Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Neotropical ichthyology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193 |
Resumo: | Diesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure. |
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Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae)Comet assayCurimbaDieselMicronucleusDiesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure.Sociedade Brasileira de Ictiologia2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193Neotropical Ichthyology v.11 n.1 2013reponame:Neotropical ichthyology (Online)instname:Sociedade Brasileira de Ictiologia (SBI)instacron:SBI10.1590/S1679-62252013000100022info:eu-repo/semantics/openAccessSantos,Celina AlcoforadoLenz,DominikBrandão,Geisamanda PedriniChippari-Gomes,Adriana ReginaGomes,Levy Carvalhoeng2015-10-27T00:00:00Zoai:scielo:S1679-62252013000100193Revistahttp://www.ufrgs.br/ni/https://old.scielo.br/oai/scielo-oai.php||neoichth@nupelia.uem.br1982-02241679-6225opendoar:2015-10-27T00:00Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI)false |
dc.title.none.fl_str_mv |
Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae) |
title |
Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae) |
spellingShingle |
Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae) Santos,Celina Alcoforado Comet assay Curimba Diesel Micronucleus |
title_short |
Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae) |
title_full |
Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae) |
title_fullStr |
Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae) |
title_full_unstemmed |
Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae) |
title_sort |
Acute toxicity of the water-soluble fraction of diesel in Prochilodus vimboidesKner (Characiformes: Prochilodontidae) |
author |
Santos,Celina Alcoforado |
author_facet |
Santos,Celina Alcoforado Lenz,Dominik Brandão,Geisamanda Pedrini Chippari-Gomes,Adriana Regina Gomes,Levy Carvalho |
author_role |
author |
author2 |
Lenz,Dominik Brandão,Geisamanda Pedrini Chippari-Gomes,Adriana Regina Gomes,Levy Carvalho |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Santos,Celina Alcoforado Lenz,Dominik Brandão,Geisamanda Pedrini Chippari-Gomes,Adriana Regina Gomes,Levy Carvalho |
dc.subject.por.fl_str_mv |
Comet assay Curimba Diesel Micronucleus |
topic |
Comet assay Curimba Diesel Micronucleus |
description |
Diesel oil can be a source of contamination in aquatic environments, mainly as a result of spills. The effects of the water-soluble fraction of diesel (WSF) on Prochilodus vimboideswere assessed. Fish were exposed to three different WSF dilutions for up to 96 h and were compared to a control group. Damages in the fragments of DNA were analyzed using the Comet assay. The presence of erytrocyts abnormalities was assessed by micronucleus test. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and the accumulation of copper in gills were also analyzed. Fish exposed for 96 h had higher rates of damage than those exposed for 24 h. There was no significant difference regarding the presence of micronuclei between exposed and control fish and between 24-h and 96-h exposures. For AST, no significant difference was observed between samples collected at the two exposure times. Fish exposed to a 1:100 dilution of WSF showed higher activity of the enzyme ALT than the control fish after a 24-h exposure period. There was no bioaccumulation of copper in the gills. We conclude that the genotoxic effects of WSF in the cells are more evident in P. vimboides during an acute exposure. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100193 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1679-62252013000100022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
dc.source.none.fl_str_mv |
Neotropical Ichthyology v.11 n.1 2013 reponame:Neotropical ichthyology (Online) instname:Sociedade Brasileira de Ictiologia (SBI) instacron:SBI |
instname_str |
Sociedade Brasileira de Ictiologia (SBI) |
instacron_str |
SBI |
institution |
SBI |
reponame_str |
Neotropical ichthyology (Online) |
collection |
Neotropical ichthyology (Online) |
repository.name.fl_str_mv |
Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI) |
repository.mail.fl_str_mv |
||neoichth@nupelia.uem.br |
_version_ |
1752122180791959552 |