Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)

Detalhes bibliográficos
Autor(a) principal: Santos,Celina Alcoforado
Data de Publicação: 2013
Outros Autores: Lenz,Dominik, Brandão,Geisamanda Pedrini, Chippari-Gomes,Adriana Regina, Gomes,Levy Carvalho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Neotropical ichthyology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193
Resumo: Diesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure.
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spelling Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)Comet assayCurimbaDieselMicronucleusDiesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure.Sociedade Brasileira de Ictiologia2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193Neotropical Ichthyology v.11 n.1 2013reponame:Neotropical ichthyology (Online)instname:Sociedade Brasileira de Ictiologia (SBI)instacron:SBI10.1590/S1679-62252013000100022info:eu-repo/semantics/openAccessSantos,Celina AlcoforadoLenz,DominikBrandão,Geisamanda PedriniChippari-Gomes,Adriana ReginaGomes,Levy Carvalhoeng2015-10-27T00:00:00Zoai:scielo:S1679-62252013000100193Revistahttp://www.ufrgs.br/ni/https://old.scielo.br/oai/scielo-oai.php||neoichth@nupelia.uem.br1982-02241679-6225opendoar:2015-10-27T00:00Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI)false
dc.title.none.fl_str_mv Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
title Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
spellingShingle Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
Santos,Celina Alcoforado
Comet assay
Curimba
Diesel
Micronucleus
title_short Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
title_full Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
title_fullStr Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
title_full_unstemmed Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
title_sort Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
author Santos,Celina Alcoforado
author_facet Santos,Celina Alcoforado
Lenz,Dominik
Brandão,Geisamanda Pedrini
Chippari-Gomes,Adriana Regina
Gomes,Levy Carvalho
author_role author
author2 Lenz,Dominik
Brandão,Geisamanda Pedrini
Chippari-Gomes,Adriana Regina
Gomes,Levy Carvalho
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos,Celina Alcoforado
Lenz,Dominik
Brandão,Geisamanda Pedrini
Chippari-Gomes,Adriana Regina
Gomes,Levy Carvalho
dc.subject.por.fl_str_mv Comet assay
Curimba
Diesel
Micronucleus
topic Comet assay
Curimba
Diesel
Micronucleus
description Diesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1679-62252013000100022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
dc.source.none.fl_str_mv Neotropical Ichthyology v.11 n.1 2013
reponame:Neotropical ichthyology (Online)
instname:Sociedade Brasileira de Ictiologia (SBI)
instacron:SBI
instname_str Sociedade Brasileira de Ictiologia (SBI)
instacron_str SBI
institution SBI
reponame_str Neotropical ichthyology (Online)
collection Neotropical ichthyology (Online)
repository.name.fl_str_mv Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI)
repository.mail.fl_str_mv ||neoichth@nupelia.uem.br
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