e NaturaisUniversidade Estadual Paulista Instituto de Biociências Departamento de Biologia Estrutural e Funcional, Rubião Junior S/NUniversidade Estadual Paulista - UNESP Instituto de Biociências Departamento de Biologia Estrutural e FuncionalUniversidade Estadual Paulista Instituto de Biociências Departamento de Biologia Estrutural e Funcional, Rubião Junior S/NUniversidade Estadual Paulista (Unesp)Universidade do Estado de Mato Grosso - UNEMATCentro Universitário Toledo - UNITOLEDOCentro de Ciências Biológicas e NaturaisDo Nascimento, Cristiano Neves [UNESP]Troy, Waldo PinheiroPansonato Alves, José CarlosCarvalho, Margarida LimaOliveira, Claudio [UNESP]Foresti, Fausto [UNESP]2021-06-25T11:07:14Z2021-06-25T11:07:14Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-9application/pdfhttp://dx.doi.org/10.1590/1678-4685-GMB-2020-0027Genetics and Molecular Biology, v. 43, n. 4, p. 1-9, 2020.1678-46851415-4757http://hdl.handle.net/11449/20814810.1590/1678-4685-GMB-2020-0027S1415-475720200006002052-s2.0-85096129232S1415-47572020000600205.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biologyinfo:eu-repo/semantics/openAccess2023-10-19T06:03:37Zoai:repositorio.unesp.br:11449/208148Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:17:16.860181Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae) |
title |
Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae) |
spellingShingle |
Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae) Do Nascimento, Cristiano Neves [UNESP] Chromosomal mapping Fish karyotypes Multigenic families Repetitive DNA Supernumerary chromosomes |
title_short |
Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae) |
title_full |
Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae) |
title_fullStr |
Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae) |
title_full_unstemmed |
Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae) |
title_sort |
Molecular cytogenetic analyses reveal extensive chromosomal rearrangements and novel B chromosomes in Moenkhausia (Teleostei, characidae) |
author |
Do Nascimento, Cristiano Neves [UNESP] |
author_facet |
Do Nascimento, Cristiano Neves [UNESP] Troy, Waldo Pinheiro Pansonato Alves, José Carlos Carvalho, Margarida Lima Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Troy, Waldo Pinheiro Pansonato Alves, José Carlos Carvalho, Margarida Lima Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade do Estado de Mato Grosso - UNEMAT Centro Universitário Toledo - UNITOLEDO Centro de Ciências Biológicas e Naturais |
dc.contributor.author.fl_str_mv |
Do Nascimento, Cristiano Neves [UNESP] Troy, Waldo Pinheiro Pansonato Alves, José Carlos Carvalho, Margarida Lima Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
dc.subject.por.fl_str_mv |
Chromosomal mapping Fish karyotypes Multigenic families Repetitive DNA Supernumerary chromosomes |
topic |
Chromosomal mapping Fish karyotypes Multigenic families Repetitive DNA Supernumerary chromosomes |
description |
The cytogenetic characteristics of five fish species of the Moenkhausia are described, based on the analysis of specimens collected in different headwater. All the species analyzed presented 2n=50 chromosomes. The C-banding revealed a similar distribution pattern of heterochromatic blocks in all the species, except Moenkhausia nigromarginata. The 5S rDNA sites were distributed on multiple chromosome pairs in all five species. Single and multiple histone H1 sites were observed in all the species, and histone H1 was shown to be co-located with the 18S rRNA gene in a single chromosome pair. The U2 snDNA gene was distributed at multiple sites in all the Moenkhausia species. The presence of B microchromosomes was confirmed in Moenkhausia forestii, while individuals of the three study populations of Moenkhausia oligolepis presented three morphologically distinct types of B chromosome. The chromosomal mapping of the 18S rDNA sites using the FISH technique revealed signals in the B chromosomes ofM. forestii, while clusters of the H1 histone and U2 snDNA genes were found in the B chromosomes of M. forestii and M. oligolepis. The classical and molecular cytogenetic markers used in this study revealed ample variation in the Moenkhausia karyotypes, reflecting the dynamic nature of the chromosomal evolution. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-25T11:07:14Z 2021-06-25T11:07:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4685-GMB-2020-0027 Genetics and Molecular Biology, v. 43, n. 4, p. 1-9, 2020. 1678-4685 1415-4757 http://hdl.handle.net/11449/208148 10.1590/1678-4685-GMB-2020-0027 S1415-47572020000600205 2-s2.0-85096129232 S1415-47572020000600205.pdf |
url |
http://dx.doi.org/10.1590/1678-4685-GMB-2020-0027 http://hdl.handle.net/11449/208148 |
identifier_str_mv |
Genetics and Molecular Biology, v. 43, n. 4, p. 1-9, 2020. 1678-4685 1415-4757 10.1590/1678-4685-GMB-2020-0027 S1415-47572020000600205 2-s2.0-85096129232 S1415-47572020000600205.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-9 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128493846790144 |