Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates

• Autor(a) principal: Menezes,Caroline Anunciação
• Data de Publicação: 2003
• Outros Autores: Gonçalves,Danielle Silva, Amianti,Jackeline, Fernandes,Irene, Taddei,Carla Romano, Koga,Paula Célia Mariko, Trabulsi,Luiz Rachid, Martinez,Marina Baquerizo, Piazza,Roxane Maria Fontes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004
• topic: detection; polyclonal antibody; monoclonal antibody; thermo-labile toxin; E. coli

A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.