Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Neotropical ichthyology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000400831 |
Resumo: | In the present study it was showed for the first time the ultrastructural morphology of O. bonariensis sperm using electron microscopy techniques. Different kinds of abnormalities were described in fresh and post thawed sperm caused by crogenic protocols. Pejerrey spermatozoon is uniflagellated and is differentiated into three parts: a small roundish head (~1.80µm in length and 1.67µm in width), a midpiece or transitional region (~1.11µm in length and 1.56µm in width), and a long tail or flagellum (~29.08µm). Samples of fresh and post thawed sperm showed evidence of morphological anomalies affecting various intracellular compartments. Spermatozoa with swollen, ruptured, or absent membranes in the head showing excess of cytoplasm, and with alteration of the spatial orientation of the mitochondria were observed. A swollen flagellum was observed containing cytoplasmic vesicles, distributed along the whole length or concentrated in a restricted part of the tail. It was also found a high level of abnormalities (60%) in frozen sperm when compared with normal sperm (18%) reflecting the damage provoked by cryopreservation procedures. |
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Neotropical ichthyology (Online) |
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Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes)CryopreservationMorphologyIn the present study it was showed for the first time the ultrastructural morphology of O. bonariensis sperm using electron microscopy techniques. Different kinds of abnormalities were described in fresh and post thawed sperm caused by crogenic protocols. Pejerrey spermatozoon is uniflagellated and is differentiated into three parts: a small roundish head (~1.80µm in length and 1.67µm in width), a midpiece or transitional region (~1.11µm in length and 1.56µm in width), and a long tail or flagellum (~29.08µm). Samples of fresh and post thawed sperm showed evidence of morphological anomalies affecting various intracellular compartments. Spermatozoa with swollen, ruptured, or absent membranes in the head showing excess of cytoplasm, and with alteration of the spatial orientation of the mitochondria were observed. A swollen flagellum was observed containing cytoplasmic vesicles, distributed along the whole length or concentrated in a restricted part of the tail. It was also found a high level of abnormalities (60%) in frozen sperm when compared with normal sperm (18%) reflecting the damage provoked by cryopreservation procedures.Sociedade Brasileira de Ictiologia2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000400831Neotropical Ichthyology v.11 n.4 2013reponame:Neotropical ichthyology (Online)instname:Sociedade Brasileira de Ictiologia (SBI)instacron:SBI10.1590/S1679-62252013000400011info:eu-repo/semantics/openAccessGárriz,ÁngelaMiranda,Leandro A.eng2015-03-17T00:00:00Zoai:scielo:S1679-62252013000400831Revistahttp://www.ufrgs.br/ni/https://old.scielo.br/oai/scielo-oai.php||neoichth@nupelia.uem.br1982-02241679-6225opendoar:2015-03-17T00:00Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI)false |
dc.title.none.fl_str_mv |
Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes) |
title |
Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes) |
spellingShingle |
Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes) Gárriz,Ángela Cryopreservation Morphology |
title_short |
Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes) |
title_full |
Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes) |
title_fullStr |
Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes) |
title_full_unstemmed |
Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes) |
title_sort |
Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes) |
author |
Gárriz,Ángela |
author_facet |
Gárriz,Ángela Miranda,Leandro A. |
author_role |
author |
author2 |
Miranda,Leandro A. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Gárriz,Ángela Miranda,Leandro A. |
dc.subject.por.fl_str_mv |
Cryopreservation Morphology |
topic |
Cryopreservation Morphology |
description |
In the present study it was showed for the first time the ultrastructural morphology of O. bonariensis sperm using electron microscopy techniques. Different kinds of abnormalities were described in fresh and post thawed sperm caused by crogenic protocols. Pejerrey spermatozoon is uniflagellated and is differentiated into three parts: a small roundish head (~1.80µm in length and 1.67µm in width), a midpiece or transitional region (~1.11µm in length and 1.56µm in width), and a long tail or flagellum (~29.08µm). Samples of fresh and post thawed sperm showed evidence of morphological anomalies affecting various intracellular compartments. Spermatozoa with swollen, ruptured, or absent membranes in the head showing excess of cytoplasm, and with alteration of the spatial orientation of the mitochondria were observed. A swollen flagellum was observed containing cytoplasmic vesicles, distributed along the whole length or concentrated in a restricted part of the tail. It was also found a high level of abnormalities (60%) in frozen sperm when compared with normal sperm (18%) reflecting the damage provoked by cryopreservation procedures. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000400831 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000400831 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1679-62252013000400011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
dc.source.none.fl_str_mv |
Neotropical Ichthyology v.11 n.4 2013 reponame:Neotropical ichthyology (Online) instname:Sociedade Brasileira de Ictiologia (SBI) instacron:SBI |
instname_str |
Sociedade Brasileira de Ictiologia (SBI) |
instacron_str |
SBI |
institution |
SBI |
reponame_str |
Neotropical ichthyology (Online) |
collection |
Neotropical ichthyology (Online) |
repository.name.fl_str_mv |
Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI) |
repository.mail.fl_str_mv |
||neoichth@nupelia.uem.br |
_version_ |
1752122180921982976 |