Sewage sludge does not induce genotoxicity and carcinogenesis

Detalhes bibliográficos
Autor(a) principal: Silva,Paula Regina Pereira
Data de Publicação: 2012
Outros Autores: Barbisan,Luis Fernando, Dagli,Maria Lúcia Zaidan, Saldiva,Paulo Hilário Nascimento
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400017
Resumo: Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group.
id SBG-1_40da68dd3fb710316368a247048d3012
oai_identifier_str oai:scielo:S1415-47572012000400017
network_acronym_str SBG-1
network_name_str Genetics and Molecular Biology
repository_id_str
spelling Sewage sludge does not induce genotoxicity and carcinogenesiscomet assaymicronucleussewage genotoxicityThrough a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group.Sociedade Brasileira de Genética2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400017Genetics and Molecular Biology v.35 n.3 2012reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572012005000054info:eu-repo/semantics/openAccessSilva,Paula Regina PereiraBarbisan,Luis FernandoDagli,Maria Lúcia ZaidanSaldiva,Paulo Hilário Nascimentoeng2012-08-10T00:00:00Zoai:scielo:S1415-47572012000400017Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2012-08-10T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Sewage sludge does not induce genotoxicity and carcinogenesis
title Sewage sludge does not induce genotoxicity and carcinogenesis
spellingShingle Sewage sludge does not induce genotoxicity and carcinogenesis
Silva,Paula Regina Pereira
comet assay
micronucleus
sewage genotoxicity
title_short Sewage sludge does not induce genotoxicity and carcinogenesis
title_full Sewage sludge does not induce genotoxicity and carcinogenesis
title_fullStr Sewage sludge does not induce genotoxicity and carcinogenesis
title_full_unstemmed Sewage sludge does not induce genotoxicity and carcinogenesis
title_sort Sewage sludge does not induce genotoxicity and carcinogenesis
author Silva,Paula Regina Pereira
author_facet Silva,Paula Regina Pereira
Barbisan,Luis Fernando
Dagli,Maria Lúcia Zaidan
Saldiva,Paulo Hilário Nascimento
author_role author
author2 Barbisan,Luis Fernando
Dagli,Maria Lúcia Zaidan
Saldiva,Paulo Hilário Nascimento
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva,Paula Regina Pereira
Barbisan,Luis Fernando
Dagli,Maria Lúcia Zaidan
Saldiva,Paulo Hilário Nascimento
dc.subject.por.fl_str_mv comet assay
micronucleus
sewage genotoxicity
topic comet assay
micronucleus
sewage genotoxicity
description Through a series of experiments, the genotoxic/mutagenic and carcinogenic potential of sewage sludge was assessed. Male Wistar rats were randomly assigned to four groups: Group 1 - negative control; Group 2 - liver carcinogenesis initiated by diethylnitrosamine (DEN; 200 mg/kg i.p.); Group 3 and G4- liver carcinogenesis initiated by DEN and fed 10,000 ppm or 50,000 ppm of sewage sludge. The animals were submitted to a 70% partial hepatectomy at the 3rd week. Livers were processed for routine histological analysis and immunohistochemistry, in order to detect glutathione S-transferase positive altered hepatocyte foci (GST-P+ AHF). Peripheral blood samples for the comet assay were obtained from the periorbital plexus immediately prior to sacrificing. Polychromatic erythrocytes (PCEs) were analyzed in femoral bone-marrow smears, and the frequencies of those micronucleated (MNPCEs) registered. There was no sewage-sludge-induced increase in frequency of either DNA damage in peripheral blood leucocytes, or MNPCEs in the femoral bone marrow. Also, there was no increase in the levels of DNA damage, in the frequency of MNPCEs, and in the development of GST-P AHF when compared with the respective control group.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572012005000054
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.35 n.3 2012
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
_version_ 1752122385070292992